A5005168737- Ocular Surface and Contact Lens
- Cardiac electrophysiology and arrhythmias
- Salivary Gland Disorders and Functions
- Ion channel regulation and function
- Cell Adhesion Molecules Research
- Neuroscience and Neuropharmacology Research
- Glaucoma and retinal disorders
- Cellular Mechanics and Interactions
- Malaria Research and Control
- Advanced Glycation End Products research
- Cardiovascular Effects of Exercise
- Redox biology and oxidative stress
- Immune Response and Inflammation
- Reproductive Biology and Fertility
- Mass Spectrometry Techniques and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Lipid Membrane Structure and Behavior
- Mitochondrial Function and Pathology
- Advanced Proteomics Techniques and Applications
- RNA Research and Splicing
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- RNA regulation and disease
- Corneal Surgery and Treatments
- Cardiac Fibrosis and Remodeling
- Insect and Arachnid Ecology and Behavior
Sorbonne Université
2016-2025
Pitié-Salpêtrière Hospital
2013-2025
Inserm
2015-2023
Production et analyse de données en sciences de la vie et en santé
2020-2023
University of Liège
2022
Université Paris Cité
2015-2018
Sorbonne Paris Cité
2018
Institut des Neurosciences Paris-Saclay
2017
Digital Proteomics (United States)
2016
Institut de Biochimie et Biophysique Moléculaire et Cellulaire
2007-2014
The tetraspanins CD9, CD81 and CD63 are major components of extracellular vesicles (EVs). Yet, their impact on EV composition remains under-investigated. In the MCF7 breast cancer cell line was as expected predominantly intracellular. contrast CD9 strongly colocalized at plasma membrane, albeit with different ratios sites, which may explain a higher enrichment in EVs. Absence these had little protein analysed by quantitative mass spectrometry. We also effect concomitant knock-out because two...
The molecular basis of interindividual clinical variability upon infection with Staphylococcus aureus is unclear. We describe patients haploinsufficiency for the linear deubiquitinase OTULIN, encoded by a gene on chromosome 5p. Patients suffer from episodes life-threatening necrosis, typically triggered S. infection. disorder phenocopied in 5p- (Cri-du-Chat) chromosomal deletion syndrome. OTULIN causes an accumulation ubiquitin dermal fibroblasts, but tumor necrosis factor receptor-mediated...
Mechanosensitive channels are detected in all cells and speculated to play a key role many functions including osmoregulation, growth, hearing, balance, touch. In prokaryotic cells, direct gating of mechanosensitive by membrane tension was clearly demonstrated because the purified could be functionally reconstituted lipid bilayer. No such evidence has been presented yet case from animal cells. TREK-1, two-pore domain K(+) channel, first channel identified at molecular level. It is target...
Glutathionylation, the reversible post-translational formation of a mixed disulfide between cysteine residue and glutathione (GSH), is crucial mechanism for signal transduction regulation protein function. Until now this redox modification was studied mainly in eukaryotic cells. Here we report large-scale proteomic analysis glutathionylation photosynthetic prokaryote, model cyanobacterium Synechocystis sp. PCC6803. Treatment acellular extracts with N,N-biotinyl (BioGSSG) induced numerous...
Inflammatory bowel disease (IBD) results from a combination of genetic predisposition, dysbiosis the gut microbiota and environmental factors, leading to alterations in gastrointestinal immune response chronic inflammation. Caspase recruitment domain 9 (Card9), one IBD susceptibility genes, has been shown protect against intestinal inflammation fungal infection. However, cell types mechanisms involved CARD9 protective role remain unknown.We used dextran sulfate sodium (DSS)-induced adoptive...
Background: Courtship behavior plays a critical role in attracting females and reproduction success. However, the effects of exposure to ubiquitous contaminant di(2-ethylhexyl) phthalate (DEHP) on these behaviors and, particular, courtship vocalizations have not been examined. Objective: The adult DEHP mating gonadotropic axis neural mechanisms involved DEHP-induced were analyzed male mice. Methods: Adult C57BL/6J males orally exposed (0, 0.5, 5, 50μg/kg/d) for 4 wk. Olfactory preference,...
Hyper-activation of extracellular signal-regulated kinase (ERK) 1/2 contributes to heart dysfunction in cardiomyopathy caused by mutations the lamin A/C gene (LMNA cardiomyopathy). The mechanism how this affects cardiac function is unknown. We show that active phosphorylated ERK1/2 directly binds and catalyzes phosphorylation actin depolymerizing factor cofilin-1 on Thr25. Cofilin-1 becomes disassembles filaments a large array cellular animal models LMNA cardiomyopathy. In vivo expression...
