Wisteria floribunda agglutinin positive human Mac-2-binding protein (WFA(+)-hM2BP) was recently validated as a liver fibrosis glycobiomarker with fully automated lectin-antibody sandwich immunoassay. In this study, we supplied recombinant WFA(+)-hM2BP the standard glycoprotein and overlaid antibody to enhance robustness of quantification.The optimum conditions for producing were selected by cell glycome analysis based on lectin microarray. Interlot variability determined using an...

Despite the progress made in understanding glyco-alterations of specific glycoproteins such as α1-acid glycoprotein (AGP) associated with liver fibrosis, there has been no useful diagnostic assay a lectin recognizing fibrosis-specific alteration and an antibody against core protein. We therefore developed compatible multiple lectin-antibody sandwich immunoassay on basis results obtained by microarray analysis for monitoring fibrosis.AGP-enriched fractions derived from 0.5-μL sera 125...

Protein O-linked fucosylation is an unusual glycosylation associated with many important biological functions such as Notch signaling. Two pathways synthesizing O-fucosylglycans have been reported on cystein-knotted proteins, that is, epidermal growth factor-like (EGF-like) domains and thrombospondin Type 1 repeat (TSR) domains. We report here the molecular cloning characterization of a novel β1,3-glucosyltransferase (β3Glc-T) synthesizes Glcβ1,3Fucα- structure TSR domain. found...

Glycan alterations of proteins, a common feature cancer cells, are associated with carcinogenesis, invasion and metastasis. Glycomics, the study glycans glycan-binding proteins in various biological systems, is an emerging field postgenome postproteomics era. However, systematic robust strategies for glycomics still not fully established because structural analysis glycans, which comprise different patterns branching, possible linkage positions as well monomer anomericity, technically...

In this paper, a simple and efficient numerical method to solve for the dynamic interaction of high-speed train railway structure during an earthquake is given. The motion modeled in multibody dynamics with nonlinear springs dampers used connect components. An mechanical model contact between wheel rail presented. various finite elements. A spring element based on trilinear elastic-plastic material given concrete earthquake. substructure where runs repeatedly has been devised obtain...

We previously proposed a high-throughput strategy to discover serological biomarker candidates of cancer. This focuses on series candidate glycoproteins that are specifically expressed in the original tissues (cells) target cancer and carry glycan structures associated with carcinogenesis [Narimatsu, H., et al. FEBS J.2010, 277(1), 95-105]. Here, we examined effectiveness our identifying biomarkers assess progression liver fibrosis for early detection hepatocellular carcinoma (HCC). On basis...

Epithelial ovarian cancer (EOC) is often asymptomatic and thus diagnosed at advanced stages with a poor prognosis. False-negative results for the conventional marker CA125 frequently occur in cases of clear cell carcinoma (CCC), type EOC; therefore, it necessary to develop biomarkers greater sensitivity. We previously reported strategy discover glycobiomarker candidates by combined lectin microarray IGOT–LC/MS analysis. have now optimized this discovering EOC biomarkers. Glycopeptides...

The importance of diagnosis and therapies for liver cirrhosis (LC) is indisputable. Thus, a reliable method monitoring the progression fibrosis resultant LC urgently needed. Previously, using lectin-assisted glycoproteomic method, we identified 26 serum glycoproteins as promising glycobiomarker candidates diseases. In this study, colony stimulating factor 1 receptor (CSF1R) marker candidate then established Wisteria floribunda agglutinin (WFA)-reactive CSF1R (WFA(+)-CSF1R) novel possible by...

Abstract The extent of liver fibrosis predicts prognosis and is important for determining treatment strategies chronic hepatitis. During the progression, serum levels Mac2 binding protein (M2BP) increase N-glycan structure changes to enable Wisteria floribunda agglutinin (WFA) lectin. As a novel diagnostic marker, glycosylation isomer M2BP (M2BPGi) has been developed. However, its glycan structures recognized by WFA are unclear. In this study, we analyzed site-specific using Glyco-RIDGE...

Serum hepatitis B surface antigen (HBsAg) is a component of both virus (HBV) virions and non-infectious subviral particles (SVPs). Recently, O-glycosylation the PreS2 domain middle HBsAg protein has been identified as distinct characteristic genotype C HBV versus SVPs. This study aimed to evaluate serum O-glycosylated levels in patients with chronic (CHB) treated nucleos(t)ide analogs (NAs).Forty-seven treatment-naïve CHB were retrospectively enrolled. at baseline after 48 weeks NA therapy...

