OBJECTIVE This study examines the differential expression of myosin heavy chain (MyHC) components in human laryngeal muscle groups. STUDY DESIGN A battery monospecific monoclonal antibodies Western blots was used to determine IIX, extraocular‐specific (EOM), and IIB MyHCs for thyroarytenoid (TA), vocalis (VOC), lateral cricoarytenoid (LCA), cricothyroid (CT), posterior (PCA) muscles obtained from fresh cadaver specimens. RESULTS Fast IIX MyHC only expressed TA, VOC, LCA muscles. IIA slow...

ABSTRACT We analyzed the interaction between polyamines and outer membrane of Escherichia coli cells using potentiometric measurements with Ca 2+ , tetraphenylphosphonium (TPP + ), K electrodes. The electrode was used to examine ability release from membrane. TPP permeabilize membrane, since uptake enhanced when permeability barrier disrupted. permeabilization in cytoplasmic by monitoring efflux cytosol. Although remarkably increasing number amino groups polyamines, no observed a simple...

To evaluate how the viscosity of laryngeal mucus influences vocal fold vibration, two fluids differing were applied separately to excised canine larynges and experimental phonation was induced. Vibration folds measured by use a laryngostroboscope an X-ray stroboscope. With high fluid, amplitude vibration free edge peak glottal area decreased while open quotient increased. Because this fluid affected wave motion mucosa, changes in may be involved causing such disorders as hoarseness, absence...

Abstract Objectives To test the hypothesis that myosin heavy chain (MHC) composition is a biological marker indicative of appropriate and functional reinnervation. Study Design Age‐matched adult rats were randomized for prospective study under three experimental conditions. Methods In rats, e‐perimental conditions surgically created, including transient recurrent laryngeal nerve (RLN) crush injury, RLN transection repair, cricoarytenoid joint fi‐ation with intact RLN. Animals survived 30,...

SUMMARY Cryptosporidium parvum, belonging to the phylum Apicomplexa, is a major cause of waterborne gastroenteritis throughout world. The sporozoites are thought invade host enterocytes using an active process termed gliding motility. However, biological and morphological changes within during this not fully understood. In present study, excysted C. parvum were analysed ultrastructurally in vitro their viability was evaluated fluorescent dyes. from oocysts changed morphologically...

To determine whether the myotrophic activity of human insulin-like growth factor (hIGF)-1 promotes restoration normal myosin heavy chain (MHC) composition after nerve injury, MHC was analyzed hIGF-1 gene transfer in denervated rat laryngeal muscle.Animal model to study effects on paralysis.In anesthetized rats, left recurrent and superior nerves are cut suture ligated. A midline thyrotomy is performed, thyroarytenoid muscle injected with a polyvinyl-based formulation containing specific...

Abstract Objectives/Hypothesis The authors have designed a rat laryngeal paralysis model to study gene transfer strategies using muscle‐specific expression system enhance local delivery of human insulin‐like growth factor‐1 (hIGF‐1). In preliminary studies, nonviral vector containing the α‐actin promoter and hIGF‐1 sequence produced both neurotrophic myotrophic effects 1 month after single injection plasmid formulation into paralyzed thyroarytenoid muscle in vivo. Based on these findings, it...

Glossopharyngeal nerve paralysis caused by varicella zoster virus reactivation is rare. We present a case of glossopharyngeal confirmed direct immunofluorescence staining for antigens. A 35-year-old man presented with right-sided, severe swallowing pain and dysgeusia. Physical examination showed loss ipsilateral gag reflex. White spots on the posterior wall right pyriform sinus were seen laryngofibroscopy, taste part tongue was gustometry using filter paper disc method. The antigen revealed...

SUMMARY Cryptosporidium parvum is an intracellular protozoan parasite belonging to the phylum Apicomplexa, and a major cause of waterborne gastroenteritis throughout world. Invasive zoites apicomplexan parasites, including C. , are thought have characteristic organelles on apical apex; however, compared with other cytoskeletal ultrastructure poorly understood. Thus, in present study, we ultrastructurally examined sporozoites using electron microscopy clarify framework invasive stages....

Abstract Objectives Recent technology manufactured a nasopharyngeal videoscope with pistol‐shaped grip (PG). This study aimed to assess the ergonomic feasibility of this novel device in daily ENT practice. Methods To impact shape on physicians, conventional (CG) and PG were utilized study. Surface electromyography (sEMG) was recorded muscle activity upper limb during endoscopy training model. Bilateral sEMG recordings performed including thenar muscle, pronator teres brachioradialis biceps...

We report a 64-year-old woman who developed endogenous endophthalmitis in the course of severe periodontitis. Histopathological examination ofthe vitreous debris obtained during vitrectomy disclosed fungi and its culture identified Aspergillus flavus. Periodontitis should be considered as one infectious foci for fungal well bacterial endophthalmitis.

We demonstrate a 40-Gbit/s receiver using semiconductor optical amplifier and discuss the advantage over avalanche photodiode receiver. Measured minimum sensitivity was -21.3 dBm, which is highest ever reported without fiber amplifiers.

Unanticipated difficult airway is a challenging problem for anesthesiologists. Oropharyngeal stenosis (OPS) rare complication of upper surgery which may cause airway. We present patient whose postsurgical OPS was revealed during the induction general anesthesia, and necessitated reschedule tracheotomy. also discuss etiology risk factors OPS.

The Raman spectra (300–2000 cm−1) of five nerve gases, one gas precursor, blister agents, three lachrymators, vomit agent and choking seven chemical warfare agents (CWAs) simulants as liquid or solid state were measured using portable spectrometer (Delta Nu Co., RAPID ID, excitation wavelength: 785 nm). Generally, characteristic composed sharp peaks observed, but for nitrogen mustard 3 adamsite provided broad spectra. library search presented correct names against only CWAs. Sarin (GB) (HD)...

Abstract In the frog optic tectum, spatiotemporal pattern of neuronal activity evoked by electrical stimulation tract was examined means a current source density (CSD) analysis. The CSD depth profile highly reproducible in different experiments. all seven profiles, three sinks A, B, and D were observed retinorecipient layers. Four out profiles show additional two C E below Very small short lasting related to afferent fiber activities precede A B about 1 ms, which could be accounted for...