A5101844984- Reproductive tract infections research
- Cervical Cancer and HPV Research
- Bacterial Infections and Vaccines
- Syphilis Diagnosis and Treatment
- Urinary Tract Infections Management
- Parasitic Infections and Diagnostics
- Virology and Viral Diseases
- Reproductive System and Pregnancy
- Biochemical and Structural Characterization
- Influenza Virus Research Studies
- Helminth infection and control
- Amoebic Infections and Treatments
- Respiratory viral infections research
- Animal Disease Management and Epidemiology
- Bacterial Identification and Susceptibility Testing
- Coccidia and coccidiosis research
Ambedkar University Delhi
2009-2020
University of Delhi
2009-2020
University of Nottingham
2017
Abstract Background Screening women for Chlamydia trachomatis infection in developing countries is highly desirable because of asymptomatic infection. The existing diagnostic methods are not effective and their sensitivity fall below 45.0% which leads to further spread There an urgent need improved cost tests that will reduce the burden sexually transmitted infections world. Methods Prevalence C. among visiting gynaecology department Hindu Rao hospital Delhi, India was determined using Roche...
To improve the control of Chlamydia trachomatis infection in India, a rapid, specific and cost-effective method is much needed. We developed an in-house PCR assay by targeting unique genomic sequence encoding protein from C. phospholipase D endonuclease superfamily that produces amplified fragment 368 bp. The specificity primers was confirmed using DNA other sexually transmitted disease-causing related micro-organisms humans. highly sensitive could detect as low 10 fg DNA. Clinical...
Fasciola hepatica infection of ruminants leads to non-resolving chronic infection, as patency develops there is switching a TGF-β and IL-10 led response. Here we explore the responses CD4 T-cells within major draining lymph nodes. We found minimal expression Foxp3 cells but elevated levels γδ (WC1+) population. There strong T-cell intrinsic exhaustion phenotype hepatic node (HLN) characterised by lack antigen-specific proliferation cytokine secretion. recovered from HLN had high PD-1 low...
Neisseria gonorrhoeae is an important sexually transmitted diseases (STD) causing pathogen worldwide. Due to absence of affordable diagnostic assay, routine screening gonococcal infection becomes impossible in developing countries where rates are maximum. Treatment given on the basis symptoms alone which leads spread infection. Thus, development a rapid, sensitive, specific, and PCR based visual assay suitable for countries, required better disease management, aimed at present study....
Mutations in PenA, PorB, MtrE and MtrR genes responsible for antimicrobial resistance were checked 27 drug-resistant clinical isolates of Neisseria gonorrhoeae (NG). Phenotype PIB (88.88%) mutation at G120 A121 positions porB recurrent. N122K, a novel mutation, was observed PorB three resistant isolates. Substitution H105Y widespread (37% isolates). The presence (L33V), along with G45D MtrR, associated less-resistant isolates, contrast to alone. African-type penicillinase-producing NG...
The development of an accurate, sensitive, specific, rapid, reproducible, stable-at-room-temperature and cost-effective diagnostic kit, a low-cost portable fluorescence detector to fulfil the requirements facilities in developing countries.We developed 'Chlamy Ness CT/NG kit' based on molecular beacons for detection Chlamydia trachomatis (CT) Neisseriagonorrhoeae (NG). Multi-centric evaluation kit was performed using commercially available nucleic acid amplification test (NAAT)-based FTD...
Chlamydia trachomatis (CT) is an important cause of sexually transmitted genital tract infections (STIs) and trachoma. Despite major research into chlamydial pathogenesis host immune responses, immunoprotection has been hampered by the incomplete understanding protective immunity in tract. Characterized vaccine candidates have shown variable efficacy ranging from no protection to partial vivo . It therefore a priority identify novel antigens that may elicit responses against CT infection. In...
Background Despite the recent advances in diagnosis, Neisseraia gonorrhoeae (NG) and Chlamydia trachomatis (CT) remain leading cause of STDs worldwide account for STI's morbidity to acquisition HIV HPV infection. Coinfection gonococcus is reported from various countries. In study we high rate infection by CT using an house developed PCR method. A number studies also suggest increasing antibiotic resistance NG. Thus a rapid, specific cost effective diagnostic method detect prevalence co NG...
<h3>Background</h3> Neisseria gonorrhoeae, a major STD causing pathogens, tends to pose high burden of morbidity that is borne disproportionately by women and infants with approximately 2/3rd cases from developing countries. In the absence appropriate vaccine rapid, easy, economical test, antibiotic therapy recommended for treatment on basis clinical symptoms. This has led emergence resistant strains. Since increasing antimicrobial resistance makes as super bug, we have tried elucidate...
Abstract MtrR is a negative regulator of MtrCDE efflux pump. Various N-Terminal and C-Terminal mutations have been reported in different multidrug resistant clinical isolates Neisseria gonorrhoeae across the world. Mutations N-terminal region MtrR, are known to abrogate its binding with promoter thereby providing an evidence that this encodes for DNA domain. In contrast, mechanism action C-terminal, play role protein dimerization has site ligand binding, left unexplored. present study, using...
<h3>Introduction</h3> About 300 million new infections of gonorrhoea, Chlamydia, or trichomoniasis occur each year. Young adults, population with health inequities and people in resource poor settings bear the significant proportion sexually transmitted (STI). Therefore, rapid, inexpensive acceptable tests are needed to address STIs developing countries. PCR based recommended for diagnosis several STIs, but such require sophisticated infrastructure, need be imported hence cannot used limited...
Background Chlamydia trachomatis (CT) is an obligate intracellular parasite which causes STD and trachoma. Despite major research into Chlamydial pathogenesis host immune responses, immuno-protection has been hampered by the incomplete understanding of protective immunity in genital tract. Characterised vaccine candidates general have shown variable efficacy ranging from no protection to partial protection. It therefore a priority identify novel antigens that may elicit responses. Objectives...
Background In India, knowledge regarding N. gonorrhoeae antimicrobial resistance profiling is limited, and data concerning genetic characteristics of also lacking. Herein, we investigated the determinants for various antimicrobials used against isolated in Delhi, India. Various studies have shown that this towards could be either plasmid or chromosomal mediated involving mutations genes. Methods Molecular basis was analysed by amplifying sequencing most target genes, pen A por B,...
Background Due to absence of cost effective and rapid diagnostic test syndromic management Neisseria Chlamydia was recommended in developing countries. Being nonspecific such a strategy has resulted over treatment leading increased drug resistance. It also misses out on asymptomatic patients resulting increase disease burden. The study describes the development evaluation low duplex PCR method (dPCR) for co-detection Chlamydia. Using molecular beacons we further provide quantitative easy...