A5046023135- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Biosensors and Analytical Detection
- Catalytic Processes in Materials Science
- Cancer-related molecular mechanisms research
- Catalysts for Methane Reforming
- Catalysis and Hydrodesulfurization Studies
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Advanced Nanomaterials in Catalysis
- RNA modifications and cancer
- DNA and Nucleic Acid Chemistry
- Catalysis for Biomass Conversion
- Circular RNAs in diseases
- Nanocluster Synthesis and Applications
- Molecular Sensors and Ion Detection
- interferon and immune responses
- CRISPR and Genetic Engineering
- Biodiesel Production and Applications
- Photochromic and Fluorescence Chemistry
- Luminescence and Fluorescent Materials
- Advanced Biosensing Techniques and Applications
- Conducting polymers and applications
East China University of Science and Technology
2014-2025
Chinese Academy of Sciences
2015-2024
Institute of Oceanology
2015-2024
Sichuan University
2018-2024
Sichuan Agricultural University
2010-2024
University of Chinese Academy of Sciences
2008-2024
Wuhan University of Science and Technology
2010-2024
Guangxi Medical University
2024
First Affiliated Hospital of GuangXi Medical University
2024
Qingdao National Laboratory for Marine Science and Technology
2018-2023
MicroRNAs (miRNAs) are endogenously encoded small noncoding RNAs, derived by processing of short RNA hairpins, that can inhibit the translation mRNAs bearing partially complementary target sequences. In contrast, interfering RNAs (siRNAs), which long double-stranded and often exogenous origin, degrade fully Here, we demonstrate an human miRNA is able to cleave mRNA sites, whereas exogenously supplied siRNA expression sequences without inducing detectable cleavage. These data suggest miRNAs...
Most eukaryotes encode a substantial number of small noncoding RNAs termed micro (miRNAs). Previously, we have demonstrated that miR-30, 22-nucleotide human miRNA, can be processed from longer transcript bearing the proposed miR-30 stem-loop precursor and translationally inhibit an mRNA-bearing artificial target sites. We also stem substituted with heterologous stem, which to yield novel miRNAs block expression endogenous mRNAs. Here, show second miR-21, effectively expressed when its forms...
Adaptive changes to oxygen availability are critical for cell survival and tissue homeostasis. Prolonged deprivation due reduced blood flow cardiac or peripheral tissues can lead myocardial infarction vascular disease, respectively. Mammalian cells respond hypoxia by modulating oxygen-sensing transducers that stabilize the transcription factor hypoxia-inducible 1α (HIF-1α), which transactivates genes governing angiogenesis metabolic pathways. Oxygen-dependent in HIF-1α levels regulated...
Breast cancer (BC) is the most common and top cause of female mortality worldwide. The prognosis for patients with breast liver metastasis (BCLM) remains poor. Emerging studies suggest that circular RNAs (circRNAs) are associated progression BC. Exploration circRNAs presents a promising avenue identifying metastasis-targeting agents improving BCLM. Microarray bioinformatic analyses were used to analyze differentially expressed between three pairs BCLM primary roles hsa_circ_0060467...
Drosha is a member of the ribonuclease (RNase) III family that selectively processes RNAs with prominent double-stranded features. plays key role in generation precursor microRNAs from primary microRNA (pri-miRNA) transcripts animal cells, yet how recognizes its RNA substrates remains incompletely understood. Previous studies have indicated that, within context larger pri-miRNA, an ∼80-nucleotide-long hairpin structure necessary for processing by Drosha. Here, performing <i>in vitro</i>...
Ago (Argonaute) proteins are essential effectors of RNA-mediated gene silencing. To explore potential regulatory mechanisms for proteins, we examined the phosphorylation human Ago2. We identified serine-387 as major Ago2 site in vivo. Phosphorylation at was significantly induced by treatment with sodium arsenite or anisomycin, and arsenite-induced inhibited a p38 MAPK (mitogen-activated protein kinase) inhibitor, but not inhibitors JNK (c-Jun N-terminal MEK [MAPK/ERK...
MicroRNAs (miRNAs) are a class of small ~22 nt noncoding (nc) RNAs that regulate gene expression post-transcriptionally by direct binding to target sites on mRNAs. They comprise more than 1,000 novel species in mammalian cells and exert their function modulating through several different mechanisms, including translational inhibition, and/or degradation Mitochondria maintain express own genome, which is distinct from the nuclear transcriptional apparatus. Thus, they provide potential site...
microRNAs are widely expressed, ∼22-nt-long regulatory RNAs. They first transcribed as much longer primary transcripts, which then undergo a series of processing steps to yield the single-stranded, mature microRNAs, although mechanisms incompletely understood. Here, we show that terminal loop region human microRNA transcripts is an important determinant biogenesis. Mutations restrain inhibit Drosha well Dicer precursor in vitro . The inhibition may result from lower enzyme turnover on mutant...
