A5033713077- Ion Channels and Receptors
- Adenosine and Purinergic Signaling
- Sulfur Compounds in Biology
- COVID-19 Clinical Research Studies
- Redox biology and oxidative stress
- SARS-CoV-2 and COVID-19 Research
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Receptor Mechanisms and Signaling
- Amino Acid Enzymes and Metabolism
- Muscle metabolism and nutrition
- Chemotherapy-induced cardiotoxicity and mitigation
- Coenzyme Q10 studies and effects
- Electron Spin Resonance Studies
- Cancer therapeutics and mechanisms
- Pharmacological Receptor Mechanisms and Effects
- Endoplasmic Reticulum Stress and Disease
- Hormonal Regulation and Hypertension
- Vitamin C and Antioxidants Research
- RNA Interference and Gene Delivery
- Cardiovascular Function and Risk Factors
- Liver Disease Diagnosis and Treatment
- Pancreatic function and diabetes
- Neuropeptides and Animal Physiology
Kyushu University
2020-2025
University of Tsukuba
2023-2024
Advanced Science Research Center
2017-2022
Okayama University
2013-2022
Okayama University of Science
2015-2020
Despite the high incidence of neuropathic and inflammatory pain worldwide, effective drugs with few side effects are currently unavailable for treatment chronic pain. Recently, researchers have proposed that inhibitors purinergic chemical transmission, which plays a key role in pathological response, may allow targeted However, such therapeutic analgesic agents yet to be developed. In present study, we demonstrated clodronate, first-generation bisphosphonate comparatively fewer than...
Abstract About 60–85% of total phosphorus (P) in cereal crops is finally allocated to seeds, where it required for seed development, germination and early growth. However, little known about the molecular mechanisms underlying P allocation seeds. Here, we found that two members (OsPHO1;1 OsPHO1;2) PHO1 gene family are involved distribution seeds rice. Both OsPHO1;1 OsPHO1;2 were localized plasma membrane showed influx transport activities inorganic phosphate. At reproductive stage, both...
Abstract The presence of redox-active molecules containing catenated sulfur atoms (supersulfides) in living organisms has led to a review the concepts redox biology and its translational strategy. Glutathione (GSH) is body’s primary detoxifier antioxidant, oxidized form (GSSG) been considered as marker oxidative status. However, we report that GSSG, but not reduced GSH, prevents ischemic supersulfide catabolism-associated heart failure male mice by electrophilic modification dynamin-related...
Abnormal sulfide catabolism, especially the accumulation of hydrogen (H2S) during hypoxic or inflammatory stresses, is a major cause redox imbalance-associated cardiac dysfunction. Polyhydroxynaphtoquinone echinochrome A (Ech-A), natural pigment marine origin found in shells and needles many species sea urchins, potent antioxidant inhibits acute myocardial ferroptosis after ischemia/reperfusion, but chronic effect Ech-A on heart failure unknown. Reactive sulfur (RSS), which include catenated...
Sulfur-based redox signaling has long attracted attention as critical mechanisms underlying the development of cardiac diseases and resultant heart failure. Especially, post-translational modifications cysteine (Cys) thiols in proteins mediate oxidative stress-dependent remodeling including myocardial hypertrophy, senescence, interstitial fibrosis. However, we recently revealed existence Cys persulfides polysulfides cells tissues, which show higher activities than substantially contribute to...
Cysteine modification of the purinergic receptor P2Y 6 R promotes internalization and degradation limits colitis progression.
The newly emerged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is causing a spread of disease 2019 (COVID-19) globally. In order to end the COVID-19 pandemic, an effective vaccine against SARS-CoV-2 must be produced at low cost and disseminated worldwide. spike (S) protein coronaviruses plays pivotal role in infection host cells. Therefore, targeting S one most rational approaches developing vaccines therapeutic agents. this study, we optimized expression secreted trimerized...
Lipid droplet (LD) accumulation in hepatocytes is one of the major symptoms associated with fatty liver disease. Mitochondria play a key role catabolizing acids for energy production through β-oxidation. The interplay between mitochondria and LD assumes crucial lipid metabolism, while it obscure how mitochondrial morphology affects systemic metabolism liver. We previously reported that cilnidipine, an already existing anti-hypertensive drug, has potency to prevent pathological fission by...
Lipid droplet (LD) accumulation in hepatocytes is one of the major symptoms associated with fatty liver disease. Mitochondria play a key role catabolizing acids for energy production through β-oxidation. The interplay between mitochondria and LD assumes crucial lipid metabolism, while it obscure how mitochondrial morphology affects systemic metabolism liver. We previously reported that cilnidipine, an already existing anti-hypertensive drug, can prevent pathological fission by inhibiting...
Abstract Baroreflex control of cardiac contraction (positive inotropy) through sympathetic nerve activation is important for cardiocirculatory homeostasis. Transient receptor potential canonical subfamily (TRPC) channels are responsible α 1 -adrenoceptor (α AR)-stimulated cation entry and their upregulation associated with pathological remodeling. Whether TRPC participate in physiological pump functions remains unclear. We demonstrate that TRPC6-specific Zn 2+ influx potentiates...
