A5063858810- Bacterial Genetics and Biotechnology
- Salmonella and Campylobacter epidemiology
- Insect Resistance and Genetics
- Vibrio bacteria research studies
- Viral gastroenteritis research and epidemiology
- Entomopathogenic Microorganisms in Pest Control
- Clostridium difficile and Clostridium perfringens research
- CRISPR and Genetic Engineering
- Escherichia coli research studies
- Insect and Pesticide Research
- Bacteriophages and microbial interactions
- RNA Research and Splicing
- Bacillus and Francisella bacterial research
- interferon and immune responses
- Nuclear Structure and Function
- Gut microbiota and health
- Amino Acid Enzymes and Metabolism
- Polyamine Metabolism and Applications
- Biochemical and Molecular Research
- RNA Interference and Gene Delivery
- Insect symbiosis and bacterial influences
Shandong University
2010-2024
State Key Laboratory of Microbial Technology
2022-2023
Yale University
2019-2020
Tsinghua University
2010
Microbially derived, protein-based biopesticides offer a more sustainable pest management alternative to synthetic pesticides. Vegetative insecticidal proteins (Vip3), multidomain secreted by Bacillus thuringiensis, represent second-generation toxin that has been preliminarily used in transgenic crops. However, the molecular mechanism underlying Vip3's toxicity is poorly understood. Here, we determine distinct functions and contributions of domains Vip3Aa protein its against Spodoptera...
Typhoid toxin is a virulence factor for Salmonella Typhi and Paratyphi, the cause of typhoid fever in humans. This has unique architecture that its pentameric B subunit, made PltB, linked to two enzymatic A subunits, ADP ribosyl transferase PltA deoxyribonuclease CdtB. uniquely adapted humans, recognizing surface glycoprotein sialoglycans terminated acetyl neuraminic acid, which are preferentially expressed by human cells. The transport pathway cellular targets followed after receptor...
Salmonella Typhi is a human host-restricted pathogen that responsible for typhoid fever in approximately 10.9 million people annually1. The toxin postulated to have central role disease pathogenesis, the establishment of chronic infection and host restriction2–6. However, its precise humans not fully defined. We studied acute using randomized, double-blind S. challenge model7. Forty healthy volunteers were randomized (1:1) oral with 104 colony-forming units wild-type or an isogenic deletion...
Abstract Bacterial toxins with an AB 5 architecture consist of active (A) subunit inserted into a ring-like platform comprised five delivery (B) subunits. Salmonella Typhi, the cause typhoid fever, produces unusual A 2 B toxin known as toxin. Here, we report that upon infection human cells, S . Typhi two forms have distinct components but share common The exhibit different trafficking properties, elicit effects when administered to laboratory animals, and are expressed using regulatory...
Efficient interbacterial competitions and diverse defensive strategies employed by various bacteria play a crucial role in acquiring hold within dense microbial community. The gut symbiont Bacteroides fragilis secretes an antimicrobial ubiquitin homologue (BfUbb) that targets essential periplasmic PPIase to drive intraspecies bacterial competition. However, the mechanisms which BfUbb enters periplasm its potential for interspecies antagonism remain poorly understood. Here, we employ...
Microbially derived, protein-based biopesticides have become a vital element in pest management strategies. Vip3 family proteins from
Typhoid toxin is an A2B5 protein and important virulence factor for the human-adapted bacterial pathogen Salmonella enterica serovar Typhi, causative agent of typhoid fever. contains two enzymatic subunits, PltA CdtB, which dock onto a pentameric delivery platform composed PltB. It was recently reported that same subunits can assemble with different PltC, forming distinct version toxin. However, differences in structure receptor specificity between PltC PltB toxins remain unknown. Here, we...
Typhoid toxin is a virulence factor of Salmonella enterica serovar Typhi, the causative agent typhoid fever, and thought to be responsible for symptoms severe disease. This has unique A2B5 architecture with two active subunits, ADP ribosyl transferase PltA DNase CdtB, linked pentameric B subunit, which alternatively made PltB or PltC. Here, we describe generation characterization toxin-neutralizing human monoclonal antibodies by immunizing genetically engineered mice that have full set...
Microbially derived, protein-based biopesticides are an important approach for sustainable pest management. The secreted insecticidal proteins (Sips) produced by the bacterium Bacillus thuringiensis exhibit potent activity against coleopteran pests and are, therefore, attractive as candidate biopesticides. However, modes-of-action of Sips unclear comprehensive structural information these is lacking.Using X-ray crystallography, we elucidated structure monomeric Sip1Ab at 2.28 Å resolution....
Abstract Bacterial toxins with an AB 5 architecture are central to bacterial pathogenesis. Functionally diverse and evolutionarily distant adopt synonymous structures in which a discrete domain of the toxin’s active (A) subunit is inserted into ring-like platform comprised five delivery (B) subunits. Salmonella Typhi, cause typhoid fever, produces unusual A 2 B toxin known as toxin, major virulence factor. Here, we report that upon infection human cells, S . Typhi two forms have distinct...
Abstract Homozygous mutations in Ig-like domain of LMNA cause severe progeria. Unlike typical HGPS mediated by progerin due to LMNAWT/G608G mutation, it remains elusive how these homozygous We here found that patients with LMNAR527C/R527C mutation developed an atypical progeria autoimmune symptoms. Compare this led more inflammation patients. MSCs from exhibited overt and cellular senescence. Mechanistically, attenuated its binding DNA protein BAF, which aberrant aggregation Lamin A...
Abstract Microbially derived, protein-based biopesticides offer a more sustainable pest management alternative to synthetic pesticides. V egetative insecticidal p roteins (Vip3), multidomain proteins secreted by Bacillus thuringiensis, represent second-generation toxin that have been used in transgenic crops. However, the molecular mechanism underlying Vip3’s toxicity is poorly understood. Here, we determine distinct functions and contributions of domains Vip3Aa protein its against...