Abstract Intracellular viscosity is a crucial parameter that indicates the functioning of cells. In this work, we demonstrate utility TPE‐Cy, cell‐permeable dye with aggregation‐induced emission (AIE) property, in mapping inside live Owing to AIE characteristics, both fluorescence intensity and lifetime are increased along an increase viscosity. Fluorescence imaging cells stained TPE‐Cy reveals lipid droplets much shorter than from general cytoplasmic region. The loose packing lipids droplet...
The activation of H2O is a key step the COS hydrolysis, which may be tuned by oxygen vacancy defects in catalysts. Herein, we have introduced Cu into Co3O4 to regulate defect content In situ DRIFTS and XPS spectra reveal that are adsorbed activated vacancy. 10 at% doped sample (10CuCo3O4) exhibits optimal activity, 100% conversion at 70 °C. improved vacancies CuCo3O4 accelerate form active OH. binds with hydroxyl intermediate HSCO2−, then –OH on reacts HSCO2− HCO3−. Meanwhile, catalyst high...
In the context of preparation liquid fuels derived from biomass with zero CO2 footprint, fatty acids appear as suitable feedstocks. However, reduction carboxylic acid groups usually requires harsh reaction conditions and development efficient solid catalysts. Herein, bimetallic Ni–Mo nitrides have been obtained by a procedure not involving nitridation corresponding oxide, but reductive decomposition tris(ethylenediamine)nickel molybdate at temperatures between 550 700 °C. Successful...
While numerous microRNAs (miRNAs) have been reported to alter their expression levels in human lung cancer tissues compared with normal tissues, the function of these miRNAs and contribution long process development remains largely unknown. We applied a tobacco-specific carcinogen-induced model investigate involvement early development, which could also provide information on potential, biomarkers cancers. Male F344 rats were first chronically treated...
Dicer cleaves double-stranded RNAs (dsRNAs) or precursor microRNAs (pre-miRNAs) to yield ≈ 22-nt RNA duplexes. The pre-miRNA structure requirement for human activity is incompletely understood. By large-scale in vitro dicing assays and mutagenesis studies, we showed that most, although not all, of the 161 tested pre-miRNAs efficiently. stable association with Dicer, as examined by gel shift assays, appears important but sufficient cleavage. Human tolerates remarkable structural variation its...
MicroRNAs (miRNAs) are small, non-coding RNAs capable of postranscriptionally regulating gene expression. Accurate expression profiling is crucial for understanding the biological roles miRNAs, and exploring them as biomarkers diseases.A novel, highly sensitive, reliable miRNA quantification approach,termed S-Poly(T) assay, designed. In this miRNAs subjected to polyadenylation reverse transcription with a primer that contains universal primer, Taqman probe, an oligo(dT)(11) sequence six...
Hypoxia-driven changes in the tumor microenvironment facilitate cancer metastasis. In present study, we investigated regulatory cross talk between endocytic pathway, hypoxia, and Dynamin 2 (DNM2), a GTPase, is critical mediator of endocytosis. Hypoxia decreased levels DNM2. DNM2 promoter has multiple hypoxia-inducible factor (HIF)-binding sites genetic deletion them relieved hypoxia-induced transcriptional suppression. Interestingly, reciprocally regulated HIF. Inhibition GTPase activity...
CRISPR-Cas9 system has been widely used for efficient genome editing. Although the structures of Cas9 protein in complex with single-guided RNA (sgRNA) and target DNA have resolved, molecular details about formation endonuclease R-loop structure remain elusive. Here we examine cleavage activities Streptococcus pyogenes (SpyCas9) its mutants using various sequences study conformational dynamics during binding single-molecule fluorescence energy transfer (smFRET) technique. Our results show...
Abstract There is growing evidence that microRNAs are implicated in pulmonary arterial hypertension (PAH), but underlying mechanisms remain elusive. Here, we identified miR-223 was significantly downregulated chronically hypoxic mouse and rat lungs, as well artery smooth muscle cells (PASMC) exposed to hypoxia. Knockdown of increased PASMC proliferation. In contrast, overexpression abrogated cell proliferation, migration stress fiber formation. Administering agomir vivo antagonized...
A wide array of wildlife species, including many animals, are used in traditional medicines across medicinal systems, Traditional Chinese Medicine (TCM). Due to over-exploitation and habitat loss, the populations animals commonly TCM have declined unable meet market demand. number measures been taken try reduce impact that this large growing for may on wild animal species. Consumer preferences behavior known play an important role consumption protection medicine, thus likely be factor...