A5011203970- Glycosylation and Glycoproteins Research
- Quantum Dots Synthesis And Properties
- Monoclonal and Polyclonal Antibodies Research
- Carbohydrate Chemistry and Synthesis
- Carbon and Quantum Dots Applications
- Advanced biosensing and bioanalysis techniques
- Proteoglycans and glycosaminoglycans research
- Enzyme Structure and Function
- Chemical Synthesis and Analysis
- Analytical Chemistry and Chromatography
- Galectins and Cancer Biology
- Polysaccharides and Plant Cell Walls
- Nanocluster Synthesis and Applications
- Protein Structure and Dynamics
- Virus-based gene therapy research
- Protein purification and stability
- Advanced Drug Delivery Systems
- Advanced Biosensing Techniques and Applications
- Studies on Chitinases and Chitosanases
- Machine Learning in Bioinformatics
- Biopolymer Synthesis and Applications
- Hereditary Neurological Disorders
- Graphene and Nanomaterials Applications
- Enzyme Production and Characterization
- Ubiquitin and proteasome pathways
Komatsu (Japan)
2014-2025
Tohoku Medical and Pharmaceutical University
2014-2025
Gifu Prefectural Research Institute for Forests
2024
Gifu University
2024
Osaka Prefecture University
2019-2021
Tohoku University
2011-2019
National Center for Global Health and Medicine
2011-2013
Center for Global Health
2012
Japan Association for the Advancement of Medical Equipment
2008-2010
Saitama International Medical Center
2004-2010
Semiconductor quantum dots (QDs) hold some advantages over conventional organic fluorescent dyes. Due to these advantages, they are becoming increasingly popular in the field of bioimaging. However, recent work suggests that cadmium based QDs affect cellular activity. As a substitute for QDs, we have developed photoluminescent stable silicon (Si-QDs) with passive-oxidation technique. Si-QDs (size: 6.5 ± 1.5 nm) emit green light, and been used as biological labels living cell imaging. In...
Quantum dots (QDs) have been applied to a wide range of biological studies by taking advantage their fluorescence properties. There is almost no method trace small molecules including medicine. Here, we used QDs for fluorescent tracers medicine and analyzed kinetics dynamics. We conjugated with captopril, anti-hypertensive medicine, an exchange reaction while retaining the medicinal investigated effect QD-conjugated captopril (QD-cap) in vitro vivo. also evaluated concentration distribution...
Quantum dots (QDs) are well known for their potential application in biosensing, ex vivo live-cell imaging and animal targeting. The brain is a challenging organ drug delivery, because the blood barrier (BBB) functions as gatekeeper guarding body from exogenous substances. Here, we evaluated distribution of bioconjugated QDs, i.e., captopril-conjugated QDs (QDs-cap) following intraperitoneal injection into male ICR mice model system determining tissue localization employing ICP-MS confocal...
Various computational methods have been developed to predict the pathogenicity of missense variants, which is crucial for diagnosing rare diseases. Recently, we introduced VarMeter, a diagnostic tool predicting variant based on normalized solvent-accessible surface area (nSASA) and mutation energy calculated from AlphaFold 3D models, validated it arylsulfatase L. To evaluate broader applicability VarMeter enhance its predictive accuracy, here analyzed 296 pathogenic 240 benign variants...
Immunological diagnostic methods have been widely performed and showed high performance in molecular cellular biology, imaging, medical diagnostics. We developed novel for the fluorescent labeling of several antibodies coupled with nanocrystal QDs. In this study we demonstrated that two bacterial toxins, diphtheria toxin tetanus toxin, were detected simultaneously same view field a cover slip by using directly QD-conjugated antibodies. succeeded detecting toxins counting luminescent spots on...
Many natural mutants of the drug metabolizing enzyme cytochrome P450 (CYP) 2D6 have been reported. Because enzymatic activities many are different from that wild type, genetic polymorphism CYP2D6 plays an important role in metabolism. In this study, molecular dynamics simulations type and CYP2D6, CYP2D6.1, 2, 10, 14A, 51, 62 were performed, predictions static dynamic structures within them conducted. mutant CYP2D6.10, 61, properties F-G loop, which is one components active site access...
