A5100346188- Plant Molecular Biology Research
- Research in Cotton Cultivation
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Microbial Community Ecology and Physiology
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Genetics, Aging, and Longevity in Model Organisms
- Enzyme Catalysis and Immobilization
- Bacteriophages and microbial interactions
- Animal Virus Infections Studies
- Protist diversity and phylogeny
- Spaceflight effects on biology
- Plant nutrient uptake and metabolism
- Phytase and its Applications
- Genetic Mapping and Diversity in Plants and Animals
- Nuclear Structure and Function
- Wheat and Barley Genetics and Pathology
- Algal biology and biofuel production
- Plant Stress Responses and Tolerance
- Heat shock proteins research
- Viral gastroenteritis research and epidemiology
- Genetics and Plant Breeding
Chinese Academy of Agricultural Sciences
2016-2025
Biotechnology Research Institute
2016-2025
Beijing VDJBio (China)
2025
Southwest University of Science and Technology
2020-2024
Lanzhou University
2023
Lanzhou Veterinary Research Institute
2023
Chongqing University of Posts and Telecommunications
2016
Institute of Crop Sciences
2010-2011
Hebei Agricultural University
2010
Cotton is white gold across the globe and composed of fiber cells derived from outer integument cotton ovules. Fiber elongation uses sucrose as a direct carbon source. The molecular mechanism transcriptionally controlling transport ovules into elongating fibers remains elusive. In this study involvement GhMYB212 in regulation transportion expanding was investigated. RNAi plants (GhMYB212i) accumulated less glucose developing fibers, had shorter lower lint index. RNA-seq protein-DNA binding...
The desert is an excellent model for studying evolution under extreme environments. We present here the complete genome and ultraviolet (UV) radiation-induced transcriptome of Deinococcus gobiensis I-0, which was isolated from cold Gobi shows higher tolerance to gamma radiation UV light than all other known microorganisms. Nearly half genes in encode proteins unknown function, suggesting that resistance phenotype may be attributed pathways. D. also contains a surprisingly large number...
Annexins are assumed to be involved in regulating cotton fiber elongation, but direct evidence remains presented. Here we cloned six Annexin genes (AnxGb) abundantly expressed from sea-island (G. barbadense). qRT-PCR results indicated that all G. barbadense annexin were elongating fibers, while only the expression of AnxGb6 was fiber-specific. Yeast two hybridization and BiFC analysis revealed homodimer interacted with a specific actin GbAct1. Ectopic-expressed Arabidopsis enhanced its root...
Abscisic acid (ABA) signaling plays important roles in plant growth, development and adaptation to various stresses. RCAR1/PYL9 has been known as a cytoplasm nuclear ABA receptor Arabidopsis. To obtain further insight into the regulatory mechanism of RCAR1/PYL9, yeast two-hybrid approach was performed screen for RCAR1/PYL9-interacting proteins an R2R3-type MYB transcription factor, AtMYB44, identified. The interaction between AtMYB44 confirmed by glutathione S-transferase (GST) pull-down...
Abstract The cotton fibers are seed trichomes that elongate from the ovule epidermis. Polar lipids required for quick enlargement of cell membrane and fiber growth, however, how transported ovules into developing remains less known. Here, we reported functional characterization GhLTPG1, a GPI-anchored lipid transport protein, during elongation. GhLTPG1 was abundantly expressed in elongating outer integument protein located on membrane. Biochemical analysis showed specifically bound to...
Ethylene plays an essential role in the development of cotton fibres. biosynthesis plants is elaborately regulated by activities key enzymes, 1-aminocyclopropane-1-carboxylate oxidase (ACO) and synthase (ACS); however, potential mechanism post-translational modification ACO ACS to control ethylene synthesis fibres remains unclear. Here, we identify E3 ubiquitin ligase, GhXB38D, that regulates during fibre elongation cotton. GhXB38D gene highly expressed rapid stage. Suppressing expression...
Deinococcus radiodurans is a remarkably unique microorganism, exhibiting extraordinary tolerance to extreme conditions such as ionizing radiation, ultraviolet light, and desiccation. However, the response mechanisms of D. under low-temperature stress remain largely unexplored have yet be fully elucidated. The DohD protein hydrophilic member late embryogenesis abundant 3 (LEA3) family radiodurans, playing pivotal role in abiotic adaptation. Bioinformatics analysis revealed that contains...
Members of the Microvirga genus are metabolically versatile and widely distributed in Nature. However, knowledge bacteria that belong to this is currently limited biochemical characteristics. Herein, a novel thermo-tolerant bacterium named thermotolerans HR1 was isolated identified. Based on 16S rRNA gene sequence analysis, strain belonged highly similar sp. 17 mud 1-3. The could grow at temperatures ranging from 15 50 °C with growth optimum 40 °C. It exhibited tolerance pH range 6.0-8.0...
