A5047943291- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Physiological and biochemical adaptations
- Metabolism and Genetic Disorders
- Aquaculture Nutrition and Growth
- Muscle metabolism and nutrition
- Genetics, Aging, and Longevity in Model Organisms
- Reproductive biology and impacts on aquatic species
- Cardiovascular Function and Risk Factors
- ATP Synthase and ATPases Research
- Cardiac Ischemia and Reperfusion
- Birth, Development, and Health
- Aquaculture disease management and microbiota
- Pregnancy and preeclampsia studies
- Iron Metabolism and Disorders
- Cell death mechanisms and regulation
- Retinal Diseases and Treatments
- Metabolomics and Mass Spectrometry Studies
- Muscle Physiology and Disorders
- Planarian Biology and Electrostimulation
- Diet and metabolism studies
- Peroxisome Proliferator-Activated Receptors
- Retinal Development and Disorders
- Retinal Imaging and Analysis
- Exercise and Physiological Responses
University of Alberta
2015-2024
Women and Children’s Health Research Institute
2018-2024
Centre Hospitalier Esquirol de Limoges
2021-2023
Innsbruck Medical University
2008-2017
Royal Military College Saint-Jean
2016
Universität Innsbruck
2009-2012
Case Western Reserve University
2009-2011
University School
2011
Université du Québec à Rimouski
1999-2010
United Mitochondrial Disease Foundation
2010
Background Mesenchymal stem cells (MSC) are self-renewing clonal progenitor of nonhematopoietic tissues that exhibit a marked tropism to wounds and tumors. The authors' studies aimed at exploring how local anesthetics would affect MSC biology. Methods Proliferation, colony formation, in vitro wound healing, bone differentiation assays culture-expanded bone-marrow-derived murine were performed the presence increasing concentrations lidocaine, ropivacaine, bupivacaine. Cytotoxicity was...
Endoplasmic reticulum (ER) homeostasis requires molecular regulators that tailor mitochondrial bioenergetics to the needs of protein folding. For instance, calnexin maintains mitochondria metabolism and mitochondria-ER contacts (MERCs) through reactive oxygen species (ROS) from NADPH oxidase 4 (NOX4). However, induction ER stress a quick rewiring adapt new energy needs. This machinery is not characterized. We now show oxidoreductase ERO1⍺ covalently interacts with kinase RNA-like (PERK) upon...
Fuel substrate supply and oxidative phosphorylation are key determinants of muscle performance. Numerous studies mammalian mitochondria carried out (i) with that limits electron flow, (ii) far below physiological temperature. To analyze potentially implicated biases, we studied mitochondrial respiratory control in permeabilized mouse myocardial fibers using high-resolution respirometry. The capacity at 37 °C was nearly two-fold higher when fueled by combinations reconstituting tricarboxylic...
Changes in environmental temperature can pose considerable challenges to animals and shifts thermal habitat have been shown be a major force driving species’ adaptation. These adaptations the focus of research efforts determine physiological or metabolic constraints related reveal phenotypic characters that should adjust. Considering current consensus on climate change, will likely shift questioning whether ectothermic organisms able survive future modifications their niches. Organisms...
Background Intralipid® administration at reperfusion elicits protection against myocardial ischemia-reperfusion injury. However, the underlying mechanisms are not fully understood. Methods Sprague-Dawley rat hearts were exposed to 15 min of ischemia and 30 in absence or presence 1% administered onset reperfusion. In separate experiments, reactive oxygen species (ROS) scavenger N-(2-mercaptopropionyl)-glycine was added either alone with Intralipid®. Left ventricular work activation Akt,...
Background Within the animal kingdom, horses are among most powerful aerobic athletic mammals. Determination of muscle respiratory capacity and control improves our knowledge mitochondrial physiology in high performance general. Methodology/Principal Findings We applied high-resolution respirometry multiple substrate-uncoupler-inhibitor titration protocols to study small (1.0–2.5 mg) permeabilized fibres sampled from triceps brachii healthy horses. Oxidative phosphorylation (OXPHOS) (pmol...
