A5027664329- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Galectins and Cancer Biology
- Monoclonal and Polyclonal Antibodies Research
- Proteoglycans and glycosaminoglycans research
- Liver Disease Diagnosis and Treatment
- Hepatitis B Virus Studies
- Enzyme Production and Characterization
- Hepatitis C virus research
- Neuroinflammation and Neurodegeneration Mechanisms
- Peptidase Inhibition and Analysis
- Cell Adhesion Molecules Research
- RNA and protein synthesis mechanisms
- Advanced Proteomics Techniques and Applications
- Cancer Research and Treatments
- Escherichia coli research studies
- Genetics, Bioinformatics, and Biomedical Research
- Immune Response and Inflammation
- Machine Learning in Bioinformatics
- Digestive system and related health
- Endoplasmic Reticulum Stress and Disease
- Immune Cell Function and Interaction
- Neurogenesis and neuroplasticity mechanisms
- Lung Cancer Research Studies
- Genetics, Aging, and Longevity in Model Organisms
Soka University
1998-2025
National Institute of Advanced Industrial Science and Technology
2014-2024
Hepatitis B Foundation
2015
Drug Discovery Laboratory (Norway)
2015
University of Tsukuba
2005
Noguchi Institute
2005
New Energy and Industrial Technology Development Organization
2002-2004
Fujirebio (Japan)
2003
Jichi Medical University
2003
The University of Tokyo
2001
The Wisteria floribunda agglutinin-positive human Mac-2-binding protein (WFA+-M2BP) was recently shown to be a liver fibrosis glycobiomarker with unique fibrosis-related glycoalteration. We evaluated the ability of WFA+-M2BP predict development hepatocellular carcinoma (HCC) in patients who were infected hepatitis C virus (HCV). A total 707 had been admitted our hospital chronic HCV infection without other potential risk factors determine HCC; included age, sex, viral load, genotypes, stage,...
The glycome represents the total set of glycans expressed in a cell. has been assumed to vary between cell types, stages development and differentiation, during malignant transformation. Analysis provides basis for understanding functions these cellular processes. Recently, technique called lectin microarray was developed rapid profiling glycosylation, although its use mainly restricted glycoproteins lysates, thus unable profile intact surface glycans. Here we report simple sensitive...
The core 3 structure of the O-glycan, GlcNAcβ1–3GalNAcα1-serine/threonine, an important precursor in biosynthesis mucin-type glycoproteins, is synthesized by UDP-N-acetylglucosamine:GalNAc-peptide β1,3-N- acetylglucosaminyltransferase (β3Gn-T; synthase). restricted its occurrence to mucins from specific tissues such as stomach, small intestine, and colon. A partial sequence encoding a novel member human β3Gn-T family was found one data bases. We cloned complementary DNA this gene named it...
The sialyl Lewis a antigen is well known tumor marker, CA19-9, which frequently elevated in the serum gastrointestinal and pancreatic cancers. UDP-galactose:<i>N</i>-acetylglucosamine β1,3-galactosyltransferase(s) (β3Gal-Ts) are required for synthesis of epitope. In present study, novel β3Gal-T, named β3Gal-T5, was isolated from Colo205 cDNA library using degenerate primer strategy based on amino acid sequences four human <i>β3Gal-T</i> genes cloned to date. Transfection experiments...
The core 3 structure of the O -glycan, GlcNAcβ1-3GalNAcα1-Ser/Thr, an important precursor in biosynthesis mucin-type glycoproteins, is synthesized by β1,3- N -acetylglucosaminyltransferase 6 (β3Gn-T6; synthase). We generated anti-β3Gn-T6 mAb (G8-144 mAb) and performed immunohistochemical analyses. In normal stomach colon, β3Gn-T6 was strongly expressed Golgi region epithelia. contrast, its expression markedly down-regulated gastric colorectal carcinomas. Tissue specimens from a familial...
