A5023623577- Circadian rhythm and melatonin
- Photoreceptor and optogenetics research
- Light effects on plants
- Pancreatic function and diabetes
- Genetics, Aging, and Longevity in Model Organisms
- Epigenetics and DNA Methylation
- Sleep and Wakefulness Research
- Spaceflight effects on biology
- Ubiquitin and proteasome pathways
- Neurobiology and Insect Physiology Research
- Protein Kinase Regulation and GTPase Signaling
- Receptor Mechanisms and Signaling
- Ion channel regulation and function
- Neuroendocrine regulation and behavior
- Genetics and Neurodevelopmental Disorders
- Neurogenesis and neuroplasticity mechanisms
- Fibroblast Growth Factor Research
- Congenital Diaphragmatic Hernia Studies
- Stress Responses and Cortisol
- RNA Interference and Gene Delivery
- Diabetes Management and Research
- Traditional Chinese Medicine Studies
- MicroRNA in disease regulation
- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
Jiaxing University
2025
Albert Einstein College of Medicine
2025
University of Toronto
2015-2024
Second Affiliated Hospital of Zhejiang University
2023
Capital Medical University
2006-2018
CM Hospital
2018
Guangdong Provincial People's Hospital
2015
University of Ottawa
2007-2014
Taizhou Municipal Hospital
2014
Yale University
2006-2013
Activity-regulated gene expression is believed to play a key role in the development and refinement of neuronal circuitry. Nevertheless, transcriptional networks that regulate synapse growth plasticity remain largely uncharacterized. Here, we show microRNA 132 (miR132) an activity-dependent rapid response regulated by cAMP element-binding (CREB) protein pathway. Introduction miR132 into hippocampal neurons enhanced dendrite morphogenesis whereas inhibition 2'O-methyl RNA antagonists blocked...
Mammalian circadian rhythms are synchronized to the external time by daily resetting of suprachiasmatic nucleus (SCN) in response light. As master pacemaker, SCN coordinates timing diverse cellular oscillators multiple tissues. Aberrant regulation clock is linked numerous human conditions, including cancer, cardiovascular disease, obesity, various neurological disorders and hereditary disorder familial advanced sleep phase syndrome. Additionally, mechanisms that underlie factor into...
To examine in vivo a rodent model the potential role of AMP-activated protein kinase (AMPK) within ventromedial hypothalamus (VMH) glucose sensing during hypoglycemia.Using gene silencing technology to selectively downregulate AMPK VMH, key hypothalamic glucose-sensing region, we demonstrate for detection hypoglycemia. In hyperinsulinemic-hypoglycemic (50 mg dl(-1)) clamp studies were performed awake, chronically catheterized Sprague-Dawley rats that had been microinjected bilaterally VMH...
The subgranular zone (SGZ) of the adult hippocampus contains a pool quiescent neural progenitor cells (QNPs) that are capable entering cell cycle and producing newborn neurons. mechanisms control timing extent neurogenesis not well understood. Here, we show QNPs SGZ express molecular-clock components proliferate in rhythmic fashion. clock proteins PERIOD2 BMAL1 critical for proper neurogenesis. absence abolishes gating cell-cycle entrance QNPs, whereas genetic ablation bmal1 results...
Histone variants were recently discovered to regulate neural plasticity, with H2A.Z emerging as a memory suppressor. Using whole-genome sequencing of the mouse hippocampus, we show that basal occupancy is positively associated steady-state transcription, whereas learning-induced removal gene expression. AAV-mediated depletion enhanced fear and resulted in gene-specific alterations reinforcing role accumulated age, although it remained sensitive eviction. Learning-related occurred at largely...
Cannabinoids modulate energy homeostasis and decrease cognitive arousal, possibly by acting on hypothalamic neurons including those that synthesize melanin-concentrating hormone (MCH) or hypocretin/orexin. Using patch-clamp recordings, we compared the actions of cannabinoid agonists antagonists identified MCH hypocretin in green fluorescent protein-expressing transgenic mice. The type-1 receptor (CB1R) agonist R -(+)-[2,3-dihydro-5-methyl-3-(4-morpho...
