We had previously investigated the expression and functional role of C-X-C Motif Chemokine Ligand 12 (CXCL12) during hair cycle progression. CXCL12 was highly expressed in stromal cells such as dermal fibroblasts (DFs) inhibition increased growth. Therefore, we further whether a neutralizing antibody (αCXCL12) is effective for androgenic alopecia (AGA) areata (AA) studied underlying molecular mechanism treating these diseases. In AGA model, DFs. Subcutaneous (s.c.) injection αCXCL12...

The regulatory mechanisms and functional roles of agonist-induced internalization G protein-coupled receptors (GPCRs) were analyzed using mutant dopamine D(2) (D(2)Rs) in which all possible GPCR kinase (GRK) phosphorylation sites mutated or the affinity for beta-arrestins was altered. Agonist-induced D(2)Rs involved a phosphorylation-dependent component, mediated by serine/threonine (S/T) residues second loop T225 third loop, phosphorylation-independent component. GRK2-mediated enhancement...

In addition to typical GPCR kinase (GRK)-/β-arrestin-dependent internalization, dopamine D3 receptor employed an additional GRK-independent sequestration pathway. this study, we investigated the molecular mechanism of novel pathway.Radioligand binding, flow cytometry and cell surface biotinylation assay were used characterize trafficking properties D2 receptors. Serine/threonine N-linked glycosylation mutants utilized locate regions involved in pharmacological desensitization. Various point...

Adipose tissue is a specialized organ that synthesizes and stores fat. During adipogenesis, Rho Rho-associated kinase (ROCK) 2 are inactivated, which enhances the expression of pro-adipogenic genes induces loss actin stress fibers. Furthermore, pan ROCK inhibitors enhance adipogenesis in 3T3-L1 cells. Here, we show KD025 (formerly known as SLx-2119), ROCK2-specific inhibitor, suppresses cells partially through ROCK2-independent mechanism. downregulated key adipogenic transcription factors...

BackgroundDermal papilla cells (DPCs) play a key role in hair regeneration and morphogenesis. Therefore, tremendous efforts have been made to promote DPC inductivity.

Abstract Background Predicting short-term efficacy and intracranial progression-free survival (iPFS) in epidermal growth factor receptor gene mutated (EGFR-mutated) lung adenocarcinoma patients with brain metastases who receive third-generation tyrosine kinase inhibitor (EGFR-TKI) therapy was of great significance for individualized treatment. We aimed to construct validate nomograms based on clinical characteristics magnetic resonance imaging (MRI) radiomics predicting progression free...

Functional roles of the N-terminal region rhodopsin-like GPCR family remain unclear. Using dopamine D(2) and D(3) receptors as a model system, we probed in signalling, intracellular trafficking receptor proteins, explored critical factors that determine functionality region.The was gradually shortened or switched with non-specific sequence (FLAG), potential glycosylation sites were mutated. Effects these manipulations on surface expression, internalization, post-endocytic behaviours...

We have previously demonstrated that receptor-interacting serine threonine kinase 1 (RIPK1) is expressed in hair follicles and regulates the cycle. In a mouse model, RIPK1 inhibitors also accelerated telogen-to-anagen transition elongated anagen period. Here, we first investigated involvement of alopecia areata (AA). The mRNA protein expression was increased skin an AA model. Single-cell RNA sequencing immunohistochemistry showed highly dendritic cells (DCs) CD8+ T cells. (i.e.,...

Together with G protein-coupled receptor (GPCR) kinases (GRKs) and β-arrestins, RGS proteins are the major family of molecules that control signaling GPCRs. The expression pattern one these members, RGS9-2, coincides dopamine D3 (D3R) in brain, vivo studies have shown RGS9-2 regulates D2-like receptors. In this study, β-arrestin2 was found to be required for scaffolding intricate interactions among dishevelled-EGL10-pleckstrin (DEP) domain Gβ5, R7-binding protein (R7BP), D3R. DEP under...

GPCRs undergo both homologous and heterologous regulatory processes in which receptor phosphorylation plays a critical role. The protein kinases responsible for each pathway are well established; however, other molecular details that characterize remain unclear. In this study, the mechanisms determine differences functional roles intracellular trafficking between PKC-mediated internalization pathways dopamine D₂ were investigated.

Abstract This study is aimed to explore the regulatory effect of lnc RNA HOTAIR /miR‐148a/ DLGAP 1 axis on head and neck tumor ( HNT ) cell growth, mobility, invasiveness. HOTAIRM 1, miR‐148a, level in tissues adjacent normal were measured by qRT ‐ PCR . Cell Counting Kit‐8 CCK ‐8) Transwell (migration invasion) assay used survey influence Fadu cells. Nude mouse xenograft was utilized validate vivo. Dual‐luciferase reporter confirms relationship between The expression downregulated human...

The tumorigenesis and metastasis of tumors are associated with human collagen triple helix repeats containing 1 (CTHRC1). To study the effects possible impacting mechanisms CTHRC1 on cervical carcinoma development, samples paraffin‑embedded HeLa cells were examined. Immunofluorescence, cell wound scratch assay, western blot analysis Transwell invasion assay used to evaluate in response silencing gene carcinoma. expression levels gap‑associated proteins Wnt/PCP pathway also evaluated by...

Nursing handovers are a critical component of patient safety. Researchers have performed many primary studies in this field, mainly reporting findings from changes nursing handover patterns. However, few quantitative explored the factors that influence quality. Therefore, study aimed to investigate quality and explore associations between quality, job satisfaction, group cohesion among psychiatric nurses.This cross-sectional included 186 registered nurses Chinese hospital, who responded...

We previously demonstrated that C-X-C Motif Chemokine Ligand 12 (CXCL12) is primarily secreted by dermal fibroblasts in response to androgens and induces hair miniaturization the mouse androgenic alopecia (AGA) model. However, direct effects of androgen-induced CXCL12 on papilla cells (DPCs) sheath cup (DSCs) have not been demonstrated. First, we compared single-cell RNA sequencing data between human skin, results show highly co-expressed with androgen receptor (AR) DPCs DSCs only hair....

GPCR kinase 2 (GRK2)/β-arrestins and protein A (PKA)/protein C (PKC) mediate homologous heterologous regulations of GPCRs, respectively. Conventional enzymes (PKCs), as exemplified by PKCβII, selectively inhibit internalization dopamine D2 receptor β2 adrenoceptor in a β-arrestin- but not GRK2-dependent manner. PKCβII interacts with β-arrestin2 upon autophosphorylation at T250, inhibits the decreasing ubiquitination β-arrestin2. interferes interaction between MDM2 cytosol, resulting...