A5014430974- Viral Infectious Diseases and Gene Expression in Insects
- Insect Resistance and Genetics
- Invertebrate Immune Response Mechanisms
- Silk-based biomaterials and applications
- Glycosylation and Glycoproteins Research
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Silkworms and Sericulture Research
- Transgenic Plants and Applications
- Neurobiology and Insect Physiology Research
- Insect symbiosis and bacterial influences
- Building materials and conservation
- Autophagy in Disease and Therapy
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Studies on Chitinases and Chitosanases
- Monoclonal and Polyclonal Antibodies Research
- Insect Utilization and Effects
- Insect and Pesticide Research
- Viral gastroenteritis research and epidemiology
- Epigenetics and DNA Methylation
- Pancreatic function and diabetes
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
Kyushu University
2016-2025
Kyungpook National University
2016-2025
Yeungnam University
2023
Matrix Research (United States)
2017
Baylor College of Medicine
2011-2016
Chung-Ang University
2016
National Research Institute of Cultural Heritage
2012-2014
The Dainippon Silk Foundation
2010
CHA Bundang Medical Center
2010
CHA University
2010
Abstract Inhibitors of the secretion cancer exosomes, which promote progression and metastasis, may not only accelerate exosome biology research but also offer therapeutic benefits for patients. Here we identify sulfisoxazole (SFX) as an inhibitor small extracellular vesicles (sEV) from breast cells through interference with endothelin receptor A (ETA). SFX, FDA-approved oral antibiotic, showed significant anti-tumor anti-metastatic effects in mouse models xenografts, reduced expression...
Abstract Degenerative disorders of the intervertebral discs (IVDs) are generally characterized by enhanced matrix degradation, angiogenesis, innervation, and increased expression catabolic cytokines. In this study, we investigated effects inflammatory cytokines, IL‐1β, TNF‐α, on an angiogenic factor, vascular endothelial growth factor (VEGF), neurotrophic factors, nerve (NGF) brain‐derived (BDNF), in human IVD degeneration. IL‐1β TNF‐α stimulated gene VEGF, NGF, BDNF nucleus pulposus (NP)...
The nuclear receptors farnesoid X receptor (FXR; NR1H4) and small heterodimer partner (SHP; NR0B2) play crucial roles in bile acid homeostasis. Global double knockout of FXR SHP signaling (DKO) causes severe cholestasis liver injury at early ages. Here, we report an unexpected beneficial impact on glucose fatty metabolism aged DKO mice, which show suppressed body weight gain adiposity when maintained normal chow. This phenotype was not observed single Fxr or Shp knockouts. Liver-specific...
Although macroautophagy/autophagy deficiency causes degenerative diseases, the deletion of essential autophagy genes in adipocytes paradoxically reduces body weight. Brown adipose tissue (BAT) plays an important role weight regulation and metabolic control. However, key cellular mechanisms that maintain BAT function remain poorly understood. this study, we showed global or brown adipocyte-specific pink1, a Parkinson disease-related gene involved selective mitochondrial (mitophagy), induced...
•In models of cholestasis, autophagy is impaired at late levels, when autophagosomes and lysosomes would normally fuse.•The impairment depends on bile acids FXR-dependent induction Rubicon.•OCA impairs autophagy, while UDCA a potent activator hepatic autophagy.•Autophagy, in particular Rubicon, represents novel molecular drug target cholestatic liver diseases.•UDCA may already be used diseases where warranted. Background & AimsCholestasis comprises spectrum characterized by the accumulation...
Chronic endoplasmic reticulum (ER) stress results in toxicity that contributes to multiple human disorders. We report a resolution pathway initiated by the nuclear receptor LRH-1 is independent of known unfolded protein response (UPR) pathways. Like mice lacking primary UPR components, hepatic Lrh-1-null cannot resolve ER stress, despite functional UPR. In induces expression kinase Plk3, which phosphorylates and activates transcription factor ATF2. Plk3-null also restoring Plk3 cells rescues...
Abstract Gene targeting can be achieved by precise genetic modifications through homology-directed repair (HDR) after DNA breaks introduced genome editing tools such as CRISPR/Cas9 system. The most common form of HDR is homologous recombination (HR). Binding to the HR factors thought compete with non-homologous end joining (NHEJ), an alternative pathway. Here, we knocked out in NHEJ system silkworm cells, so that increased activities up 7-fold. Also efficient HR-mediated events occurred...
In mammalian striated muscles, ryanodine receptor (RyR), triadin, junctin, and calsequestrin form a quaternary complex in the lumen of sarcoplasmic reticulum. Such intermolecular interactions contribute not only to passive buffering reticulum luminal Ca2+, but also active Ca2+ release process during excitation-contraction coupling. Here we tested hypothesis that specific charged amino acids within portion RyR mediate its direct interaction with triadin. Using vitro binding assay...
Mixed background SHP(-/-) mice are resistant to diet-induced obesity due increased energy expenditure caused by enhanced PGC-1α expression in brown adipocytes. However, congenic on the C57BL/6 showed normal of and other genes involved adipose tissue thermogenesis. Thus, we reinvestigated impact small heterodimer partner (SHP) deletion insulin resistance using mice. Compared with their wild-type counterparts, subjected a 6 month challenge Western diet (WestD) were leaner but more glucose...
Calsequestrin (CSQ) is a high capacity Ca<sup>2+</sup>-binding protein present in the lumen of sarcoplasmic reticulum (SR) striated muscle cells and has been shown to regulate ryanodine receptor Ca<sup>2+</sup> release channel activity through interaction with other proteins SR. Here we show that overexpression wild-type CSQ or mutant lacking junction binding region (amino acids 86–191; Δ<i>junc</i>-CSQ) mouse skeletal C2C12 myotube enhanced caffeine- voltage-induced by increasing load SR,...
Although the main cause of degeneration nigrostriatal dopaminergic (DA) projection in Parkinson's disease (PD) is still controversial, many reports suggest that excessive inflammatory responses mediated by activated microglia can induce neurotoxicity DA system vivo. Montelukast, which plays an anti-inflammatory role, used to treat patients with asthma. In addition, recent studies have reported its administration could reduce neuroinflammatory activities, showing beneficial effects against...
Liver fibrosis is a reversible wound-healing process that protective in the short term, but prolonged fibrotic responses lead to excessive accumulation of extracellular matrix components suppresses hepatocyte regeneration, resulting permanent liver damage. Upon damage, nonparenchymal cells including immune and hepatic stellate (HSCs) have crucial roles progression regression fibrosis. Here, we report differential glucocorticoid receptor (GR), acting HSCs, In carbon tetrachloride...
The newly emerged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is causing a spread of disease 2019 (COVID-19) globally. In order to end the COVID-19 pandemic, an effective vaccine against SARS-CoV-2 must be produced at low cost and disseminated worldwide. spike (S) protein coronaviruses plays pivotal role in infection host cells. Therefore, targeting S one most rational approaches developing vaccines therapeutic agents. this study, we optimized expression secreted trimerized...
To investigate the role of superoxide dismutase (SOD) in virulence against silkworm, Bombyx mori, mutants Pseudomonas aeruginosa PAO1 lacking manganese-SOD (PAO1sodM), iron-SOD (PAO1sodB), or both (PAO1sodMB) were generated. The injected into hemocoel B. mori. decreased order PAO1=PAO1sodM>PAO1sodB>PAO1sodMB. In particular, PAO1sodMB was avirulent at a dose 10(5) cells less. sod double mutant then complemented with either pSodM pSodB trans. strains, partially restored. Of two plasmids,...