- Bee Products Chemical Analysis
- Insect and Pesticide Research
- Essential Oils and Antimicrobial Activity
- Plant and animal studies
- Healthcare and Venom Research
- Phytochemicals and Antioxidant Activities
- Electrochemical Analysis and Applications
- Conducting polymers and applications
- Agricultural and Food Sciences
- Insect and Arachnid Ecology and Behavior
- Analytical Chemistry and Sensors
- Meat and Animal Product Quality
- Horticultural and Viticultural Research
- Fungal Biology and Applications
- Insect Pest Control Strategies
- Insect Utilization and Effects
- Plant Reproductive Biology
- Phytochemistry Medicinal Plant Applications
- Antioxidant Activity and Oxidative Stress
- Free Radicals and Antioxidants
- Identification and Quantification in Food
- Advanced Chemical Sensor Technologies
- Synthesis and biological activity
- Mediterranean and Iberian flora and fauna
- Advancements in Transdermal Drug Delivery
Polytechnic Institute of Bragança
2016-2025
Secretaria Municipal de Cultura
2017
Chartered Institute of Management Accountants
2015
Instituto Politécnico Nacional
2005-2013
Universidade do Porto
1997-2005
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2000-2003
University of Leicester
1997-2003
Laboratoire d'Electrochimie Moléculaire
2000-2003
CEA Grenoble
2000-2003
Rede de Química e Tecnologia
2003
ABSTRACT Introduction Propolis is a chemically complex resinous substance collected by honeybees ( Apis mellifera ) from tree buds, comprising plant exudates, secreted substances bee metabolism, pollen and waxes. Its chemical composition depends strongly on the sources available around beehive, which have direct impact in quality bioactivity of propolis. Being as Portugal country botanical diversity, phenolic characterisation propolis different regions priority. Objective Extensive...
Propolis is one of the most fascinating honey bee (Apis mellifera L.) products. It a plant derived product that bees produce from resins they collect different organs and with which mix beeswax. building material protective agent in hive. also plays an important role social immunity, widely used by humans as ingredient nutraceuticals, over-the-counter preparations cosmetics. Its chemical composition varies geographic location, climatic zone local flora. The understanding diversity propolis...
Developing biodegradable active films has been a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization. This study aimed develop based on chitosan (CS), produced from waste crayfish (Procambarus clarkii) shells enriched with bioactive extract (5-20%) of propolis (PS) characterize its properties, envisaging food packaging applications. The chromatographic profile PS confirmed richness, 41 phenolic compounds. With...
Bee bread (BB) is a fermented mixture of plant pollen, honey, and bee saliva that worker bees use as food for larvae, young to produce royal jelly. In the present study, five BB samples, collected from Apis mellifera iberiensis hives located in different apiaries near Bragança, northeast region Portugal, one commercial sample were characterized by high-performance liquid chromatography coupled diode array detector electrospray mass spectrometry (HPLC-DAD-ESI/MS) terms phenolic compounds,...
Bee pollen (BP) and bee bread (BB) have been often investigated as potential functional foods. Both products are generally characterized by their high nutritional content, with BB being referred more digestible than BP, however, there is a lack of scientific studies proving this claim. Here, we present comparative evaluation the macronutrient digestibility BP after applying simulated in vitro gastrointestinal digestive system, together its value chemical composition. The scores for protein...
Bee pollen is frequently characterized as a natural source of bioactive components, such phenolic compounds, which are responsible for its pharmaceutical potential and nutritional properties. In this study, we evaluated the compound contents mono- polyfloral bee samples using spectroscopic chromatographic methods established links with their antioxidant antitumor activity. The findings demonstrated that botanical origin has remarkable impact on (3-17 mg GAE/g) flavonoid (0.5-3.2 QE/g)...
Oxidative electrochemical polymerization of (N,N '-ethylenebis(salicylideneaminato)) nickel(II), [Ni(salen)], in acetonitrile/TEAP was reinvestigated. The polymers were characterized by situ FTIR and UV−visible spectroscopies order to explore film structure clarify the electronic states as a function electrochemically controlled applied redox potential; oxidized species involved oxidative switching polymer also assessed ex EPR experiments. Integration data from all techniques revealed that...
Bee pollen and bee bread have always been regarded as excellent natural resources for application in food pharmaceutical fields due to their rich nutrient content diversity of bioactive compounds with health-improving properties. Extensive studies on both products ingredients a healthy diet were reported, although the data concerning metabolization gastrointestinal tract is quite limited. Here, we report, at each digestive stage, profile products, bioaccessibility levels antioxidant activity...
5-fluorouracil (5-FU) and doxorubicin (DOX) are potent anti-tumour agents commonly used for colon breast cancer therapy, respectively. However, their clinical application is limited by side effects the development of drug resistance. Honeybee venom a complex mixture substances that has been reported to be effective against different cells. Its active compound melittin, positively charged amphipathic peptide interacts with phospholipids cell membrane, forming pores enable internalization...
The production and design of innovative in-body microcarrier platforms for the controlled delivery bioactive substances to predefined sites continue hold substantial promise biotechnology medicine by increasing their therapeutic benefits. In this scope, plant pollen-based biocapsules, sporopollenin structures, have emerged as an alternative synthetic ones due low-cost, highly uniform size distribution, resistance physical chemical conditions, renewable green sources. Sporopollenin-based...
The nickel(II) complex with H2saltMe, a N2O2 Schiff base ligand derived from salicylaldehyde, was oxidatively electropolymerized on Pt electrodes in CH3CN/0.1 mol dm-3 tetraethylammonium perchlorate (TEAP) to generate polymer films that exhibit reversible oxidative electrochemical behavior wide potential range (0.0−1.3 V), high conductivity, and stability/durability. of poly[Ni(saltMe)] can be made the three regimes charge transport by manipulation film thickness experimental time scale....
Propolis is a beehive product with very complex chemical composition, used since ancient times in several therapeutic treatments. As contribution to the improvement of drugs against tropical diseases caused by protozoa, we screened Portuguese propolis and its potential floral sources Populus x Canadensis Cistus ladanifer Plasmodium falciparum, Leishmania infantum, Trypanosoma brucei cruzi. The toxicity MRC-5 fibroblast cells was evaluated assess selectivity. vitro assays were performed...
Beebread is a honey bee product resulting from the storage and lactic fermentation of pollen within hive. The potential use beebread as food supplement nutraceutical greatly depends on its chemical richness, which varies upon flora diversity region season collection by bees. To evaluate this source nutraceuticals, we analyzed produced in northeast Portugal, along with commercial sample. analysis focused composition, nutritional parameters (moisture, ash, fats, proteins sugars), presence...