Ribitol (C5H12O5) is an acyclic sugar alcohol that was recently identified in O-mannose glycan on mammalian α-dystroglycan. The conformation and dynamics of alcohols such as ribitol are dependent the stereochemistry hydroxyl groups; however, not fully understood. To gain insights into alcohols, we carried out comparative analyses ribitol, d-arabitol xylitol by a crystal structure database search, solution NMR analysis molecular (MD) simulations. structures showed limited number...
Aspartic acid (Asp) residues in peptides and proteins are prone to isomerization the β-form racemization via a five-membered succinimide intermediate. These nonenzymatic reactions have relevance aging age-related diseases. In this paper, we report three water molecule-assisted, six-step mechanism for formation of from Asp found by density functional theory calculations. The first two steps constitute stepwise iminolization C-terminal amide group. This involves quintuple proton transfer along...
Silicon quantum dots (Si-QDs) have great potential for biomedical applications, including their use as biological fluorescent markers and carriers drug delivery systems. Biologically inert Si-QDs are less toxic than conventional cadmium-based QDs, can modify the surface of Si-QD with covalent bond. We synthesized water-soluble alminoprofen-conjugated (Ap-Si). Alminoprofen is a non-steroid anti-inflammatory (NSAID) used an analgesic rheumatism. Our results showed that “silicon drug” control...
In proteins and peptides, d-aspartic acid (d-Asp) d-β-Asp residues can be spontaneously formed via racemization of the succinimide intermediate from l-Asp l-asparagine (l-Asn) residues. These biologically uncommon amino are known to have relevance aging pathologies. Although nonenzymatic, will not occur without a catalyst at room or biological temperature. present study, we computationally investigated mechanism catalyzed by dihydrogen phosphate ion, H₂PO₄-, B3LYP/6-31+G(d,p) density...
Abstract N -Glycosylation is a common post-translational modification, and the number of GlcNAc branches in -glycans impacts glycoprotein functions. -Acetylglucosaminyltransferase-IVa (GnT-IVa, also designated as MGAT4A) forms β1-4 branch on α1-3 mannose arm -glycans. Downregulation or loss GnT-IVa causes diabetic phenotypes by dysregulating glucose transporter-2 pancreatic β-cells. Despite physiological importance GnT-IVa, its structure catalytic mechanism are poorly understood. Here, we...
Fluorescent nanocrystal quantum dots (QDs) are widely used as novel tools in various biological fields including cellular biology, molecular and even basic clinical medical fields, due to their far brighter photoemission photostability. Although many amounts of studies, vivo experiments, were circumstantially investigated, there is no informative report that investigates whether the QDs affect mammalian immune system. This study investigated response behavior vitro vivo. The by both...
Vitreous is transparent tissue located between the lens and retina of eye, thus, difficult to look at by even ophthalmological microscope. But vitreous connected with some sight-threatening eye diseases, for example, retinal detachment, macular hole, epi-retinal membrane, so forth. Quantum dots (QDs) have been applied a wide range biological studies taking advantage their fluorescence properties. We established novel technique aqueous colloidal QD (ACQD) as lesion detector. When compared...
The Asn-Gly-Arg (NGR) motif and its deamidation product isoAsp-Gly-Arg (isoDGR) have recently attracted considerable attention as tumor-targeting ligands. Because an NGR-containing peptide the corresponding isoDGR-containing target different receptors, spontaneous NGR can be used in dual targeting strategies. It is well known that Asn proceeds via a succinimide derivative. In present study, we computationally investigated mechanism of formation from cyclic peptide, c[CH₂CO-NGRC]-NH₂, which...
Succinimide formation from aspartic acid (Asp) residues is a concern in the formulation of protein drugs. Based on density functional theory calculations using Ace-Asp-Nme (Ace = acetyl, Nme NHMe) as model compound, we propose possibility that acetic (AA), which often used drug for mildly acidic buffer solutions, catalyzes succinimide Asp by acting proton-transfer mediator. The proposed mechanism comprises two steps: cyclization (intramolecular addition) to form gem-diol tetrahedral...