A5070160093- Bone health and osteoporosis research
- Bone Tissue Engineering Materials
- Orthopaedic implants and arthroplasty
- Bone health and treatments
- Dental Implant Techniques and Outcomes
- Spectroscopy Techniques in Biomedical and Chemical Research
- Bone Metabolism and Diseases
- Advanced Polymer Synthesis and Characterization
- Synthesis and properties of polymers
- Advanced NMR Techniques and Applications
- Connective tissue disorders research
- NMR spectroscopy and applications
- Titanium Alloys Microstructure and Properties
- Bone fractures and treatments
- biodegradable polymer synthesis and properties
- Evolution and Science Education
- 3D Printing in Biomedical Research
- Calcium Carbonate Crystallization and Inhibition
- Bone and Dental Protein Studies
- Hydrogen embrittlement and corrosion behaviors in metals
- Advanced MRI Techniques and Applications
- Fatigue and fracture mechanics
- Photopolymerization techniques and applications
- Inorganic and Organometallic Chemistry
- Hip disorders and treatments
University of Michigan
2015-2025
Biolog (United States)
1992-2024
Materials Sciences (United States)
2024
Material Sciences (United States)
2010-2022
Asklepios Klinik Weißenfels
2020
Michigan United
2001-2015
National High Magnetic Field Laboratory
2010
Central Michigan University
2010
Drew University
1989-2005
Elisha Hospital
2000-2001
Strategies to engineer bone have focused on the use of natural or synthetic degradable materials as scaffolds for cell transplantation substrates guide regeneration. The basic requirements scaffold material are biocompatibility, degradability, mechanical integrity, and osteoconductivity. A major design problem is satisfying each these with a single material. This study addresses this by describing an approach combine biocompatibility degradability polymer osteoconductivity reinforcement...
Abstract With the object to synthesize polyesters by enzymatic catalysis in organic media, two directions have been investigated: (1) condensation polymerization of linear ω‐hydroxyesters and (2) ring‐opening lactones. The commercially‐available crude porcine pancreatic lipase (PPL), suspended solvents, was preferred enzyme for reactions. In order determine optimal conditions polymerization, bifunctional methyl 6‐hydroxyhexanoate used as a model compound study influence following parameters:...
Collagen cross-linking is altered in many diseases of bone, and enzymatic collagen cross-links are important to bone quality, as evidenced by losses strength after lysyl oxidase inhibition (lathyrism). We hypothesized that also contribute directly fracture toughness. A mouse model lathyrism using subcutaneous injection up 500 mg/kg β-aminopropionitrile (BAPN) was developed characterized (60 animals across 4 dosage groups). Three weeks 150 or 350 BAPN treatment young, growing mice...
Raman spectroscopic markers have been determined for fatigue-related microdamage in bovine bone. Microdamage was induced using a cyclic fatigue loading regime. After loading, the specimens were stained en-bloc with basic fuchsin to facilitate damage visualization and differentiate fatigue-induced from cracks generated during subsequent histological sectioning. Bone tissue examined by light microscopy hyperspectral near-infrared imaging microscopy. Three regions defined-tissue no visible...
Abstract The objective of this study was to address the hypothesis that changes in extracellular pH alter collagen gene expression, synthesis, and alkaline phosphatase activity bone marrow stromal cells (BMSCs). Potential effects on cell function are particular importance for tissue engineering because considerable effort is being placed biodegradable polymers may generate a local acidic microenvironment degradation. Human murine single‐cell suspensions were plated at density 2 × 10 4...
Abstract NMR was used to study the nanostructure of bone tissue. Distance measurements show that first water layer at surface mineral in cortical is structured. This may serve couple organic matrix and play a role deformation. Introduction: The unique mechanical characteristics tissue have not yet been satisfactorily connected exact molecular architecture this complex composite material. Recently developed solid-state nuclear magnetic resonance (NMR) techniques are applied here component...
Understanding the structure and structural changes of bone, a highly heterogeneous material with complex hierarchical architecture, continues to be significant challenge even for high-resolution solid-state NMR spectroscopy. While it is known that dehydration affects mechanical properties bone by decreasing its strength toughness, underlying mechanism at atomic level unknown. Solid-state spectroscopy, controlled dehydration, H/D exchange were used first time reveal an intact piece bovine...
WISP1/CCN4 (hereafter referred to as WISP1), a member of the CCN family, is found in mineralized tissues and produced by osteoblasts their precursors. In this study, Wisp1-deficient (Wisp1−/−) mice were generated. Using dual-energy x-ray absorptiometry, we showed that 3 months, total bone mineral density Wisp1−/− was significantly lower than WT mice. Further investigation micro-computed tomography female had decreased trabecular volume/total volume both male cortical thickness accompanied...
Abstract While obtaining high-resolution structural details from bone is highly important to better understand its mechanical strength and the effects of aging disease on ultrastructure, it has been a major challenge do so with existing biophysical techniques. Though solid-state NMR spectroscopy potential reveal bone, suffers poor spectral resolution sensitivity. Nonetheless, recent developments in magic angle spinning (MAS) technology have made possible spin solid samples up 110 kHz...
The bone diagnostic instrument (BDI) is being developed with the long-term goal of providing a way for researchers and clinicians to measure material properties human in vivo. Such measurements could contribute overall assessment fragility future. Here, we describe an improved BDI, Osteoprobe II™. In II™, probe assembly, which designed penetrate soft tissue, consists reference (a 22 gauge hypodermic needle) test small diameter, sharpened rod) slides through inside probe. assembly inserted...
Abstract Ten episodes of Schönlein‐Henoch purpura (SHP) in 8 patients with familial Mediterranean fever (FMF) were observed. Five developed 3–14 days after penicillin injections, suggesting an etiologic association. FMF and SHP have clear clinical similarities, if the frequency association 2 diseases is indeed high, perhaps a common factor should be sought. An immune complex mechanism might link between these disease entities.
Tissue mechanical properties reflect extracellular matrix composition and organization, as such, their changes can be a signature of disease. Examples such diseases include intervertebral disk degeneration, cancer, atherosclerosis, osteoarthritis, osteoporosis, tooth decay. Here we introduce the tissue diagnostic instrument (TDI), device designed to probe normal diseased soft hard tissues not only in laboratory but also patients. The TDI distinguish between nucleus annulus spinal disks,...