A5102739097- Connexins and lens biology
- Heat shock proteins research
- Plant-Microbe Interactions and Immunity
- Plant Stress Responses and Tolerance
- Urological Disorders and Treatments
- Urinary Bladder and Prostate Research
- Nicotinic Acetylcholine Receptors Study
- Genomics, phytochemicals, and oxidative stress
- Mercury impact and mitigation studies
- Legume Nitrogen Fixing Symbiosis
- PI3K/AKT/mTOR signaling in cancer
- Plant Parasitism and Resistance
- Heme Oxygenase-1 and Carbon Monoxide
- Neuroscience of respiration and sleep
- Calcium signaling and nucleotide metabolism
- Plant Pathogenic Bacteria Studies
- Fungal Biology and Applications
- Ion channel regulation and function
- NF-κB Signaling Pathways
- Redox biology and oxidative stress
- MicroRNA in disease regulation
- interferon and immune responses
- Photosynthetic Processes and Mechanisms
- Electrostatics and Colloid Interactions
- RNA regulation and disease
Duke University
2020-2023
Zhejiang University
2018-2021
Hangzhou Normal University
2021
Shenzhen University
2021
University of Yamanashi
2009-2016
Nanjing University of Chinese Medicine
2013
Plant nucleotide-binding leucine-rich repeat receptors (NLRs) regulate immunity and cell death. In
We investigated the potential involvement of connexin hemichannels in cadmium ions (Cd2+)-elicited cell injury. Transfection LLC-PK1 cells with a wild-type connexin43 (Cx43) sensitized them to Cd2+-elicited The susceptibility Cd2+ was increased by depletion glutathione (GSH) DL-buthionine-[S,R]-sulfoximine, and decreased N-acetyl-cysteine or reduced ethyl ester. Fibroblasts derived from Cx43 (Cx43+/+) knockout (Cx43-/-) fetal littermates displayed different Cd2+. induced higher concentration...
Flufenamic acid (FFA) is a nonsteroidal anti-inflammatory drug (NSAID). It has and antipyretic properties. In addition, it modulates multiple channel activities. The mechanisms underlying the pharmacological actions of FFA are presently unclear. Given that AMP-activated protein kinase (AMPK) both channel-regulating functions, we examined whether induces AMPK activation. 1) Exposure several different types cells to resulted in an elevation AMPKα phosphorylation at Thr172. This effect was...
Oxidative stress–induced podocyte injury is one of the major mechanisms underlying initiation and progression glomerulosclerosis. 5′-AMP-activated protein kinase (AMPK), a serine/threonine that senses intracellular energy status maintains homeostasis, reported to have antioxidative effects. However, little known about its application mechanism. In this study, we investigated whether how AMPK affected oxidative induced by Adriamycin (ADR; Wako Pure Chemical, Osaka, Japan). Exposure podocytes...
Connexin hemichannels regulate many cell functions. However, the molecular mechanisms involved remain elusive. Given that hemichannel opening causes loss of ATP, we therefore speculated a potential implication AMPK in biological actions hemichannels. Activation by removing extracellular Ca2+ led to an efflux ATP and weak activation AMPK. Unexpectedly, dysfunction markedly potentiated activation, which was reproduced promotion degradation or inhibition P2 purinoceptors, but counteracted...
Gap junctions (GJs) play an important role in the control of bladder contractile response and regulation various immune inflammatory processes. Here, we investigated possible interaction between inflammation GJs smooth muscle cells (BSMCs). Stimulation BSMCs with IL1β TNFα increased connexin43 (Cx43) expression function, which was associated phosphorylation vasodilator-stimulated phosphoprotein. Inhibition PKA H89 or down-regulation CREB specific siRNAs largely abolished Cx43-elevating...
Abstract The flagellin epitope flg22, a pathogen‐associated molecular pattern (PAMP), binds to the receptor‐like kinase FLAGELLIN SENSING2 (FLS2), and triggers Ca 2+ influx across plasma membrane (PM). flg22‐induced increases in cytosolic concentration ([Ca ] i ) (FICA) play crucial role plant innate immunity. It's well established that receptor FLS2 reactive oxygen species (ROS) burst undergo sensitivity adaptation after flg22 stimulation, referred as desensitization resensitization,...
Many signaling molecules and pathways that regulate gap junctions (GJs) protein expression function are, in fact, also controlled by GJs. We, therefore, speculated an existence of the GJ channel-mediated self-regulation Using a cell culture model which nonjunctional connexin43 (Cx43) hemichannels were activated cadmium (Cd(2+)), we tested this hypothesis.Incubation Cx43-transfected LLC-PK1 cells with Cd(2+) led to increased Cx43. This effect was tightly associated JNK activation. Inhibition...
Proteasome inhibition is a promising approach for cancer therapy. However, the mechanisms involved have not been fully elucidated. Gap junctions play important roles in regulation of tumor cell phenotypes and mediation bystander effect Because degradation gap junction proteins involves proteasome, we speculated that altered might contribute to antitumor activities proteasome inhibition. Incubation Hepa-1c1c7 cells with inhibitor MG132 elevated levels protein connexin 43 (Cx43) promoted...
