A5045599016- Sarcoma Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- Retinal Development and Disorders
- Fluid Dynamics and Turbulent Flows
- Aerodynamics and Fluid Dynamics Research
- Cancer Cells and Metastasis
- Neuroblastoma Research and Treatments
- Fluid Dynamics and Vibration Analysis
- Computational Fluid Dynamics and Aerodynamics
- Retinal Diseases and Treatments
- Gastrointestinal disorders and treatments
- Epigenetics and DNA Methylation
- Vascular Procedures and Complications
- Renal and related cancers
- Glaucoma and retinal disorders
- Cancer-related gene regulation
- Gas Dynamics and Kinetic Theory
- Aortic aneurysm repair treatments
- Immune Cell Function and Interaction
- Colorectal and Anal Carcinomas
- Mesenchymal stem cell research
- CAR-T cell therapy research
- Cancer therapeutics and mechanisms
- Molecular Biology Techniques and Applications
- Diverticular Disease and Complications
University of Colorado Denver
2020-2025
Children's Hospital Colorado
2020-2025
University of Colorado Anschutz Medical Campus
2020-2025
Keio University
2013-2024
Shuto General Hospital
2019-2024
Center for Cancer and Blood Disorders
2020-2024
University of Colorado Cancer Center
2024
Kishiwada Tokushukai Hospital
2024
Saiseikai Central Hospital
2023
University of Colorado Hospital
2023
Recent studies have shown that bone marrow-derived stem cells differentiate into the phenotype of cardiomyocytes in vivo and vitro. We tried to regenerate infarcted myocardium by implanting ex transforming growth factor (TGF)-beta-preprogrammed CD117 (c-kit)-positive (CD117+) intramyocardially.CD117+ were isolated from marrow mononuclear GFP-transgenic or normal C57/BL6 mice. The myogenic differentiation CD117+ was achieved cultivation with TGF-beta. Using an acute myocardial infarction...
Maize (Zea mays L. cv Golden Cross Bantam T51) seedlings were grown under full sunlight or 50% in a temperature-controlled glasshouse at the temperatures of near optimum (30/25 degrees C) and suboptimum (17/13 with seven levels nitrate-N (0.4 to 12 millimolars). The contents phosphoenolpyruvate carboxylase (PEPC), pyruvate orthophosphate dikinase (PPD), ribulose-1,5-P(2) carboxylase/oxygenase (RuBisCO) immunochemically determined for each treatment rabbit antibodies raised against respective...
The outcome of patients with metastatic osteosarcoma has not improved since the introduction chemotherapy in 1970s. Development therapies targeting cascade is a tremendous unmet medical need. Wnt signaling pathway been focus intense investigation because its role normal bone development. Although pathogenesis controversial, there are several reports dickkopf-1 (DKK-1), antagonist, possibly playing pro-tumorigenic role. In this work we investigated effect anti-DKK-1 antibodies on growth and...
Even though virtually all patients with Ewing sarcoma achieve a radiographic complete response, up to 30% of who present localized disease and 90% those metastases experience metastatic recurrence, highlighting the inability identify residual at end therapy. Up 95% sarcomas carry driving EWS-ETS translocation that has an intronic breakpoint is specific each tumor, authors developed system quantitatively detect DNA fragment in patient plasma.The used long-range multiplex polymerase chain...
Wingless (Wnt) signaling is an important pathway in gliomagenesis and the growth of stem-like glioma cells. Using immunohistochemistry to assess translocation β-catenin protein, we identified intranuclear staining suggesting Wnt activation 8 43 surgical samples (19%) from adult patients with glioblastoma 9 30 (30%) pediatric glioblastoma. activity, evidenced by nuclear our cohort high expression its target AXIN2 (axis inhibitor protein 2) published datasets, was associated shorter patient...
Therapeutic angiogenesis can be induced by the implantation of bone marrow mononuclear cells. We investigated roles mature cell and stem fractions in this treatment. Although CD34 is most popular marker for selection inducing therapeutic angiogenesis, we separated CD117-positive cells (CD117 + ) from [CD117-negative – )] mice using antibody to receptor, because some that express CD117 might generate angiogenic cytokines differentiate into endothelial The potency was vitro vivo. Significantly...
Metastatic disease is the most important factor in determining survival of sarcoma patients. Since metastasis predominantly hematogenous, we hypothesized that detection and quantification circulating tumor cells (CTCs) could reflect response to therapy risk metastatic relapse. We evaluated presence CTCs using a novel animal model blood patients with high grade sarcomas utilizing CellSieve™ size-based low pressure microfiltration system. Sarcoma were identified based on antibody staining...