A simple and efficient numerical model to solve the dynamic interaction of a high-speed train railway structure including derailment post-derailment during an earthquake is given. Mechanical express contact-impact behaviours between wheel rail before track after discussed. contact based on multipoint springs surface An element behaviour car body also given where stiffness described as nonlinear function displacement. The motion with dampers modelled in multibody dynamics. various finite...

Aims: The aim of this work was to develop a highly selective method detecting sulphate-reducing bacteria (SRB) in crude oil. Methods: A pair PCR primers designed based on an alignment the nucleotide sequence 16S rRNA genes from Desulfovibrionaceae family. DNA extraction oil performed by using zirconia beads and stool kit. Results: specifically detected Desulfovibrio Desulfomicrobium sediment sample. When nucleic acids extracted directly were used for PCR, 16SrRNAgenes Thermodesulforhabdus...

Wisteria floribunda agglutinin (WFA)-reactive ceruloplasmin (CP) is a candidate marker for ovarian clear cell carcinoma (CCC) reported in our previous paper. Herein, new measurement system was developed to investigate its potential as serum CCC. Site-specific glycome analysis using liquid chromatography/mass spectrometry showed that WFA-CP from CCC binds WFA via the GalNAcβ1,4GlcNAc (LDN) structure. We used mutant recombinant (rWFA), which has high specificity LDN structure, instead of...

Plasminogen activator inhibitor-1 (PAI-1) is a key regulator of the fibrinolytic system (1). The continuously high PAI-1 concentrations make fibrins resistant to dissolution by tissue-type plasminogen (tPA), which leads multiorgan dysfunction and bad prognosis in patients (2). Several methods have been developed for measuring functional activity plasma samples based on principle adding specified amount tPA excess residual after short incubation period (3). These methods, however, are neither...

A computational model to solve for the dynamic interaction of a high-speed train and railway structure including derailment during an earthquake is given.An efficient mechanical express contactimpact behaviours between wheel rail before derailment, track after car body presented.The motion with nonlinear springs dampers modelled in multibody dynamics.The various finite elements.The combined response obtained by solving equations motions modal coordinates subjected structure.Numerical...

A combined multibody and finite element approach is given to solve the dynamic interaction of a Shinkansen train (high-speed in Japan) railway structure including post-derailment during an earthquake effectively. The motion expressed dynamics. Efficient mechanical models express interactions between wheel track are given. Rail elements dynamics FEM contact problems long components modeled with various rail elements. computer program has been developed for analysis post derailment earthquake....

既設鉄道ラーメン高架橋の高欄は,かぶり不足や発錆限界値を超える初期塩化物イオン量により中性化と内的塩害による複合劣化を起こし変状が顕在化している場合が多く見られる。場所打ちRC高欄の変状は,側道や交差道路上において第三者に被害を及ぼす可能性があるため,的確な対処が求められている。本報告では,経年25年を超える場所打ち高欄を対象として,メッシュ分割法により高欄の変状率を定量化するとともに,鉄筋のかぶり,中性化深さ,初期塩化物イオン濃度等のばらつきに関する統計データを収集した。またこれらのデータに基づき,当該地区の高欄の変状率の支配的劣化要因が鉄筋かぶり不足であることを明らかにした。これらの調査結果に基づきモンテカルロシミュレーションによる変状予測を行い,今後10年で,はく離,はく落箇所は,全体の10~30%増加することを示した。また同シミュレーションより,鉄筋の腐食速度が,鉄道構造物等維持管理標準・同解説(コンクリート構造物編)に示された値の50%以下となる場合が多いことが分かった。

A wheel-rail contact method for analyzing the train-structure nonlinear interaction that takes into account wheel and rail geometry is proposed in this article. The presented formulation divided three main parts: 1) geometric problem consisting of detection point calculation all variables needed determining forces; 2) normal which forces are determined based on Hertz theory; 3) tangential calculated using rolling friction laws by Kalker. Most existing methods treat directions as external...

In this paper, a simple and efficient numerical method to solve for the dynamic interaction of Shinkansen train (high-speed in Japan) railway structure during an earthquake is given. The motion modeled multibody dynamics with nonlinear springs dampers used connect components. An mechanical model contact between wheel rail presented. various finite elements. A three-dimensional spring element based on trilinear elastic-plastic material given concrete earthquake. loop has been devised obtain...

The conventional seismic standard of Japan specifies that the inertia force trains is modeled as a uniformly-distributed load which has upper limit 30% railway vehicle weight assuming vehicles produce damping effects or do not always oscillate in same phase structures.However, effect been specifically identified and unreliable because actual have complicated vibration system.The object this paper to evaluate dynamic on response structures, develop reasonable modeling method design.As result...