A5075755687- Genetic factors in colorectal cancer
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Cytokine Signaling Pathways and Interactions
- Immune Response and Inflammation
- Bioinformatics and Genomic Networks
- Cancer Genomics and Diagnostics
- Gene expression and cancer classification
- NF-κB Signaling Pathways
- IL-33, ST2, and ILC Pathways
- Biomedical Text Mining and Ontologies
- RNA and protein synthesis mechanisms
- T-cell and B-cell Immunology
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Cancer Immunotherapy and Biomarkers
- interferon and immune responses
- Rheumatoid Arthritis Research and Therapies
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- vaccines and immunoinformatics approaches
- Genomics and Phylogenetic Studies
- Immune Cell Function and Interaction
- Influenza Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
The University of Texas MD Anderson Cancer Center
2021-2025
Union Hospital
2024
Huazhong University of Science and Technology
2020-2024
Wuhan National Laboratory for Optoelectronics
2023
First Affiliated Hospital of Gannan Medical University
2023
Sun Yat-sen University
2021-2022
Cedars-Sinai Medical Center
2021
Huazhong Agricultural University
2021
Translational Research Informatics Center (Japan)
2021
The University of Texas Health Science Center at Houston
2018-2020
Abstract The accumulation of unfolded or misfolded proteins within the endoplasmic reticulum (ER), due to genetic determinants and extrinsic environmental factors, leads stress (ER stress). As ER ensues, protein response (UPR), comprising three signaling pathways—inositol-requiring enzyme 1, kinase R-like kinase, activating transcription factor 6 promptly activates enhance ER’s protein-folding capacity restore homeostasis. However, prolonged levels propels UPR towards cellular demise...
DNA mismatch repair deficiency drives microsatellite instability (MSI). Cells with MSI accumulate numerous frameshift mutations. Frameshift mutations affecting cancer-related genes may promote tumorigenesis and, therefore, are shared among independently arising tumors. Consequently, such recurrent can give rise to immunogenic peptides (FSPs) that represent ideal candidates for a vaccine against cancer. Pathogenic germline variants of cause Lynch syndrome (LS), hereditary cancer approximately...
Background & Aims Lynch syndrome (LS) carriers develop mismatch repair deficient (MMRd) neoplasia with high neoantigen (neoAg) rates. No detailed information on targetable neoAgs from LS pre-cancers exists, which is crucial for vaccine development and immune-interception strategies. We report a focused somatic mutation frameshift-neoAg landscape of microsatellite loci colorectal polyps without malignant potential (PWOMPs), early-stage cancers in carriers. Methods generated paired whole-exome...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMicellization and gelation of block-copoly(oxyethylene/oxybutylene) in aqueous solutionSiriporn Tanodekaew, Nan Jie Deng, Sibyl Smith, Yung Wei Yang, David Attwood, Colin BoothCite this: J. Phys. Chem. 1993, 97, 45, 11847–11852Publication Date (Print):November 1, 1993Publication History Published online1 May 2002Published inissue 1 November 1993https://pubs.acs.org/doi/10.1021/j100147a044https://doi.org/10.1021/j100147a044research-articleACS...
Gene Expression Omnibus (GEO) and other publicly available data store their metadata in the format of unstructured English text, which is very difficult for automated reuse.We employed text mining techniques to analyze GEO developed Restructured database (ReGEO). ReGEO reorganizes categorizes series makes them searchable by two new attributes extracted automatically from each series' metadata. These are number time points tested experiment disease being investigated. also GEO, such as...
Colorectal pre-cancers in Lynch Syndrome (LS) exhibit a distinct immune profile, presenting unique opportunities for developing immune-interception strategies to prevent carcinogenesis. Epigenetic modulation by EZH2 of immune-related genes is implicated the carcinogenesis different cancer types including colorectal. This study utilizes mouse model LS and ex vivo colonic organoids assess effects inhibitor GSK503 on regulatory pathways, tumorigenesis, epigenetic reprogramming. Our findings...
Introduction: Familial adenomatous polyposis (FAP) is a hereditary syndrome caused by germline alterations in APC and characterized the presence of multiple colorectal adenomas that are associated to high risk cancer (CRC) development. mutations dysregulate WNT signaling leading aberrant β-catenin activity. The Farnesoid X Receptor (FXR) key regulator bile acid homeostasis has been identified as suppressor intestinal tumorigenesis. FXR downregulation shown exacerbate activity, further...
Abstract Background: Lynch syndrome (LS) is a hereditary cancer predisposition caused by germline mutations in mismatch repair (MMR) genes, with secondary somatic alterations leading to MMR deficiency (dMMR) and increased mutation rates. These often result frameshift peptides (FSPs) that act as immunogenic neoantigens (neoAgs), triggering immune responses expanding specific T cell clonotypes. NeoAg-specific cells LS carriers have significant potential for tumor surveillance. This study...
