Problem: Only a small proportion of HPV+ women develop virus‐associated lesions and cervical cancer, suggesting that other factors are involved in keratinocyte transformation. Immune response plays an important role clearing HPV infection, host genetic variants resulting defective immune have been associated with virus persistence and/or cancer. Considering variations inflammasome genes were previously viral infection cancer development, the present study investigates selected single...

Abstract The most commonly used Parkinson's disease (PD) treatment is the replacement of dopamine by its levodopa precursor ( l ‐dopa). Monoamine oxidase‐B (MAO‐B) and catechol‐o‐methyl transferase (COMT) are enzymes involved in metabolism regulation availability. In our study we investigated possible relation among selected single‐nucleotide polymorphisms (SNPs) MAO‐B (rs1799836) COMT (rs4680) genes therapeutic response to A total 162 Brazilian patients from Pro‐Parkinson service Clinics...

Abstract: Patients with an autoimmune condition are known to be at higher risk of developing other disorders. Type 1 diabetes may associated additional disorders including thyroid disease. The aim this study was investigate the prevalence autoantibodies in a group children, adolescents, and young adults type from northeastern Brazil as well their significance for development design cross-sectional descriptive, analyzing people previous diagnosis. Two hundred fourteen children adolescents...

Objective: To undertake a literature review on the development of cervical cancer, emphasizing epidemiological data, human papillomavirus (HPV) infection in carcinogenesis, and role XRCC1 gene DNA repair, order to provide valuable information for medical scientific community. The goal is resource clinical practice. Bibliographic Review: Cervical cancer ranks as fourth most common malignancy worldwide. Despite known environmental, behavioral, genetic risk factors, their management remains...

Abstract Objectives The aim of this study was to evaluate a possible relationship between DRD2/ANKK1 (rs1800497) and SLC6A3/DAT1 (rs28363170) gene polymorphisms with the response levodopa (L-DOPA)-therapy in patients Parkinson's disease (PD). Methods One hundred ninety-five idiopathic PD were investigated. Patients genotyped for rs1800497 rs28363170 using PCR-RFLP. Logistic regression performed assess association occurrence chronic complications L-DOPA therapy. Key findings Our results...

The Human Papillomavirus (HPV) predisposes 500 000 women to cervical cancer. Host genetic background may facilitate virus persistence in the uterine cervix. Polymorphisms regulatory and coding regions of cytokine genes have been associated with susceptibility some human diseases.This study aims at investigating whether TNFA -308 G/A IL18 -137 G/C -607 C/A polymorphisms are HPV infection/progression high-grade squamous intraepithelial lesion (HSIL).One hundred twenty-two infected 132 negative...

Abstract Objectives: to determine the incidence of main high oncogenic risk types human papillomavirus (HPV) ( 16, 18, 31 and 33) factors for cervical adenocarcinoma. Methods: a case-control study was carried out with 324 women (69 adenocarcinoma 260 healthy controls) between 2001 2014. Information on associated adenocarcinomawere collected detection performed HPVby using Polymerase Chain Reaction (PCR) method. Results: age ≥40 years old (OR=2.95; 95%CI=1.13-7.71), ≤3 schooling (OR=2.34;...

The aim of the present study was to evaluate presence periodontal pathogens that form red complex (Tannerella forsythia, Porphyromonas gingivalis and Treponema denticola) Aggregatibacter actinomycetemcomitans in patients with chronic periodontitis. sample consisted 29 a clinical radiographic diagnosis periodontitis based on criteria American Academy Periodontology (3). Samples for microbiological analysis were collected from four sites greatest probing depth each patient, totaling 116...

This study aimed to evaluate the antimicrobial activity of a dentifrice containing an alcoholic extract rosemary on oral bacteria, compared commercially available herbal dentifrice. Standard strains Streptococcus mutans (ATCC 25175), oralis 9811) and Lactobacillus rhamnosus 7469) were used, as well different toothpastes based (TR), propolis (TH), triclosan (positive control) (TPC) non-fluoridated (negative (TNC). Bacteria seeded in Petri dishes paper discs soaked with dilutions placed...

