A5054879877- Gut microbiota and health
- Infant Nutrition and Health
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Respiratory viral infections research
- Pediatric health and respiratory diseases
- Plant Virus Research Studies
- Infant Health and Development
- Pediatric Hepatobiliary Diseases and Treatments
- Pelvic floor disorders treatments
- Urinary Tract Infections Management
- Diet and metabolism studies
- Birth, Development, and Health
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Plant and Fungal Interactions Research
- Pregnancy and preeclampsia studies
- Breastfeeding Practices and Influences
- Phagocytosis and Immune Regulation
- Clostridium difficile and Clostridium perfringens research
- Neonatal Respiratory Health Research
- Digestive system and related health
- Reproductive System and Pregnancy
- Immune Cell Function and Interaction
- Genetics and Neurodevelopmental Disorders
University of Groningen
2020-2024
University Medical Center Groningen
2021-2024
Linköping University
2018-2021
Philipps University of Marburg
2016
Abstract Early development of the gut ecosystem is crucial for lifelong health. While infant bacterial communities have been studied extensively, virome remains under-explored. To study over time and factors that shape it, we longitudinally assess composition viruses their hosts in 30 women during after pregnancy 32 infants first year life. Using shotgun metagenomic sequencing applied to dsDNA extracted from Virus-Like Particles (VLPs) bacteria, generate 205 VLP metaviromes 322 total...
Extremely low birth weight (ELBW) infants often develop an altered gut microbiota composition, which is related to clinical complications, such as necrotizing enterocolitis and sepsis. Probiotic supplementation may reduce these modulation of the microbiome a potential mechanism underlying probiotic effectiveness. In randomized, double-blind, placebo-controlled trial, we assessed effect Lactobacillus reuteri supplementation, from post-menstrual week (PMW)36, on infant microbiota. We performed...
The human gut microbiome consists of bacteria, archaea, eukaryotes, and viruses. viruses are relatively underexplored. Here, we longitudinally analyzed the virome composition in 11 healthy adults: its stability, variation, effect a gluten-free diet. Using viral enrichment de novo assembly-based approach, demonstrate quantitative dynamics virome, including dsDNA, ssDNA, dsRNA, ssRNA We observe highly divergent individual communities, carrying on an average 2,143 genomes, 13.1% which were...
During pregnancy, the semi-allogeneic nature of foetus requires maternal immune adaption and acquisition tolerance at foetal-maternal interface. Macrophages with regulatory properties T (Treg) cells are central in promoting foetal enriched decidua (the uterine endometrium during pregnancy). Although tissue-resident decidual stromal (DSC) have been implicated functions, it is not known if they able to induce phenotype macrophages T-cells. In this study we report that maternally derived DSC...
Abstract Human milk microbiome studies are currently hindered by low bacterial/human cell ratios and often rely on 16S rRNA gene sequencing, which limits downstream analyses. Here, we aimed to find a method study bacteria assess bacterial sharing between maternal infant microbiota. We tested four DNA isolation methods, two enrichment methods three sequencing mock communities, samples negative controls. Of the kits, DNeasy PowerSoil Pro (PS) MagMAX Total Nucleic Acid Isolation (MX) kits...
Large sex hormonal fluctuations are thought to influence vaginal microbiota, but little is known about the impact of small, physiological variations. Here we tracked changes in microbiota during four key menstrual cycle phases 61 healthy Italian women from Women4Health cohort. The was primarily composed Lactobacillus, with L. iners being most abundant. Noteworthy, high abundance contrasts previous studies European populations, challenging its proposed pathogenic role, and suggesting distinct...
Lactobacillus reuteri DSM 17938 supplementation reduces morbidities in very low birth weight infants (<1500 g), while the effect on extremely (ELBW, <1000 g) is still questioned. In a randomised placebo-controlled trial (ClinicalTrials.gov ID NCT01603368), head growth, but not feeding tolerance or morbidities, improved L. reuteri-supplemented preterm ELBW infants. Here, we investigate colonisation with probiotic strain who received placebo from to postmenstrual week (PMW) 36....
<title>Abstract</title> The gut microbiomes during pregnancy and infancy play a critical role in maternal infant health, yet our understanding of what determines their temporal dynamics, interactions, health impacts remains poor. In this study, we profiled 4,577 longitudinal fecal samples from 714 mother-infant pairs the Dutch birth cohort LLNEXT, 12 weeks to one year postpartum. Hospital records questionnaires were longitudinally evaluated assess 474 clinical, dietary, exposure variables,...
16S rRNA gene sequencing enables microbial community profiling, but recovering fecal DNA from extremely premature infants is challenging. Here, we describe an optimized protocol for isolation, library preparation sequencing, taxonomy assignation, and statistical analyses. The complemented with a quantitative PCR probiotic L. reuteri identification. This describes how to characterize preterm infant gut microbiota relate it supplementation clinical outcomes. It customizable other trials. For...
The human gut microbiome is a complex ecosystem of bacteria, archaea, eukaryotes, and viruses. composition dynamics the viruses, commonly termed virome, are relatively underexplored. We performed longitudinal analysis viral communities in 11 healthy adults order to study individual variations, virome stability, effect gluten-free diet (GFD). Using enrichment de novo assembly-based approach, we demonstrate quantitative including dsDNA, ssDNA, dsRNA, ssRNA observe that individual’s highly...
Abstract Early development of the gut ecosystem is crucial for lifelong health. While infant bacterial communities have been studied extensively, virome remains under-explored. We longitudinally assessed composition viruses and their hosts in 322 total metagenomes 205 metaviromes from 30 mothers during after pregnancy 32 infants first year life. maternal remained stable late birth, was dynamic life contained a higher abundance active temperate phages compared to viromes. The also influenced...
Abstract Human milk microbiome studies are currently hindered by low bacterial/human cell ratios and often rely on 16S rRNA gene sequencing, which limits downstream analyses. Here, we aimed to find a method study bacteria assess bacterial sharing between maternal infant microbiota. We tested four DNA isolation methods, two enrichment methods three sequencing mock communities, samples negative controls. Of the kits, DNeasy PowerSoil Pro (PS) MagMAX Total Nucleic Acid Isolation (MX) kits...
Abstract Seeding and development of the gut ecosystem are crucial for health, both in childhood later life. While composition infant bacterial communities has been described, origin virome remains under-studied. Here, we explore mother-to-infant transmission bacteria viruses 30 mother–infant pairs a longitudinal collection faecal samples taken during pregnancy first 3 months after birth. We demonstrate that strains resemble maternal more than those unrelated mothers. quantify viromes using...