A5029345929- Glycosylation and Glycoproteins Research
- Natural Language Processing Techniques
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Advanced Proteomics Techniques and Applications
- Topic Modeling
- Galectins and Cancer Biology
- Carbohydrate Chemistry and Synthesis
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Enzyme Production and Characterization
- Enzyme Structure and Function
- Alzheimer's disease research and treatments
- Speech and dialogue systems
- Folate and B Vitamins Research
- Biochemical and Molecular Research
- Ubiquitin and proteasome pathways
- Lipid Membrane Structure and Behavior
- SARS-CoV-2 and COVID-19 Research
- Genetics, Aging, and Longevity in Model Organisms
- Bacterial Genetics and Biotechnology
- Metabolism and Genetic Disorders
- Phytase and its Applications
Nagoya University
2023-2025
National Institute of Advanced Industrial Science and Technology
2015-2024
Drug Discovery Laboratory (Norway)
2015
Shizuoka University
2006-2014
Utsunomiya University
2013
Tokyo Metropolitan University
2000-2012
Thomas Jefferson University
1982-2008
Hitachi (Japan)
1984-2005
Tokyo University of Agriculture and Technology
2002-2005
Pioneer (Japan)
2002-2005
Increasing evidence suggests that α-synuclein is a common pathogenic molecule in several neurodegenerative diseases, particularly Parkinson's disease. To understand pathology, we investigated molecules interact with human and rat brains identified tubulin as an binding/associated protein. Tubulin co-localized Lewy bodies other α-synuclein-positive pathological structures. initiated promoted fibril formation under physiological conditions vitro. These findings suggest interaction between...
This paper proposes a two-phase example-based machine translation methodology which develops templates from examples and then translates using template matching. method improves quality facilitates customization of systems. focuses on the automatic learning templates. A is bilingual pair sentences in corresponding units (words pharases) are coupled replaced with variables. Correspondence between determined by suing dictionary analyzing syntactic structure sentences. Syntactic ambiguity...
We have cloned and sequenced the translocated c-myc gene from Burkitt's lymphoma CA46 cell line that carries a reciprocal translocation between chromosomes 8 14. The breakpoint lies within first intron of c-myc, so noncoding exon remains on 8q- chromosome. second third coding exons are to 14q+ chromosome into switch region C-alpha 1. orientation with relationship alpha 1 is 5' 5', directions transcription in opposite orientation. DNA sequencing studies predict five changes amino acid...
Protein glycosylation is a common post-translational modification that plays important roles in terms of protein function. However, analyzing the relationship between and function remains technically challenging. This problem arises from fact attached glycans possess diverse heterogeneous structures. We believe first step to elucidate glycan systematically determine status under physiological conditions. Such studies involve differences structure on cell type (tissue), sex, age, as well...
A proteome of a model organism, Caenorhabditis elegans, was analyzed by an integrated liquid chromatography (LC)-based protein identification system, which constructed microscale two-dimensional (2DLC) coupled with electrospray ionization (ESI) tandem mass spectrometry (MS/MS) on high-resolution hybrid spectrometer automated data analysis system. Soluble and insoluble fractions were prepared from mixed growth phase culture the worm C. digested trypsin, fractionated separately 2DLC The...
ST6GalNAcI is a sialyltransferase responsible for the synthesis of sialyl Tn (sTn) antigen which expressed in variety adenocarcinomas including gastric cancer especially advanced cases, but roles and sTn progression are largely unknown. We generated sTn-expressing human cells by ectopic expression to evaluate metastatic ability these prognostic effect mouse model, identified carrier proteins gain insight into function progression. A green fluorescent protein-tagged cell line was transfected...
PC63 spectrophotometric titration with p-mercuribenzoate, in a Beckman model DKl instrument.In all four cases, the number of free -SH groups observed was 2 per molecule (68,000) to within &5%.We have also studied rates denaturation, by acid, alkali, and heat native hemoglobin, three modified forms.The results, which will be reported indetail later, show no striking differences.Likewise, curves forms indication unmasking any substantial titratable various digestion products.Observations on...
The significance of glycomic profiling has been highlighted by recent findings that structural changes glycans are observed in many diseases, including cancer. Therefore, the whole body (glycome mapping) under different physiopathological states may contribute to discovery reliable biomarkers with disease-specific alterations. To achieve this, standardization high-throughput and in-depth analysis tissue glycome mapping is needed. However, this a great challenge due lack analytical...
The 14-3-3 protein family plays a role in wide variety of cell signaling processes including monoamine synthesis, exocytosis, and cycle regulation, but the structural requirements for activity this are not known. We have previously shown that binds with activates phosphorylated tryptophan hydroxylase (TPH, rate-limiting enzyme biosynthesis neurotransmitter serotonin) proposed might be mediated through COOH-terminal acidic region molecules. In report we demonstrate, using series truncation...
Horizontally transferred genes are believed to play a critical role in the divergence of bacterial strains from common ancestor, but whether all these express functional proteins cell remains unknown. Here, we used an integrated LC-based protein identification technology analyze proteome Escherichia coli strain K12 (JM109) and identified 1,480 expressed proteins, which equivalent ∼35% total open reading frames predicted genome. This subset contained with cellular abundance several dozens...
Laser ablation MC-ICP-MS allows in situ strontium isotope data to be obtained for incrementally formed bioapatites such as enamel with extremely high spatial resolution. Here, we provide a large-scale application of the method comparing ...Understanding mobility and landscape use is important reconstructing subsistence behavior, range, group size, it may contribute our understanding phenomena dynamics biological cultural interactions between distinct ...
We previously proposed a high-throughput strategy to discover serological biomarker candidates of cancer. This focuses on series candidate glycoproteins that are specifically expressed in the original tissues (cells) target cancer and carry glycan structures associated with carcinogenesis [Narimatsu, H., et al. FEBS J.2010, 277(1), 95-105]. Here, we examined effectiveness our identifying biomarkers assess progression liver fibrosis for early detection hepatocellular carcinoma (HCC). On basis...
The coronavirus disease (COVID-19) pandemic, caused by severe acute respiratory syndrome 2 (SARS-CoV-2), has impacted public health globally. As the glycosylation of viral envelope glycoproteins is strongly associated with their immunogenicity, intensive studies have been conducted on glycans glycoprotein SARS-CoV-2, spike (S) protein. Here, we glycoproteomic analyses SARS-CoV-2 S protein ancestral and γ-variant strains using a combinatorial approach two different technologies: mass...
The Human Glycome Atlas (HGA) Project was launched in April 2023, spearheaded by three Japanese institutes: the Tokai National Higher Education and Research System, Institutes of Natural Sciences, Soka University. This first time that a field life sciences adopted Ministry Education, Culture, Sports, Science Technology (MEXT) for Large-scale Academic Frontiers Promotion Project. project aims to construct knowledgebase human glycans glycoproteins as standard glycome. A high-throughput...
Journal Article Affinity Capturing and Gene Assignment of Soluble Glycoproteins Produced by the Nematode Caenorhabditis elegans Get access Jun Hirabayashi, Hirabayashi 2 *Department Biological Chemistry, Faculty Pharmaceutical Sciences, Teikyo UniversitySagamiko, Kanagawa 199-0195 2To whom correspondence should be addressed. Tel: +81-426-85-3741, Fax: +81-426-85-3742, E-mail: j-hira@pharm.teikyo-u.ac.jp Search for other works this author on: Oxford Academic PubMed Google Scholar Ko Hayama,...