Abstract Purpose: Lynch syndrome (LS) is a hereditary condition with high lifetime risk of colorectal and endometrial cancers. Exercise non-pharmacologic intervention to reduce cancer risk, though its impact on patients LS has not been prospectively studied. Here, we evaluated the 12-month aerobic exercise cycling in biology immune system carriers. Patients Methods: To address this, enrolled 21 onto non-randomized, sequential assignation, clinical trial assess effect program that included...
Within overall Th1-like human memory T cell responses, individual cells may express only some of the characteristic Th1 cytokines when reactivated. In Th1-oriented response to influenza, we have tested contributions two potential mechanisms for this diversity: variable expression by a uniform population during activation, or different stable subsets that consistently expressed cytokine pattern. To test short-term variability, in vitro-stimulated influenza-specific CD4+ were sorted according...
Cronkhite-Canada Syndrome (CCS) is a rare, noninherited polyposis syndrome affecting 1 in every million individuals. Despite over 50 years of CCS cases, the etiopathogenesis and optimal treatment for remains unknown due to rarity disease lack model systems. To better understand etiology CCS, we generated human intestinal organoids (HIOs) from stem cells isolated 2 patients. We discovered that HIOs are highly proliferative have increased numbers enteroendocrine producing serotonin (also known...
Abstract Topologically associated domains (TADs) are one of the important higher order chromatin structures with various sizes in eukaryotic genomes. TAD boundaries, as flanking regions between adjacent domains, can restrict interactions regulatory elements, including enhancers and promoters, generally dynamic variable different cells. However, influence sequence epigenetic profile-based features identification boundaries is largely unknown. In this work, we proposed a method called pTADS...
Colorectal cancer (CRC) remains the third most common in US with 15% of cases displaying Microsatellite Instability (MSI) secondary to Lynch Syndrome (LS) or somatic hypermethylation MLH1 promoter. A cohort rhesus macaques from our institution developed spontaneous mismatch repair deficient (MMRd) CRC a notable fraction harboring pathogenic germline mutation (c.1029C<G, p.Tyr343Ter). Our study aimed provide detailed molecular characterization for cross-comparison human MMRd CRC. We...
Background Recent clinical trial data from Lynch Syndrome (LS) carriers demonstrated that naproxen administered for 6-months is a safe primary chemoprevention promotes activation of different resident immune cell types without increasing lymphoid cellularity. While intriguing, the precise enriched by remained unanswered. Here, we have utilized cutting-edge technology to elucidate activated in mucosal tissue LS patients. Methods Normal colorectal mucosa samples (pre- and post-treatment)...
// Arvand Asghari 1 , * Katherine Wall 2 Michael Gill Natascha Del Vecchio 3 Farnaz Allahbakhsh Jacky Wu Nan Deng 4 W. Jim Zheng 5 Hulin Michihisa Umetani 6 and Vahed Maroufy Center for Nuclear Receptors Cell Signaling, Department of Biology Biochemistry, University Houston, TX 77204, USA Biostatistics Data Science, School Public Health, UTHealth, 77030, Chicago HIV Elimination, Chicago, IL 60637, Clinical Cancer Prevention Department, Texas MD Anderson Center, Biomedical Informatics, Health...
The cytokine secretion assay identifies live cytokine-secreting cells by capturing the secreted on a surface-bound capture antibody in dilute suspension culture, followed detection with fluorescent anti-cytokine antibody. However, examining kinetics of revealed that IL-2 staining reached maximum at early times and then declined, whereas for other cytokines including interferon (IFNγ) increased up to 90 min. decline could have been due rapid cessation synthesis, coupled internalization...
Background Microsatellite instability (MSI) secondary to mismatch repair (MMR) deficiency is characterized by insertions and deletions (indels) in short DNA sequences across the genome. These indels can generate neoantigens, which are ideal targets for precision immune interception. However, current neoantigen databases lack information on neoantigens arising from coding microsatellites. To address this gap, we introduce The MicrOsatellite Neoantigen Discovery Tool (MONET). Method MONET...
The mouse strain BALB/c deficient in IL-1 receptor antagonist protein (Il-1ra) develops spontaneous arthritis disease (SAD) while the DBA/1 IL1rn -/- with same deficiency does not. Previously, we mapped a QTL on chromosome 1 for SAD and then developed congenic BALB.D1-1-/- that contains genomic fragment associated resistance from DBA/1-/- BALB/c-/- background. was relatively resistant to had delayed onset reduced severity of disease. We obtained whole genome expression profiles spleen four...
Introduction: Constitutive activation of NF-κB has been implicated as being contributive to cancer cell growth, drug resistance, and tumor recurrence in many cancers including breast cancer. Activation leads nuclear translocation RelA, a critical component the transcription factor complex, which subsequently binds specific DNA sites activates multitude genes involved diverse functions. Studies show that triple-negative (TNBC) cells possess constitutively active concomitantly have higher...