Abstract Breast cancer patients are commonly treated with taxane (e.g. docetaxel) chemotherapy, despite poor outcomes and eventual disease relapse. We previously identified the Bcl-2-associated death promoter (BAD) as a prognostic indicator of good outcome in taxane-treated breast patients. also demonstrated that BAD expression human carcinoma cells generated larger tumors mouse xenograft models. These paradoxical results suggest BAD-expressing differentially sensitive to treatment....
Although evidence that type 2 diabetes mellitus (T2DM) is accompanied by mitochondrial dysfunction in skeletal muscle has been accumulating, a causal link between and the pathogenesis of disease remains unclear. Our study focuses on an early stage to determine whether contributes development T2DM. The fructose-fed (FF) rat was used as animal model Mitochondrial respiration acylcarnitine species were measured oxidative (soleus) glycolytic [extensor digitorum longus (EDL)] muscle. FF rats...
Early stage localized prostate cancer (PCa) has an excellent prognosis; however, patient survival drops dramatically when PCa metastasizes. The molecular mechanisms underlying metastasis are complex and remain unclear. Here, we examine the role of a new member fatty acid-binding protein (FABP) family, FABP12, in progression. FABP12 is preferentially amplified and/or overexpressed metastatic compared to primary tumors from both patients xenograft animal models. We show that concurrently...
Concern about the overexploitation of wild aquatic resources, slow recovery groundfish fisheries and need to encourage diversification mariculture industry province Quebec (Canada) all provided strong incentive explore potential a wide selection marine anadromous fish species for cold-water mariculture. Starting from list over 45 indigenous commercial interest, biotechnical review was initiated. Technical sheets each were produced aquaculture-based criteria covering three aquaculture...
We measured the loss of cardiac mitochondrial function related to aging in males three rat strains presenting with different longevity and phenotypes: Fischer 344 (F344), Brown Norway (BN), hybrid F344×BN. The F344 has a short life span ∼45% decrease coupled oxidation permeabilized fibers from old rats compared young rats. Citrate synthase activity (mitochondrial content) did not change significantly aging. BN live longer have 15%–18% respiration aged differences are significant. In hybrids,...
Prenatal iron deficiency alters fetal developmental trajectories, which results in persistent changes organ function. Here, we studied the effects of prenatal on kidney and liver mitochondrial Pregnant Sprague-Dawley rats were fed partially or fully iron-restricted diets to induce a state moderate severe alongside iron-replete control rats. We assessed function via high-resolution respirometry reactive oxygen species generation fluorescence microscopy gestational d 21. Hemoglobin levels...
Perinatal iron deficiency (ID) alters developmental trajectories of offspring, predisposing them to cardiovascular dysfunction in later life. The mechanisms underlying this long-term programming renal function have not been defined. We hypothesized perinatal ID causes hypertension and kidney metabolic morphology a sex-dependent manner adult offspring. Furthermore, we these effects are exacerbated by chronic consumption high salt diet.Pregnant Sprague Dawley rats were fed either an...
Despite the fact that skeletal muscle insulin resistance is hallmark of type-2 diabetes mellitus (T2DM), inflexibility in substrate energy metabolism has been observed other tissues such as liver, adipose tissue, and heart. In heart, structural functional changes ultimately lead to diabetic cardiomyopathy. However, little known about early biochemical cause cardiac metabolic dysregulation dysfunction. We used a dietary model fructose-induced T2DM (10% fructose drinking water for 6 weeks)...
The Bcl-2-associated death promoter BAD is a prognostic indicator for good clinical outcome of breast cancer patients; however, whether affects biology unknown. Here we showed that increased cell growth in cells through two distinct mechanisms. Phosphorylation at S118 S99 phosphorylation, 14-3-3 binding and AKT activation to promote survival. Through second, more prominent pathway, stimulated mitochondrial oxygen consumption novel manner was downstream substrate entry into the mitochondria....
Pregnancy complications associated with prenatal hypoxia lead to increased placental oxidative stress. Previous studies suggest that can reduce mitochondrial respiratory capacity and fusion, which could dysfunction impaired fetal development. We developed a placenta-targeted treatment strategy using antioxidant, MitoQ, encapsulated into nanoparticles (nMitoQ) stress (indirectly) improve outcomes. hypothesized that, in rat model of hypoxia, nMitoQ improves function promotes fusion both male...