A5089609086- Reproductive Biology and Fertility
- Systemic Sclerosis and Related Diseases
- Ovarian function and disorders
- Extracellular vesicles in disease
- Plant Micronutrient Interactions and Effects
- Effects and risks of endocrine disrupting chemicals
- Sperm and Testicular Function
- Plant Stress Responses and Tolerance
- Trace Elements in Health
- Animal Genetics and Reproduction
- Tissue Engineering and Regenerative Medicine
- Endometriosis Research and Treatment
- Plant Pathogenic Bacteria Studies
- Birth, Development, and Health
- Environmental Toxicology and Ecotoxicology
- Reproductive System and Pregnancy
- MicroRNA in disease regulation
- Pharmaceutical and Antibiotic Environmental Impacts
- Plant-Microbe Interactions and Immunity
- Estrogen and related hormone effects
- Reproductive Physiology in Livestock
- Heavy metals in environment
- Genomics and Phylogenetic Studies
- Nitrogen and Sulfur Effects on Brassica
- Genetic and phenotypic traits in livestock
Hunan University of Humanities, Science and Technology
2014-2024
Biotechnology Institute
2023
Northwest A&F University
2006-2012
Yulin University
2006-2011
Polycystic ovary syndrome (PCOS) can cause reproductive disorders that may affect oocyte quality from punctured follicles in human follicular fluid (HFF). The non-coding RNA family includes micro (miRNA), piwi-interacting (piRNA) and transfer (tRNA); these transcripts play diverse functions are implicated a variety of diseases health conditions, including infertility. In this study, to explore the role HFF exosomes PCOS, we extracted sequenced PCOS patients compared analysis results with...
Porcine induced pluripotent stem (piPS) cell lines have been generated recently by using a cocktail of defined transcription factors, however, the features authentic piPS cells not agreed upon and most published iPS clones did meet stringent requirements pluripotency. Here, we report generation from fibroblasts retrovirus carrying four mouse factors (mOct4, mSox2, mKlf4 mc-Myc, 4F). Multiple LIF-dependent were these showed morphology similar to embryonic other cells. In addition routine...
Summary The objective of this study was to determine if insulin–transferrin–selenium (ITS) promoted a nuclear and cytoplasmic maturation porcine oocytes that better supports subsequent embryonic development. rate oocyte in vitro (IVM) an experimental group treated with hormones for 42 h significantly increased compared control without hormone treatment (47.8% vs. 11.7%, respectively, p < 0.05). Following reduction the period from 21 h, which included both first (45.4%) second (44.8%), IVM...
Abstracts Background Follicular development is crucial to normal oocyte maturation, with follicular size closely related maturation. To better understand the molecular mechanisms behind porcine we obtained exosomal miRNA from fluid (PFF). These samples were then sequenced and analyzed regarding their different sizes, as described in methods section. Results First, these results showed that this process successfully isolated PFF exosomes. Nearly all valid reads sequencing data mapped genome...
Members of the Metal Tolerance Protein (MTP) family are critical in mediating transport and tolerance divalent metal cations. Despite their significance, understanding MTP genes mustard (Brassica juncea) remains limited, especially regarding response to heavy (HM) stress. In our study, we identified gene sets Brassica rapa (17 genes), nigra (18 B. juncea (33 genes) using HMMER (Cation_efflux; PF01545) BLAST analysis. For 33 BjMTPs, a comprehensive bioinformatics analysis covering...
Previous studies have reported that the first polar body (PB1) morphology reflects embryo development competence, but effects of PB1 on porcine remain unknown. This study aims to determine whether ability is related oocytes' in vitro. The distribution type II cortical granules (CGs) matured oocytes grade B significantly greater compared with those grades A and C (71.43% versus 52.46% 50%; P 0.05). acetylation level embryos group other groups (P < 0.05), almost 2.5 times higher than A....