To date, 10 members of the UDP-<i>N</i>-acetyl-α-d-galactosamine:polypeptide<i>N</i>-acetylgalactosaminyltransferase (pp-GalNAc-T) family have been cloned and analyzed in human. In this study, we a novel human pp-GalNAc-T from an NT2 cell cDNA library, named it pp-GalNAc-T13. amino acid sequences, pp-GalNAc-T13 was highly homologous, showing 84.3% identity, to pp-GalNAc-T1. Real time PCR analysis revealed <i>pp-GalNAc-T13</i> be restrictively expressed brain present at very low or...
beta1,3-N-acetylglucosaminyltransferase 2 (beta3GnT2) is a polylactosamine synthase that synthesizes backbone structure of carbohydrate structures onto glycoproteins. Here we generated beta3GnT2-deficient (beta3GnT2(-/-)) mice and showed on N-glycans was markedly reduced in their immunological tissues. In WT mice, present CD28 CD19, both known immune costimulatory molecules. However, levels these molecules were beta3GnT2(-/-) mice. T cells lacking more sensitive to the induction...
Measurement of Wisteria floribunda agglutinin-positive human Mac-2 binding protein (WFA+-M2BP) in serum was recently shown to be a noninvasive method assess liver fibrosis. The aim this study evaluate the utility WFA+-M2BP values predict development hepatocellular carcinoma (HCC) patients who achieved sustained virological response (SVR) by interferon treatment. For purpose, we retrospectively analyzed 238 with SVR were treated our department. Serum measured at pre-treatment (pre-Tx),...
We have isolated three types of cDNAs encoding novel β1,3-<i>N</i>-acetylglucosaminyltransferases (designated β3Gn-T2, -T3, and -T4) from human gastric mucosa the neuroblastoma cell line SK-N-MC. These enzymes are predicted to be type 2 transmembrane proteins 397, 372, 378 amino acids, respectively. They share motifs conserved among members β1,3-galactosyltransferase family a β1,3-<i>N</i>-acetylglucosaminyltransferase β3Gn-T1), but show no structural similarity another (iGnT). Each...
A new member of the UDP-N-acetylglucosamine:β-galactose β1,3-N-acetylglucosaminyltransferase (β3Gn-T) family having β3Gn-T motifs was cloned from rat and human cDNA libraries named β3Gn-T5 based on its position in a phylogenetic tree. We concluded that is most feasible candidate for lactotriaosylceramide (Lc3Cer) synthase, an important enzyme which plays key role synthesis lacto- or neolacto-series carbohydrate chains glycolipids. exhibited strong activity to transfer GlcNAc glycolipid...
By a tblastn search with β1,4-galactosyltransferases as query sequences, we found an expressed sequence tag that showed similarity in β1,4-glycosyltransferase motifs. The full-length complementary DNA was obtained by method of 5′-rapid amplification ends. predicted open reading frame encodes typical type II membrane protein comprising 543 amino acids, the which highly homologous to chondroitin sulfate <i>N</i>-acetylgalactosaminyltransferase (CSGalNAcT-1), and designated this novel enzyme...
The 3-fucosyl-N-acetyllactosamine (Lewis x, CD15, SSEA-1) carbohydrate epitope is widely distributed in many tissues and developmentally expressed some rodent human tissues, i.e. brain lung, mouse early embryo. In such the Lewis x considered to be involved cell-cell interactions. We isolated a novel alpha1,3-fucosyltransferase gene, named mFuc-TIX, from an adult cDNA library using expression cloning method. On flow cytometric analysis, Namalwa cells transfected stably with mFuc-TIX gene...
A novel member of the human UDP‐ N ‐acetyl‐ D ‐galactosamine:polypeptide ‐acetylgalactosaminyltransferase (pp‐GalNAc‐T) gene family was cloned as a homolog pp‐GalNAc‐T7, and designated pp‐GalNAc‐T10. pp‐GalNAc‐T10 transcript found in small intestine, stomach, pancreas, ovary, thyroid gland spleen. In polypeptide GalNAc‐transfer assay, recombinant transferred GalNAc onto panel mucin‐derived peptide substrates. Furthermore, demonstrated strong transferase activity with glycopeptide
We found a novel human glycosyltransferase gene carrying hypothetical β1,4-glycosyltransferase motif during BLAST search, and we cloned its full-length open reading frame by using the 5′-rapid amplification of cDNA ends method. It encodes type II transmembrane protein 999 amino acids with homology to chondroitin sulfate synthase in C-terminal region (GenBank™ accession number AB089940). Its putative orthologous was also mouse (accession AB114826). The truncated form enzyme expressed HEK293T...