Abstract Water stress and hypersensitive response (WHy) domain is typically found as a component of atypical late embryogenesis abundant (LEA) proteins closely associated with resistance to multiple stresses in numerous organisms. Several putative LEA have been identified Deinococcus bacteria; however their precise function remains unclear. This work reports the characterization -specific gene encoding novel WHy domain-containing hydrophobic LEA5C protein (named DrwH) D . radiodurans R1. The...
Summary Late embryogenesis abundant ( LEA ) proteins play a protective role during desiccation and oxidation stresses. 3 are major group characterized by hydrophilic domain HD with highly conserved repeating 11‐amino acid motif. We compared four different orthologs from distant organisms: (i) Dr the extremophilic bacterium Deinococcus radiodurans ; (ii) Ce nematode Caenorhabditis elegans (iii) Yl yeast Yarrowia lipolytica (iv) Bn plant Brassica napus . Circular dichroism spectroscopy showed...
Deinococcus radiodurans adapts to challenging environments by modulating gene expression in response oxidative stress. Recently, bacterial small noncoding RNAs (sRNAs) have been presumed participate the transcriptional or translational regulation of stress-responsive genes. We found 24 sRNAs that may be involved stress D. deep RNA sequencing. Moreover, a typical stress-inducible sRNA, IGR_3053, named OsiA, was predicted bind mRNA katA, katE, and sodC bioinformatics method. An osiA knockout...
Waste oil pollution and the treatment of oily waste present a challenge, exploitation microbial resources is safe efficient method to resolve these problems. Lipase-producing microorganisms can directly degrade promote degradation and, therefore, have very significant research application value. The isolation oil-degrading strains great practical significance in into remediation oil-contaminated environments for enrichment lipase resource library. In this study, Acinetobacter junii WCO-9, an...
Acinetobacter calcoaceticus PHEA-2 utilizes phenol as its sole carbon and energy source has a multi-component hydroxylase-encoding gene operon (mphKLMNOP) for degradation. Two additional genes, mphR mphX, were found upstream downstream of mphKLMNOP, respectively. The encodes XylR/DmpR-type regulator-like protein is transcribed in the opposite direction to mphKLMNOP. mphX same mphKLMNOP with 293 amino acid residues showing weak identity some unknown proteins encoded meta-cleavage pathway...
Reactive oxygen species (ROS) can cause destructive damage to biological macromolecules and protein dysfunction in bacteria. Methionine sulfoxide reductase (Msr) with redox-active Cys and/or seleno-cysteine (Sec) residues restore physiological functions of the proteome, which is essential for oxidative stress tolerance extremophile Deinococcus radiodurans. However, underlying mechanism regulating MsrA enzyme activity D. radiodurans under has remained elusive. Here, we identified function...
Keratinase can specifically attack disulfide bridges in keratin to convert them from complex simplified forms. thermal stability has drawn attention various biotechnological industries. In this study, a keratinase DgeKer was identified slightly thermophilic species, D. geothermalis. The silico analysis showed that is composed of signal peptide, N-terminal propeptide, mature domain, and C-terminal extension. its extension-truncated enzyme (DgeKer-C) were cloned expressed E. coli. purified...
Keratin is a tough fibrous structural protein that difficult to digest with pepsin and trypsin because of the presence large number disulfide bonds. widely found in agricultural waste. In recent years, especially, development poultry industry has resulted accumulation feather keratin resources, which seriously pollute environment. Keratinase can specifically attack bridges keratin, converting them from complex simplified forms. The keratinase thermal stability drawn attention various...
To grow in various harsh environments, extremophiles have developed extraordinary strategies such as biofilm formation, which is an extremely complex and progressive process. However, the genetic elements exact mechanisms underlying extreme formation remain enigmatic. Here, we characterized biofilm-forming ability of Deinococcus radiodurans vitro under environmental conditions found that high concentrations NaCl or sorbitol could induce formation. Meantime, survival cells was superior to...
ABSTRACT Cotton fiber length is basically determined by well‐coordinated gene expression and phosphatidylinositol phosphates (PIPs) accumulation during elongation but the regulatory mechanism governing PIPs transport remains unknown. Here, we report a MYB transcription factor GhMYB30D04 in Gossypium hirsutum that promotes through modulating of PIP transporter GhLTPG1 . Knockout cotton (KO) results reduction transcripts with lower PIPs, leading to shorter fibers yield. Conversely,...
Small non-coding RNAs (ncRNAs) are a class of regulatory molecules, which remain understudied in bacteria. In the extremophilic bacterium Deinococcus radiodurans, although hundreds ncRNAs have been identified, few characterized detail. this study, we report identification and characterization novel heat-inducible ncRNA named DnrH. Heat tolerance analysis showed that deleting DnrH significantly inhibited viability response to high temperature conditions. Comparative phenotypic qRT-PCR...