Lessons from extinction can be used in trials designed to pursue a cure for cancer. When cancer cannot cured, similar strategies may unwise, and that leverage the adaptations of therapy should considered.
NR2E3, a photoreceptor-specific nuclear receptor (PNR), represses cone-specific genes and activates several rod-specific genes. In humans, mutations in NR2E3 have been associated with the recessively-inherited enhanced short-wavelength sensitive S-cone syndrome (ESCS) and, recently, autosomal dominant (ad) retinitis pigmentosa (RP) (adRP). present work, we describe two additional families affected by adRP that carry heterozygous c.166G>A (p.G56R) mutation gene. Functional analysis determined...
Efficient one-pot synthesis in water of polyrotaxanes, with native and permethylated α-cyclodextrins (α-CD PMe α-CD) as the wheel components, is described. The procedure involves initial mixing α-CDs an amine-terminated linear polymer, which acts axle, by sonication subsequent addition end-capping agent for axle terminal amino groups. polyrotaxanes were prepared components 30 min at room temperature standing overnight if necessary, followed treatment a bulky isocyanate 0 °C hour. Both...
The wake characteristic of groups normal flat plates, consisting two, three, or four plates placed side by with slits in between, have been investigated experimentally. When the ratio slit width to plate (slit ratio) was small, gap flows were observed be biased either upward downward a stable way, leading multiple, flow patterns for single slit-ratio value. Some regularities recognized gap-flow directions and appearance patterns. on showed high drag regular vortex shedding, while those...
Abstract For solid tumors, extravasation of cancer cells and their survival in circulation represents a critical stage the metastatic process that lacks complete understanding. Gaining insight into interactions between circulating tumor (CTCs) other peripheral blood mononuclear (PBMCs) may provide valuable prognostic information. The purpose this study was to use single‐cell RNA‐sequencing (scRNA‐seq) liquid biopsies from breast patients begin defining intravascular interactions. We captured...
Background: Sarcomas are a heterogeneous group of mesenchymal tumors frequently diagnosed in pediatric and young adult patients. These respond poorly to conventional immunotherapy, though the precise reason for this is not known. We sought characterize systemic immune response sarcomas by measuring levels circulating cytokines plasma sarcoma patients, testing hypothesis that natures patient's their tumor directly affects outcome. Methods: Plasma was collected from newly diagnosed,...
// Masanori Hayashi 1 , Alissa Baker Seth D. Goldstein Catherine M. Albert 1,3 Kyle W. Jackson Gregory McCarty Ulf Kahlert 1,2 and David Loeb Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD, USA 2 Neurosurgical Clinic, University Medical Center Düsseldorf, Germany 3 Seattle Children's Hospital, of Washington, Seattle, WA, Correspondence to: Loeb, email: Keywords : metastasis, patient-derived xenograft, preclinical model, Wnt signaling, Ewing sarcoma...
11519 Background: Recurrent ES carries a poor prognosis, and systemic therapies have limited efficacy. Preclinical models suggested that trabectedin (T) could achieve serum concentrations high enough to suppress the dominant oncogene of (i.e. EWS::FLI1 transcription factor), this effect is sustained by subsequent administration low dose irinotecan (I). We conducted phase I study T+I in patients (pts) with ES. Methods: This multicenter escalation employed standard 3+3 design. T was given as...
Abstract To date, few studies have detailed the tumor microenvironment (TME) of Ewing sarcoma (EwS). The TME has a vital role in cancer survival and progression with implications drug resistance immune escape. By performing spatially resolved transcriptomic analysis primary treatment-naïve EwS samples, we discovered greater stromal enrichment localized tumors compared to metastatic tumors. Through spatial ligand-receptor analysis, show that enriched regions harbor unique extracellular matrix...
Abstract Purpose: Ewing sarcoma (EwS) is the second most common bone cancer in children, accounting for 2% of pediatric diagnoses. Patients who present with metastatic disease at time diagnosis have a dismal prognosis, compared to >70% 5-year survival those localized disease. Novel therapeutic approaches that can impact are desperately needed, as well deeper understanding heterogeneity EwS tumors. Experimental Design: Here, we utilized single cell RNA-sequencing characterize...
Abstract Osteosarcoma is the most common bone cancer in children, adolescent and young adults. Recent large scale genomic studies have started to reveal that (OS) patients who tumors highly express transcription factor MYC, are significantly at high risk of treatment failure. However, MYC has long been known be extremely difficult target with drugs. The EYA protein an evolutionarily conserved transcriptional co-activator SIX1 family homeobox proteins, which also phosphatase activity....