We have previously demonstrated that modulation of gamma-aminobutyric acid (GABA) inhibitory tone in the ventromedial hypothalamus (VMH), an important glucose-sensing region brain, modulates magnitude glucagon and sympathoadrenal responses to hypoglycemia. In current study, we examined whether increased VMH GABAergic may contribute suppression counterregulatory after recurrent hypoglycemia.To test this hypothesis, quantified expression GABA synthetic enzyme, glutamic decarboxylase (GAD),...
Defective counterregulatory responses (CRRs) to hypoglycemia are associated with a marked increase in the risk of severe hypoglycemia. The mechanisms leading development defective CRRs remain largely unknown, although they antecedent Activation AMP-activated protein kinase (AMPK) ventromedial hypothalamus (VMH) amplifies glucose production during acute To examine whether activation AMPK VMH restores CRR, controlled (∼2.8 mmol/l) was induced group 24 Sprague-Dawley rats, all which had...
The suprachiasmatic nucleus (SCN) acts as the central clock to coordinate circadian oscillations in mammalian behavior, physiology and gene expression. Despite our knowledge of transcriptome SCN, how it impacts genome-wide protein expression is not well understood. Here, we interrogated murine SCN proteome across cycle using SILAC-based quantitative mass spectrometry. Of 2112 proteins that were accurately quantified, 20% (421 proteins) displayed a time-of-day-dependent profile. Within this...
Significance Naked mole rats exhibit the most profound case of socially induced infertility among mammals. They live in sizable colonies where breeding is monopolized by two to four dominant animals; all other colony members retain prepubertal gonadal quiescence until death or removal from colony. Proximate mechanisms underlying this phenomenon are unknown but presumably involve integration environmental cues brain into neuroendocrine signals that impact gonadotropin-releasing hormone (GnRH)...
The central circadian pacemaker, the suprachiasmatic nucleus (SCN), encodes day length information by mechanisms that are not well understood. Here, we report genetic ablation of miR-132/212 alters entrainment to different lengths and non-24 hr day-night cycles, as photoperiodic regulation Period2 expression in SCN. SCN neurons from miR-132/212-deficient mice have significantly reduced dendritic spine density, along with altered methyl CpG-binding protein (MeCP2) rhythms. In Syrian hamsters,...
Signaling via the p42/44 mitogen-activated protein kinase (MAPK) pathway has been shown to be a key intracellular signaling event that couples light entrainment of mammalian circadian clock located in suprachiasmatic nucleus (SCN). Because many physiological effects MAPK are mediated by extracellular signal-regulated (ERK)-regulated kinases, it was interest identify targets ERK SCN. In this study, we examined whether mitogen- and stress-activated 1 (MSK1) is downstream target SCN gene...
Clock neurons within the mammalian suprachiasmatic nuclei (SCN) encode circadian time using interlocked transcription-translation feedback loops (TTFLs) that drive rhythmic gene expression. However, contributions of other transcription factors outside TTFLs to functionality SCN remain obscure. Here, we report stem and progenitor cell factor, sex-determining region Y-box 2 (SOX2), is expressed in adult positively regulates core clock gene, Period2. Mice lacking SOX2 selectively display...
Aging is associated with cognitive decline and dysregulation of the circadian system, which modulates hippocampal-dependent memory as well biological processes underlying hippocampal function. While dysfunction impairment are common features aging several neurodegenerative brain disorders, how impacts expression patterns proteins involved in that underlie not understood. In this study, we profiled proteomes young middle-aged mice across two cycles using quantitative mass spectrometry order...
Disruption of circadian rhythms during fetal development may predispose mice to developing heart disease later in life. Here, we report that male, but not female, had experienced chronic disturbance (CCD)
The mammalian master clock, located in the suprachiasmatic nucleus (SCN), is exquisitely sensitive to photic timing cues, but key molecular events that sculpt both phasing and magnitude of responsiveness are not understood. Here, we show Ras-like G-protein Dexras1 a critical factor these processes. -deficient mice ( dexras1 −/− ) exhibit restructured nighttime phase response curve loss gating resetting during day. affects sensitivity by repressing or activating time-of-day-specific signaling...