A new member of the UDP‐ N ‐acetylglucosamine: β‐galactose β1,3‐ ‐acetylglucosaminyltransferase (β3Gn‐T) family having β3‐glycosyltransferase motifs was identified using an in silico method. This novel β3Gn‐T cloned from a human colon cancer cell line and named β3Gn‐T8 based on its position phylogenetic tree enzymatic activity. transfers GlcNAc to non‐reducing terminus Galβ1–4GlcNAc tetraantennary ‐glycan vitro. HCT15 cells transfected with cDNA showed increase reactivity both LEA PHA‐L4...
Wisteria floribunda agglutinin (WFA)-positive human Mac-2-binding protein (WFA(+) -M2BP) is a new glycol marker related to liver fibrosis. The aim of the present study was evaluate WFA(+) -M2BP as predictor hepatocellular carcinoma (HCC) development in patients with chronic hepatitis C.This case-control included 14 C who developed HCC and 52controls, matched for age, gender, fibrosis stage. measured at biopsy follow-up. Time zero set date biopsy.WFA(+) increased stepwise progression (p <...
Abstract The absence of highly specific markers for malignant mesothelioma (MM) has served an obstacle its diagnosis and development molecular-targeting therapy against MM. Here, we show that a novel mucin-like membrane protein, sialylated protein HEG homolog 1 (HEG1), is marker A monoclonal antibody HEG1, SKM9-2, can detect even sarcomatoid desmoplastic specificity sensitivity SKM9-2 to MM reached 99% 92%, respectively; this did not react with normal tissues. This accurate discrimination by...
The hinge region of human immunoglobulin A1 (*IgA1) possesses multiple O-glycans, which synthesis is initiated by the addition GalNAc to serine or threonine through activity UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferases (pp-GalNAc-Ts). We found that six pp-GalNAc-Ts, pp-GalNAc-T1, -T2, -T3, -T4, -T6, and -T9, were expressed in B cells, IgA-bearing NCI-H929 IgA myeloma cells. pp-GalNAc-T activities these enzymes for a synthetic peptide, has nine possible...
Chondroitin sulfate is found in a variety of tissues as proteoglycans and consists repeating disaccharide units N-acetylgalactosamine glucuronic acid residues with at various places. We novel human gene (GenBank™ accession number AB086063) that possesses sequence homologous the chondroitin glucuronyltransferase which we recently cloned characterized. The full-length open reading frame encodes typical type II membrane protein comprising 775 amino acids. had domain containing...
We found a novel glycosyltransferase gene having hypothetical β1,4-galactosyltransferase motif (GenBank<sup>TM</sup> accession number AB081516) by BLAST search and cloned its full-length open reading frame using the 5′-rapid amplification of cDNA ends method. The truncated form was expressed in insect cells as soluble enzyme. It transferred<i>N</i>-acetylgalactosamine, not galactose, to<i>para</i>-nitrophenyl-β-glucuronic acid. The<i>N</i>-acetylgalactosamine-glucuronic acid linkage has been...
Protein O-linked fucosylation is an unusual glycosylation associated with many important biological functions such as Notch signaling. Two pathways synthesizing O-fucosylglycans have been reported on cystein-knotted proteins, that is, epidermal growth factor-like (EGF-like) domains and thrombospondin Type 1 repeat (TSR) domains. We report here the molecular cloning characterization of a novel β1,3-glucosyltransferase (β3Glc-T) synthesizes Glcβ1,3Fucα- structure TSR domain. found...