Cordycepin (3'-deoxyadenosine) is one of the major bioactive substances produced by Cordyceps militaris, a traditional medicinal mushroom. possesses several biological activities, including both pro-apoptotic and anti-apoptotic properties. In present report, we investigated an effect cordycepin on survival cells exposed to tumour necrosis factor (TNF)-α. We found that subtoxic doses increased susceptibility TNF-α-induced apoptosis. It was associated with suppression nuclear factor-κB...
Abstract Bladder voiding dysfunction is closely related to local oxidation, inflammation and enhanced channel activities. Given that the AMP-activated protein kinase (AMPK) has anti-oxidative, anti-inflammatory channel-inhibiting properties, we examined whether how AMPK affected bladder activity. activation in rat smooth muscle cells (BSMCs) using three different agonists resulted a decrease connexin43 (Cx43) expression function, which was associated with reduced CREB phosphorylation, Cx43...
Background and Purpose Suramin is a clinically prescribed drug for treatment of human A frican trypanosomiasis, cancer infection. It also well‐known pharmacological antagonist P 2 purinoceptors. Despite its clinical use in research, the biological actions this molecule are still incompletely understood. Here, we investigated effects suramin on membrane channels, as exemplified by non‐junctional connexin43 ( C x43) hemichannels, pore‐forming α‐haemolysin channels involved ATP release under...
Salubrinal is a selective inhibitor of cellular complexes that dephosphorylate eukaryotic translation initiation factor 2α (eIF2α). In previous reports, salubrinal was shown to have the potential inhibit activation nuclear factor-κB (NF-κB) by several stimuli. However, effects on NF-κB signaling are largely unknown. this study, we investigated whether and how affects induced tumor necrosis (TNF)-α interleukin (IL)-1β. We found selectively blocked TNF-α- but not IL-1β-induced NF-κB. This...
Gap junctions play important roles in the regulation of cell phenotype and determining survival after various insults. Here, we investigated role gap aminoglycoside-induced injury to renal tubular cells.Two epithelial lines NRK-E52 LLC-PK1 were compared for junction protein expression function by immunofluorescent staining, Western blot dye transfer assay. Cell viability exposure aminoglycosides was evaluated WST modulated transfection protein, connexin 43 (Cx43), use Cx43 siRNA...
Abstract Gap junctions ( GJ s) play an important role in the regulation of cell response to many drugs. However, little is known about their mechanisms. Using vitro model cytotoxicity induced by geneticin (G418), we explored potential signalling mechanisms involved. Incubation cells with G418 resulted death, as indicated change morphology, loss viability and activation caspase‐3. Before onset injury, reactive oxygen species ROS ) generation, activated oxidative sensitive kinase P38 caused a...
Abstract Whiteflies ( Bemisia tabaci ) are phloem feeders, and some invasive species composed of cryptic complexes that cause extensive crop damage, particularly via the direct transmission plant viruses. Apoptosis is a type programmed cell death essential for organismal development tissue homeostasis. The caspases belong to family cysteine proteases play central role in initiation apoptosis many organisms. Here, we employed comprehensive genomics approach identity B. Middle East Asia Minor...
Eviprostat is a popular phytotherapeutic agent for the treatment of lower urinary tract symptoms (LUTS). At present, signaling mechanisms underlying its therapeutic effects are still poorly understood. Given that cAMP has been reported to suppress cell hyperplasia and hypertrophy in various pathological situations, we asked whether effect could be ascribed activation pathway. In study, exposure response element (CRE)-secreted alkaline phosphatase (SEAP) (CRE-SEAP)-reporter cells elevated...
Thioredoxin (Trx) is a small redox protein that underlies aggressive tumor growth and resistance to chemotherapy. Inhibition of Trx with the chemical inhibitor PX-12 suppresses induces cell apoptosis. Currently, mechanism underlying therapeutic actions molecules influencing susceptibility are incompletely understood. Given connexin43 (Cx43), suppressor, regulates chemotherapy, we examined possible involvement Cx43 in PX-12-induced death. Exposure cells led loss viability, which was...
SUMMARY ACCELERATED CELL DEATH 6 (ACD6) mediates a trade-off between growth and defense in Arabidopsis thaliana . However, the precise biochemical mechanism by which ACD6 related proteins plants act remains enigmatic. Here, we identified two loci, MODULATOR OF HYPERACTIVE 1 ( MHA1 ) its paralog MHA1-LIKE MHA1L ), that code for ∼7 kDa differentially interact with specific variants. enhances accumulation of an complex, thereby increasing activity standard allele regulating plant defenses. is...
The flagellin epitope flg22, a pathogen-associated molecular pattern (PAMP), binds to the receptor-like kinase FLAGELLIN SENSING2 (FLS2), and triggers Ca2+ influx across plasma membrane (PM). flg22-induced increases in cytosolic concentration ([Ca2+]i) (FICA) play crucial role plant innate immunity. It’s well established that receptor FLS2 key downstream component, reactive oxygen species (ROS) burst, undergoes sensitivity adaptation after flg22 stimulation, referred as desensitization...
Connexin (Cx) hemichannels regulate many cellular processes with little information available regarding their mechanisms. Given that pathological factors activate also disrupt the integrity of junctions, we speculated a potential participation in regulation dissemble tight/adherent junctions (TJs/AJs). Here tested this hypothesis. 1) Exposure renal tubular epithelial cells to Ca 2+ ‐free medium led disassembly TJs and AJs, as indicated by reduced ZO‐1 E‐cadherin, disorganized F‐actin, well...