The Metal Tolerance Protein (MTP) family members play a crucial role in the transport and tolerance of divalent metal cations. However, comprehensive study MTP genes Brassica juncea their expression patterns under heavy (HM) exposure remains unclear. In this study, we identified 33 BjMTP through HMMER program (Cation_efflux; PF01545) BLAST analysis. Subsequently, these were subjected to series bioinformatics methods, including physicochemical properties, phylogenetic tree, conserved motifs,...
<title>Abstract</title> Members of the Metal Tolerance Protein (MTP) family are critical in mediating transport and tolerance divalent metal cations. Despite their significance, little is known about MTP genes mustard (<italic>Brassica juncea</italic>), particularly relation to how they react HM stress. In our study, we identified <italic>MTP </italic>gene sets <italic>Brassica rapa</italic> (17 genes), nigra</italic> (18 <italic>B. juncea</italic> (33 genes) using HMMER tool (Cation_efflux;...
Prochloraz is a widely used fungicide worldwide. It classified as an endocrine disrupting pesticide that affects the reproductive system. This study aimed to examine impact of exposure prochloraz male mice on system their offspring mice. Male father were intragastrically administered different dosages (group MA: 0 mg/kg/day; MB: 53.33 MD:160 mg/kg/day). Then, testicular average weight in dose groups was found be significantly lower than those control group (MB:0.312g, MD:0.294g, and MA:0.355 g;
Abstract Background: Follicular development is crucial to normal oocyte maturation, with follicular size closely related maturation. To better understand the molecular mechanisms behind porcine we obtained exosomal miRNA from fluid (PFF). These samples were then sequenced and analyzed regarding their different sizes, as described in methods section. Results： First, these results showed that this process successfully isolated PFF exosomes. Nearly all valid reads sequencing data mapped genome...
Prochloraz is a widely used fungicide in the world, which belongs to environmental endocrine disrupting estrogen pesticide of reproductive system. The purpose present study was investigate on system male offspring mice after perinatal exposure by prochloraz. different doses (group MA, MB and MD) were given father intragastric administration. results firstly showed that average testicular weight dosage groups significantly lower than control group (0.312g ,0.294g, Vs 0.355g; P < 0.05),...
Abstract The effects of hormone additions at various stages and different basic media, with or without serum, on porcine oocyte maturation in vitro were studied. results showed that the rate was not significantly three supplement; modified TCM199 medium (54.01%) higher than (46.16%) (47.14%), but these differences significant; serum-free (67.10%) plus serum (52.22%). Therefore, modifed tissue culture 199 (mTCM199)+10I U/ml pregnant mare gonadotrophin (PMSG)+10I human chorionic (hCG)+2.5...
Abstract Follicular development is crucial to oocyte maturation in these special surrounds, and the follicular size closely related maturation. In order clarify porcine molecular mechanism, obtained exosomes miRNAs from fluid (PFF) are sequenced researched among different as described methods. The results firstly show that PFF can be successfully isolated, almost all valid reads of sequencing data mapped genome database. Using hierarchical clustering methods, it secondly finds significantly...
Abstract Follicular development is crucial to normal oocyte maturation, with follicular size closely related maturation. To better understand the molecular mechanisms behind porcine we obtained exosomal miRNA from fluid (PFF). These samples were then sequenced and analyzed regarding their different sizes, as described in methods section. First, these results showed that this process successfully isolated PFF exosomes. Nearly all valid reads sequencing data mapped genome database. Second,...
Abstract Background: Follicular development is crucial to normal oocyte maturation, with follicular size closely related maturation. To better understand the molecular mechanisms behind porcine we obtained exosomal miRNA from fluid (PFF). These samples were then sequenced and analyzed regarding their different sizes, as described in methods section. Results： First, these results showed that this process successfully isolated PFF exosomes. Nearly all valid reads sequencing data mapped genome...
Abstract Follicular development is crucial to normal oocyte maturation, with follicular size closely related maturation. To better understand the molecular mechanisms behind porcine we obtained exosomal miRNA from fluid (PFF). These samples were then sequenced and analyzed regarding their different sizes, as described in methods section. First, these results showed that this process successfully isolated PFF exosomes. Nearly all valid reads sequencing data mapped genome database. Second,...