A5055682800- Antibiotic Resistance in Bacteria
- Enterobacteriaceae and Cronobacter Research
- Bacterial Identification and Susceptibility Testing
- Infections and bacterial resistance
- Vibrio bacteria research studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial biofilms and quorum sensing
- Pneumonia and Respiratory Infections
- Tuberculosis Research and Epidemiology
- Antimicrobial Resistance in Staphylococcus
- Antibiotic Use and Resistance
- Burkholderia infections and melioidosis
- Pancreatitis Pathology and Treatment
- Chromium effects and bioremediation
- Urinary Tract Infections Management
- Clostridium difficile and Clostridium perfringens research
- Streptococcal Infections and Treatments
- Blood groups and transfusion
- Neonatal and Maternal Infections
- Brucella: diagnosis, epidemiology, treatment
- Plant Pathogenic Bacteria Studies
- Prion Diseases and Protein Misfolding
- Central Venous Catheters and Hemodialysis
- Berberine and alkaloids research
AHEPA University Hospital
2016-2020
Aristotle University of Thessaloniki
2016-2020
General Hospital of Serres
2008-2019
National and Kapodistrian University of Athens
2008-2017
University Hospital of Alexandroupolis
2015
Polymères, Biopolymères, Surfaces
2013
Normandie Université
2013
Centre National de la Recherche Scientifique
2013
General Hospital of Nikea
2010
Tzaneion General Hospital
2009
The increasing frequency of class A KPC enzymes and B metallo-β-lactamases (MBLs) among Enterobacteriaceae as well their possible co-production makes early differentiation urgent. simple phenotypic algorithm employing three combined-disc tests consisting meropenem alone with phenylboronic acid (PBA), EDTA, or both PBA EDTA was designed for the MBL enzymes. Augmentation zone inhibition by ≥5 mm considered a positive test result. total 141 genotypically confirmed carbapenemase-positive...
The worldwide increase in the occurrence and dissemination of KPC beta-lactamases among gram-negative pathogens makes critical early detection these enzymes. Boronic acid disk tests using different antibiotic substrates were evaluated for KPC-possessing Klebsiella pneumoniae isolates. A total 57 genotypically confirmed K. isolates with varying carbapenem MICs examined. To measure specificity tests, 106 non-KPC-possessing (89 17 Escherichia coli isolates) randomly selected those exhibiting...
The worldwide dissemination of Enterobacteriaceae producing AmpC β-lactamases and carbapenemases makes difficult the phenotypic detection extended-spectrum (ESBLs), as they may be masked by these additional enzymes. A modification CLSI ESBL confirmatory test was developed evaluated in a comparative study for its ability to successfully detect ESBLs among various (Klebsiella pneumoniae carbapenemase [KPC], VIM, NDM, OXA-48) plasmidic or derepressed AmpCs. modified performed with cefotaxime...
Two colistin-susceptible/colistin-resistant (Col(s)/Col(r)) pairs of Acinetobacter baumannii strains assigned to international clone 2, which is prevalent worldwide, were sequentially recovered from two patients after prolonged colistin administration. Compared with the respective Col(s) isolates (Ab248 and Ab299, both having a MIC 0.5 μg/ml), Col(r) (Ab249 Ab347, MICs 128 32 μg/ml, respectively) significantly overexpressed pmrCAB genes, had single-amino-acid shifts in PmrB protein,...
To investigate the first KPC carbapenemase-producing Klebsiella pneumoniae isolate from a Greek hospital, including phenotypic methods to aid recognition of this resistance type. A carbapenem-resistant clinical K. was recovered hospitalized patient. Detailed susceptibility testing carried out by agar dilution method. The screened and genotypic assays for presence various β-lactamases. Boronic acid disc tests were performed show ability these detect production enzymes. potential conjugal...
We evaluated boronic acid (BA)-based methods for their ability to detect extended-spectrum beta-lactamases (ESBLs) among clinical isolates of KPC-producing members the Enterobacteriaceae family. A total 155 Klebsiella pneumoniae (n = 141), Escherichia coli 6), Enterobacter aerogenes and oxytoca 2) genotypically confirmed be KPC producers were analyzed. As many as 118 harbored ESBLs (103 SHV-type ESBLs, 13 CTX-M-type 2 both SHV- ESBLs); remaining 37 negative ESBL production. The CLSI...