Abstract Spermadhesins are the major proteins of boar seminal plasma and form a group polypeptides probably involved in reproduction. In previous work, member spermadhesin family from buck plasma, called BSFP, was characterized by mass spectrometry N‐terminal sequencing. The present study aimed to clone characterize BSFP gene investigate its expression along genital tract using real‐time polymerase chain reaction (PCR). cDNAs vesicle, testis, epididymis, bulbourethral gland, ductus deferens...

Abstract Polymorphisms in interleukin (IL) ‐18 , IL ‐12 and interferon (IFN) ‐γ genes are associated with different levels of cytokines expression have been rheumatoid arthritis ( RA ). +105 A/C, IL‐12B +1188 A/C IFN +874 T/A polymorphisms were analyzed by restriction fragment length polymorphism‐polymerase chain reaction (PCR) amplification refractory mutation system PCR from 90 patients 186 healthy individuals. There significant differences to polymorphism between the control groups (odds...

In the present study, we compared performance of a ThinPrep cytological method with conventional Papanicolaou test for diagnosis cytopathological changes, regard to unsatisfactory results achieved at Central Public Health Laboratory State Pernambuco. A population-based, cross-sectional study was performed women aged 18 65 years, who spontaneously sought gynecological services in Units Pernambuco, Northeast Brazil, between April and November 2011. All patients were given standardized...

Rheumatoid arthritis (RA) is an autoimmune disease which can lead to progressive and functional disability. Literature data suggest that some inflammatory proteins are dysregulated in RA patients its genetic polymorphisms may contribute the aetiology pathogenesis of different ethnic groups. Polymorphisms IL1β, IL18, NFKB1 IFNG genes were studied populations with RA, but analysis indicated contradictory results. Thereby, we hypothesised these could have a combined effect on susceptibility...

We report a fast (less than 3 h) and cost-effective melting temperature assay method for the detection of single-nucleotide polymorphisms in MBL2 gene. The protocol, which is based on Corbett Rotor Gene real time PCR platform SYBR Green I chemistry, yielded, cohorts studied, sensitive (100%) specific amplification without use costly fluorophore-labeled probes or post-PCR manipulation. At end PCR, dissociation protocol included slow heating from 60º to 95ºC 0.2ºC steps, with an 8-s interval...

Human papillomavirus (HPV) infections are strongly associated with the development of cervical intraepithelial neoplasias and invasive cancer. Polymorphisms in cytokine-encoding genes behavioural cofactors could play an important role protecting individual against viral Here, we investigated whether IL-6 -174 G>C, IL-8 +396 G>T, TGF-β1 +869 G>C +915 polymorphisms were susceptibility to HPV infection women from north-east (Pernambuco) Brazil. We analysed 108 healthy uninfected (HC)...

Enterococcus faecium is a lactic acid bacterium with applications in food engineering and nutrigenomics, including as starter cultures fermented foods. To differentiate the E. probiotic from pathogenic bacteria, physiological analyses are often used but they do not guarantee that bacterial strain pathogenic. We report here new findings an approach based on comparison of genetic mobility (1) probiotic, (2) pathogenic, (3) nonpathogenic non-probiotic strains, so to probiotics, inform their...

Polymorphisms in chemokine receptors play an important role the progression of cervical intraepithelial neoplasia (CIN) to cancer (CC). Our study examined association CCR2-64I (rs1799864) andCCR5-Δ32 (rs333) polymorphisms with susceptibility develop lesion (CIN and CC) a Brazilian population. The genotyping 139 women lesions 151 without for CCR5-Δ32 were performed using polymerase chain reaction-restriction fragment length polymorphism. individuals carrying heterozygous or homozygous...

Background . Genetic polymorphisms in certain cytokines and chemokines have been investigated to understand why some individuals display implant flaws despite having few risk factors at the time of implant. Purpose To investigate association genetic interleukin- (IL-) 10 [-1082 region (A/G)] RANKL [-438 with failure dental implants. Materials Methods This study included 90 partially edentulous male female patients who were rehabilitated a total 245 Straumann An was considered if any...