Introduction We aimed to determine the effects of exercise on cell-free DNA (cfDNA) levels and concentration changes during menstrual cycle in participants with regular cycles no habits. Methods Eleven sedentary female students ovulation performed bicycle exercises at 60% VO 2max for 30 min menstrual, ovulatory, luteal phases. Blood samples were collected before (Pre), immediately after (Post 0), 30), 60 60) exercise. concentrations ovarian hormones, cfDNA, prostaglandin F2a (PGF2α),...
Maintaining mitochondrial quality is attracting attention as a new strategy to treat diabetes and diabetic complications. We previously reported that hyperfission by forming protein complex between dynamin-related (Drp) 1 filamin, mediates chronic heart failure cilnidipine, initially developed an L/N-type Ca
G protein-coupled receptors (GPCRs) exist in the conformational equilibrium between inactive state and active state, where proportion of absence a ligand determines basal activity GPCRs. Although many GPCRs have different activity, it is still unclear whether physiological stresses such as substrate stiffness affect In this study, we identified that purinergic P2Y6 receptor (P2Y6R) induced spontaneous Ca2+ oscillation without nucleotide when cells were cultured silicon chamber. This...
Vascular smooth muscle cells (VSMCs) can dynamically change their phenotype between contractile and synthetic forms in response to environmental stress, which is pivotal maintaining vascular homeostasis mediating pathological remodeling of blood vessels. We previously reported that suppression canonical transient receptor potential 6 (TRPC6) channel-mediated cation entry sustains VSMCs promotes the flow recovery after hindlimb ischemia mice. also Zn2+, a metal biomolecule mobilized by TRPC6...
Neutrophils migrate to sites infected by pathogenic microorganisms. This migration is regulated neutrophil-secreted ATP, which stimulates neutrophils in an autocrine manner through purinergic receptors on the plasma membrane. Although previous studies have shown that ATP released channels at membrane of neutrophil, it remains unknown whether also alternate secretory systems involving vesicular mechanisms. In this study, we investigated possible involvement nucleotide transporter (VNUT), a...
Smoking is one of the most serious risk factors for cardiovascular diseases. Although cigarette mainstream and sidestream smoke are significant contributors to increased mortality morbidity, underlying mechanism still unclear. Here, we report that exposure rat neonatal cardiomyocytes extract (CSE) induces mitochondrial hyperfission-mediated myocardial senescence. CSE leads fission reactive oxygen species (ROS) production through complex formation between factor Drp1 actin-binding protein,...
Abstract Remdesivir is an antiviral drug used for COVID-19 treatment worldwide. Cardiovascular side effects have been associated with remdesivir; however, the underlying molecular mechanism remains unknown. Here, we performed a large-scale G-protein-coupled receptor screening in combination structural modeling and found that remdesivir selective, partial agonist urotensin-II (UTS2R) through Gα i/o -dependent AKT/ERK axis. Functionally, induced prolonged field potential APD 90 human...
Despite the widespread recognition of global concern regarding onset cardiovascular diseases in a significant number patients following cancer treatment, definitive strategies for prevention and treatment remain elusive. In this study, we established systems to evaluate influence anti-cancer drugs on quality control mitochondria, pivotal energy metabolism, using human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Osimertinib, an epidermal growth factor receptor tyrosine...
Abstract Cardiac tissue remodeling caused by hemodynamic overload is a major clinical outcome of heart failure. Uridine-responsive purinergic P2Y 6 receptor (P2Y R) contributes to the progression cardiovascular in rodents, but it not known whether inhibition R prevents or promotes We demonstrate that pressure overload-induced sudden death and failure mice. In neonatal cardiomyocytes, knockdown significantly attenuated hypertrophic growth cell hypotonic stimulation, indicating involvement...
Eicosapentaenoic acid (EPA), an omega-3 (ω-3) polyunsaturated fatty acid, is essential nutrient that exhibits antiinflammatory, neuroprotective, and cardiovascular-protective activities. Although EPA used as a nutrient-based pharmaceutical agent or dietary supplement, its molecular target(s) debatable. Here, we showed metabolites strongly reversibly inhibit vesicular nucleotide transporter (VNUT), key molecule for storage release of adenosine triphosphate (ATP) in purinergic chemical...
Vesicular nucleotide transporter (VNUT) is responsible for vesicular ATP storage in ATP-secreting cells. In the present study, we examined effects on VNUT-mediated transport of release inhibitors such as ATP-binding cassette (ABC) proteins, hemichannels, maxi anion channels and P2X7 receptor. The activity proteoliposomes containing purified human VNUT was blocked by glibenclamide, carbenoxolone, 18 α-glycyrrhetinic acid, flufenamic arachidonic acid A438079 without formation Δψ (positive...
Nonalcoholic steatohepatitis (NASH) is a disease that progresses from nonalcoholic fatty liver (NAFL) and which characterized by inflammation fibrosis. The purinergic P2Y6 receptor (P2Y6R) pro-inflammatory Gq/G12 family protein-coupled reportedly contributes to intestinal cardiovascular fibrosis, but its role in pathogenesis unknown. Human genomics data analysis revealed the P2Y6R mRNA expression level increased during progression NAFL NASH, positively correlates with inductions of C-C motif...