A5047571737- Gut microbiota and health
- Antifungal resistance and susceptibility
- Probiotics and Fermented Foods
- Fungal Infections and Studies
- Fungal and yeast genetics research
- Clostridium difficile and Clostridium perfringens research
- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- Carbohydrate Chemistry and Synthesis
- Inflammatory Bowel Disease
- Helicobacter pylori-related gastroenterology studies
- Microbial Metabolic Engineering and Bioproduction
- Microscopic Colitis
- Peptidase Inhibition and Analysis
- Microbial Metabolites in Food Biotechnology
- Cancer Research and Treatments
- Tryptophan and brain disorders
- Gastrointestinal motility and disorders
- Immune Response and Inflammation
- Gene Regulatory Network Analysis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cultural Insights and Digital Impacts
- Enhanced Recovery After Surgery
- Biofuel production and bioconversion
- Heavy Metals in Plants
Microbiologie de l’alimentation au service de la santé
2014-2024
AgroParisTech
2013-2024
Fédération Hospitalo-Universitaire, Paris Center for Microbiome Medicine
2021-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Université Paris-Saclay
2016-2024
Institut thématique Immunologie, inflammation, infectiologie et microbiologie
2015-2017
Pitié-Salpêtrière Hospital
2017
Sorbonne Université
2016-2017
Physique des interactions ioniques et moléculaires
2017
Assistance Publique – Hôpitaux de Paris
2016
The bacterial intestinal microbiota plays major roles in human physiology and IBDs. Although some data suggest a role of the fungal IBD pathogenesis, available are scarce. aim our study was to characterise faecal patients with IBD.Bacterial composition 235 38 healthy subjects (HS) determined using 16S ITS2 sequencing, respectively. obtained sequences were analysed Qiime pipeline assess diversity. Bacterial taxa associated clinical parameters identified multivariate association linear models....
Abstract Dietary lipids favor the growth of pathobiont Bilophila wadsworthia , but relevance this expansion in metabolic syndrome pathogenesis is poorly understood. Here, we showed that B. synergizes with high fat diet (HFD) to promote higher inflammation, intestinal barrier dysfunction and bile acid dysmetabolism, leading glucose dysmetabolism hepatic steatosis. Host-microbiota transcriptomics analysis reveal pathways, particularly butanoate metabolism, which may underlie effects mediated...
Background and Aims: Gut microbiota is involved in many physiological functions its imbalance associated with several diseases, particularly inflammatory bowel diseases. Mucosa-associated could have a key role induction of host immunity process. Although the fungi has been suggested disease pathogenesis, fungal not yet deeply explored. Here we analysed bacterial composition mucosa-associated Crohn's patients healthy subjects.
Gut interleukin-17A (IL-17)-producing γδ T cells are tissue-resident that involved in both host defense and regulation of intestinal inflammation. However, factors regulate their functions poorly understood. In this study, we find the gut microbiota represses IL-17 production by cecal cells. Treatment with vancomycin, a Gram-positive bacterium-targeting antibiotic, leads to decreased short-chain fatty acids (SCFAs) microbiota. Our data reveal these microbiota-derived metabolites,...
Gut microbiota dysbiosis has been associated with inflammatory bowel diseases (IBD). In colorectal cancer, the gut also recognized as potentially involved in aggravating or favoring tumor development. However, very little is known on structure and role of colitis cancer (CAC), an important complication IBD human. Here we analyzed bacterial fungal composition mucosa patients suffering CAC, sporadic (SC) healthy subjects (HS) by barcode sequences analysis following cohort: 7 CAC patients, 10...
The extent to which tryptophan (Trp) metabolism alterations explain or influence the outcome of inflammatory bowel diseases (IBDs) is still unclear. However, several Trp end-products are essential intestinal homeostasis. Here, we investigated role metabolites from kynurenine pathway.Targeted quantitative metabolomics was performed in two large human IBD cohorts (1069 patients with IBD). Dextran sodium sulphate-induced colitis experiments mice were used evaluate effects identified...
The outer layer of the Candida albicans cell wall is enriched in highly glycosylated proteins. major class, GlycosylPhosphatidylInositol (GPI)-anchored proteins are tethered to by GPI-anchor remnants and include adhesins, glycosyltransferases, yapsins superoxide dismutases. In silico analysis suggested that C. possesses 115 putative GPI anchored (GpiPs), almost twice number reported for Saccharomyces cerevisiae. A global approach characterise predicted GpiPs has been initiated generating a...
