A5002466604- Renal Transplantation Outcomes and Treatments
- Renal and Vascular Pathologies
- MRI in cancer diagnosis
- Cardiac Imaging and Diagnostics
- Advanced Proteomics Techniques and Applications
- Renal Diseases and Glomerulopathies
- Organ Transplantation Techniques and Outcomes
- Neurological Complications and Syndromes
- Pregnancy and Medication Impact
- Chronic Kidney Disease and Diabetes
- Mass Spectrometry Techniques and Applications
- Primary Care and Health Outcomes
- Transplantation: Methods and Outcomes
- Organ Donation and Transplantation
- Liver Disease Diagnosis and Treatment
- Acute Lymphoblastic Leukemia research
- Acute Myeloid Leukemia Research
- COVID-19 Impact on Reproduction
- Metabolomics and Mass Spectrometry Studies
- Infectious Aortic and Vascular Conditions
- Tissue Engineering and Regenerative Medicine
- Urinary Tract Infections Management
- Biotin and Related Studies
- Scientific Computing and Data Management
- Advanced Biosensing Techniques and Applications
St James's University Hospital
2007-2022
Leeds Teaching Hospitals NHS Trust
2018-2020
University of Leeds
2018
Development Fund
2014
Fundación Juan March
2014
Cancer Research UK
2011-2013
Queen Elizabeth Hospital Birmingham
2011
General Department of Preventive Medicine
2007
Immunodepletion of clinical fluids to overcome the dominance by a few very abundant proteins has been explored but studies are few, commonly examining only limited aspects with one analytical platform. We have systematically compared immunodepletion 6, 14, or 20 using serum from renal transplant patients, analysing reproducibility, depth coverage, efficiency, and specificity 2-D DIGE ('top-down') LC-MS/MS ('bottom-up'). A progressive increase in protein number (≥2 unique peptides) was found...
We analysed data from 80 patients who tested positive for SARS-CoV-2 RNA had previously been HLA typed to support transplantation. Data were combined two adjacent centres in Manchester and Leeds achieve a sufficient number early analysis. frequencies observed compared against control populations: first, published UK deceased donor population (n = 10,000) representing the target of virus, second, using cohort individuals transplant waiting lists both 308), comparator group unaffected same...
Early identification and prognostic stratification of delayed graft function following renal transplantation has significant potential to improve outcome. Mass spectrometry analysis serum samples, before on day 2 post transplant from five patients with an uncomplicated transplant, identified aminoacylase-1 (ACY-1) as a outcome biomarker. Following assay development, longitudinal samples initial validation cohort 55 confirmed that the ACY-1 level 1 or was moderate predictor function, similar...
Late-onset proteinuria after renal transplantation has been universally associated with poor allograft outcomes. However, the significance of early low-grade posttransplant remains uncertain.We analyzed effect 3 months posttransplantation on death-censored graft loss, death a functioning graft, vascular events within graft's life, and estimated glomerular filtration rate at 5 years. Four hundred seventy-seven transplants from single center (1988-2003) mean follow-up 122 were divided into...
Diabetic kidney disease (DKD) remains one of the leading causes premature death in diabetes. DKD is classified on albuminuria and reduced function (estimated glomerular filtration rate (eGFR)) but these have modest value for predicting future renal status. There an unmet need biomarkers that can be used clinical settings which also improve prediction decline top routinely available data, particularly early stages. The iBEAt study BEAt-DKD project aims to determine whether imaging (magnetic...
Background Neutrophil gelatinase-associated lipocalin (NGAL) is a promising biomarker for acute kidney injury that beginning to be used in clinical practice addition research studies. The current study describes an independent validation and comparison of five commercially available NGAL assays, focusing on urine samples. This essential step the translation this marker use terms allowing valid inter-study generation robust results. Methods Two CE (Conformité Européenne)-marked Test...
The use of alemtuzumab as induction immunosuppression for renal transplantation introduces the possibility long-term tacrolimus monotherapy, avoiding maintenance with both corticosteroids and mycophenolate mofetil (MMF).We conducted a single-center, prospective, open-label, randomized controlled trial comparing two steroid avoidance regimens between December 2006 November 2010. One hundred sixteen adult patients were to either basiliximab followed by MMF or monotherapy. primary endpoint was...
Vascular disease and chronic allograft nephropathy have prompted re-evaluation of steroids calcineurin inhibitors (CNIs) in renal transplantation. Sirolimus (SRL) can facilitate early CNI withdrawal. We report on the Early Steroid Elimination Leeds (ECSEL) study, which was terminated due to poor tolerability SRL. Basiliximab/methylprednisolone induction used, then 2 months tacrolimus (TAC) mycophenolate mofetil (MMF) treatment. A total 51 patients were randomized continue TAC/MMF or switch...
Kidney transplants from young pediatric donors are uncommonly performed in the UK. Published literature of kidney transplant weighing less than 5 kg is sparse. We present our initial experience en bloc transplantation (EKT) 20 kg, including neonatal donors. All recipients undergoing EKT under at center January 2005 to October 2016 were included. Donor and recipient details recorded a prospective database. Electronic patient records examined for follow-up data. Of 30 EKTs included, 15 ≤5 >5...
We have examined the impact of sample processing time delay, temperature, and addition protease inhibitors (PIs) on urinary proteome peptidome, an important aspect biomarker studies.Ten urine samples from patients with varying pathologies were each divided PIs added to one-half, aliquots then processed frozen immediately, or after a delay 6 h at 4°C room temperature (20-22°C), effectively yielding 60 in total. Samples analyzed by 2D-PAGE, SELDI-TOF-MS, immunoassay.Interindividual variability...
Background Protein biomarkers with associations the activity and outcomes of diseases are being identified by modern proteomic technologies. They may be simple, accessible, cheap safe tests that can inform diagnosis, prognosis, treatment selection, monitoring disease therapy substitute for complex, invasive expensive tests. However, their potential is not yet realised. Design methods The study consisted three workstreams to create a framework research: workstream 1, methodology – define...
Abstract Use of depopulated bovine ureteric xenografts for hemodialysis vascular access has recently been described. Cellular components have removed, giving a connective tissue matrix which can be neocellularized, retaining native biomechanics. A 24‐year‐old male with end‐stage renal disease from focal segmental glomerulosclerosis presented particularly difficult access. xenograft was implanted the left subclavian artery to innominate vein. It became massively aneurysmal, requiring...
This article was originally published in Proteomics 2011, 11 , 2222–2235, DOI 10.1002/pmic.201100005
<h3>Introduction</h3> Combined liver kidney transplantation (CLKT) is an accepted approach to management of patients with dual organ pathology, but may be associated significant additional post-operative morbidity and mortality in comparison either alone. <h3>Aim</h3> To analyse the experience CLKT at a single centre. <h3>Method</h3> Retrospective analysis all CLKTs performed our centre between May 1994 August 2010. Data collected included demographics, indications for CLKT, surgical...