In a continuing effort to analyze the selectivity/redundancy of three glutaredoxin (Grx) enzymes model cyanobacterium Synechocystis PCC6803, we have characterized an enzyme system that plays crucial role in protection against two toxic metal pollutants, mercury and uranium. The present data show Grx1 (Slr1562 CyanoBase) selectively interacts with presumptive mercuric reductase protein (Slr1849). This MerA cell defense both uranyl ions, catalyzing their NADPH-driven reduction. Like MerA,...
Background & AimsThe tumor-suppressor sterile α motif– and Src-homology 3–domain containing 1 (SASH1) has clinical relevance in colorectal carcinoma is associated specifically with metachronous metastasis. We sought to identify the molecular mechanisms linking decreased SASH1 expression distant metastasis formation.MethodsSASH1-deficient, SASH1-depleted, or SASH1-overexpressing HCT116 colon cancer cells were generated by Clustered Regularly Interspaced Short Palindromic...
Abstract There is currently no therapy to limit the development of cardiac fibrosis and consequent heart failure. We have recently shown that post-myocardial infarction (MI) can be regulated by resident cells with a fibrogenic signature identified expression PW1 ( Peg3 ). Here we identify αV-integrin (CD51) as an essential regulator + behavior. used transcriptomic proteomic approaches specific cell-surface markers for found was expressed in almost all (93% ± 1%), predominantly αVβ1 complex....
Pre-existing respiratory diseases may influence coronavirus disease (COVID-19) susceptibility and severity. However, the molecular mechanisms underlying airway epithelial response to severe acute syndrome 2 (SARS-CoV-2) infection severity in patients with chronic remain unelucidated. Using an vitro model of differentiated primary bronchial cells, we aimed investigate SARS-CoV-2 pre-existing cystic fibrosis (CF) obstructive pulmonary (COPD). Our study revealed reduced CF COPD epithelia...
Could the follicle proteome be mapped by identifying specific proteins that are common or differ between three developmental stages from secondary (SF) to antrum-like stage?
Sporozoites of the malaria parasite Plasmodium are transmitted by mosquitoes and infect liver for an initial obligatory round replication, before exponential multiplication in blood onset disease. stages provide attractive targets vaccines prophylactic drugs. In this context, defining proteome is important to explore biology identify potential antimalarial strategies. Previous studies have determined total sporozoites from two main human parasites, P. falciparum vivax, as well yoelii, which...
This study aimed to investigate the human proteome profile of samples collected from whole (W) Schirmer strips (ScS) and their two parts-the bulb (B) rest strip (R)-with a comprehensive proteomic approach using trapped ion mobility mass spectrometer, timsTOF Pro. Eight ScS were healthy subjects at four different visits be separated into three batches, i.e., 4W, 4B, 4R. In total, 1582 proteins identified in W, B, R batches. Among all proteins, binding (43.4%) those with catalytic activity...
Summary This study was conducted to establish a new methodology for evaluating elements of dermal extracellular matrix ( ECM ), epidermal–dermal junction EDJ and effects molecules which can modulate their synthesis. is based on matrix‐assisted laser desorption/ionization mass spectrometric imaging MALDI ‐ MSI ). In vivo reflectance confocal microscopy in RCM ) echography were also used. Using immunohistochemistry methods explants, age‐related modification data obtained selected proteins...
The nuclear proteome of Plasmodium falciparum results from the continual shuttle proteins between cell cytoplasm-nucleus and vice versa. Using shotgun proteomics tools, we explored mixed populations extracted infected erythrocytes. We combined GeLC-MS/MS 2D-LC-MS/MS with a peptide ion exclusion procedure in order to increase detection low abundant such as those involved gene expression. have identified 446 covering all expected protein families regulation. All structural ribosomal (40S 60S)...
Abstract The ocular surface (OS) enzymes are of great interest due to their potential for novel drug development. We aimed first profile and classify the OS describe major biological processes pathways that involved in maintenance homeostasis. Second, we compare enzymatic profiles between two most common tear collection methods, capillary tubes (CT) Schirmer strips (ScS). A comprehensive proteomic dataset was generated by pooling all identified from nine analyses healthy subjects using mass...