Host-microbe balance maintains intestinal homeostasis and strongly influences inflammatory conditions such as bowel diseases (IBD). Here we focused on bacteria-fungi interactions their implications inflammation, a poorly understood area. Dextran sodium sulfate (DSS)-induced colitis was assessed in mice treated with vancomycin (targeting gram-positive bacteria) or colistin Enterobacteriaceae) supplemented either Saccharomyces boulardii CNCM I-745 Candida albicans. Inflammation severity well...
ABSTRACT Biofilm formation plays a key role in the life cycles and subsistence of many microorganisms. For human fungal pathogen Candida albicans , biofilm development is arguably virulence trait, because medical implants that serve as substrates are significant risk factors for infection. The C. biofilms vitro proceeds through an early phase, which yeast cells populate substrate, intermediate pseudohyphal hyphal cell types produced, maturation continued growth accompanied by accumulation...
Clostridium difficile infection (CDI) is a common complication in inflammatory bowel disease (IBD) and has been associated with poor IBD outcome. Intestinal microbiota composition patients CDI not specifically evaluated to date. The fecal of 56 patients, including 8 flare concomitant CDI, 24 without remission, as well healthy subjects, was studied using 16S sequencing. Analysis performed the Qiime pipeline. Compared had more pronounced dysbiosis higher levels Ruminococcus gnavus Enterococcus...
Inflammatory bowel disease (IBD) results from a combination of genetic predisposition, dysbiosis the gut microbiota and environmental factors, leading to alterations in gastrointestinal immune response chronic inflammation. Caspase recruitment domain 9 (Card9), one IBD susceptibility genes, has been shown protect against intestinal inflammation fungal infection. However, cell types mechanisms involved CARD9 protective role remain unknown.We used dextran sulfate sodium (DSS)-induced adoptive...
The gut microbiota is now recognized as a key parameter affecting the host’s anti-cancer immunosurveillance and ability to respond immunotherapy. Therefore, optimal modulation for preventive therapeutic purposes very appealing. Diet one of most potent modulators microbiota, thus nutritional intervention could be exploited improve host immunity. Here, we show that an inulin-enriched diet, prebiotic known promote immunostimulatory bacteria, triggers enhanced Th1-polarized CD4 + CD8 αβ T...
Introduction S taphylococcus epidermidis is a commensal bacterium ubiquitously present on human skin. This species considered as key member of the healthy skin microbiota, involved in defense against pathogens, modulating immune system, and wound repair. Simultaneously, S. second cause nosocomial infections an overgrowth has been described disorders such atopic dermatitis. Diverse isolates co-exist Elucidating genetic phenotypic specificities these health disease to better understand their...
Abstract Background Effects of antibiotics on gut bacteria have been widely studied, but very little is known about the consequences such treatments fungal microbiota (mycobiota). It commonly believed that load increases in gastrointestinal tract following antibiotic treatment, better characterization clearly needed how directly or indirectly affect mycobiota and thus entire microbiota. Design We used samples from humans (infant cohort) mice (conventional human microbiota-associated mice) to...
Biofilm formation is an important virulence trait of the pathogenic yeast Candida albicans. We have combined gene overexpression, strain barcoding and microarray profiling to screen a library 531 C. albicans conditional overexpression strains (∼10% genome) for genes affecting biofilm development in mixed-population experiments. The 16 increased occupancy within multi-strain biofilm, whereas 4 decreased it. set was significantly enriched those encoding predicted glycosylphosphatidylinositol...
Abstract Background Innate immunity genes have been reported to affect susceptibility inflammatory bowel diseases (IBDs) and colitis in mice. Dectin-1, a receptor for fungal cell wall β-glucans, has clearly implicated gut microbiota modulation modification of the inflammation. Here, we explored role Dectin-1 Dectin-2 (another molecules) deficiency intestinal Design Susceptibility dextran sodium sulfate (DSS)-induced was assessed wild-type, knockout (KO), Dectin-2KO, double Dectin-1KO...
The chlamydospore is a distinctive morphological feature of the fungal pathogen Candida albicans that can be induced to form in oxygen-limited environments and has been reported clinical specimens. Chlamydospores are not produced by model yeasts Saccharomyces cerevisiae Schizosaccharomyces pombe , so there limited understanding pathways govern their development. Here, results forward genetic approach begins define control formation described. Six genes – ISW2 MDS3 RIM13 RIM101 SCH9 SUV3...