To report the identification of metallo-beta-lactamase (MBL) variant VIM-19 in a Klebsiella pneumoniae clinical strain co-producing KPC-2 carbapenemase, CMY-2 cephalosporinase and CTX-M-15 extended-spectrum beta-lactamase.MICs were determined by agar dilution. Phenotypic tests performed to detect carbapenemase production. PCR nucleotide sequencing used for bla gene types mapping integron carrying MBL gene. The location KPC alleles was investigated mating experiments, plasmid analysis...
Although numerous studies have documented outbreaks of carbapenem-resistant Klebsiella pneumoniae (CRKP) possessing various carbapenemases, reports on due to CRKP extended-spectrum β-lactamases (ESBLs) and/or AmpCs with porin lesions been limited. Here, we describe an outbreak caused by ertapenem-resistant, CTX-M-15-producing clonal K. strain expressing OmpK36 variant. From May 2012 November 2012, 37 ertapenem-resistant isolates phenotypically negative for carbapenemase production were...
Meropenem heteroresistance was investigated in six apparently meropenem-susceptible, Klebsiella pneumoniae carbapenemase (KPC)-producing K. (KPC-KP) clinical isolates, compared with that carbapenemase-negative, meropenem-susceptible controls. In population analyses, the KPC-KP isolates grew at meropenem concentrations of 64 to 256 microg/ml. Heteroresistant colonies had significantly elevated expression bla(KPC) gene native populations but did not retain when subcultured drug-free media....
Carbapenemase-producing Enterobacteriaceae (CPE) are rapidly spreading worldwide. Early detection of fecal CPE carriers is essential for effective infection control. Here, we evaluated the performance a meropenem combined disk test (CDT) differentiating isolates directly from rectal swabs. The screening method was applied 189 swabs hospitalized patients at high risk carriage. Swabs were suspended in 1 ml saline and cultured confluent growth onto MacConkey agar plate with (MER) alone, MER...
The current phenotypic methods for detecting carbapenemase-producing Enterobacteriaceae (CPE) allow differentiation between class A and B carbapenemases, but they cannot confirm in a single test D OXA-48 carbapenemase producers. In this study, we evaluated new test, the disk which is based on an imipenem two blank disks adjacent to disk, loaded with tested strain impregnated EDTA plus phenyl boronic acid (PBA), respectively. evaluation of was performed 81 genotypically confirmed...
The recent emergence of carbapenemase-producing Enterobacteriaceae strains represents a major threat for hospitalized patients. We document the dissemination and control Klebsiella pneumoniae clones in Greek hospital. During 3-year study period (January 2009 to December 2011), K. were isolated from clinical samples 73 individual Phenotyping molecular testing confirmed that 52 patients infected with carbapenemase 2 (KPC-2) producers, 12 VIM-1 remaining 9 isolates producing both KPC-2 enzymes....
Abstract Objectives NDM-producing Enterobacteriaceae clinical isolates remain uncommon in the European region. We describe emergence and broad dissemination of one successful NDM-1-producing Klebsiella pneumoniae clone Greek hospitals. Methods During a 4 year survey (January 2013–December 2016), 480 single-patient carbapenem non-susceptible K. isolates, phenotypically MBL positive, were consecutively recovered eight hospitals from different locations subjected to further investigation....
During a 3-year period (May 2005 to April 2008), series of 45 outpatients presented with community-onset urinary tract infections due carbapenem-resistant Pseudomonas aeruginosa isolates. Forty them had history previous hospitalization or exposure healthcare facilities, while the remaining five not been previously admitted our facilities elsewhere within preceding 12 months. In 18 outpatients, organisms caused recurrent infections, in three were also implicated bacteremic episodes. All...
The accurate phenotypic detection of Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae is an increasing necessity worldwide. We evaluated the performance boronic acid combined-disk tests using as substrate imipenem or meropenem and inhibitor KPC production 300 μg aminophenylboronic (APBA), 600 APBA, 400 phenylboronic (PBA). Tests were considered positive when increase in growth-inhibitory zone around a carbapenem disk with was 5 mm greater diameter containing alone....
ABSTRACT The aim of the present study was to investigate epidemiological link multidrug-resistant Klebsiella oxytoca isolates causing community-onset infections among patients attending our outpatient department and underlying resistance mechanisms. were tested by agar dilution MICs, phenotypic carbapenemase testing, enterobacterial repetitive intergenic consensus-PCR, pulsed-field gel electrophoresis (PFGE). PCR assays nucleotide sequencing employed for identification bla gene types mapping...
Abstract Objectives Dependence on linezolid was recently described as significant growth acceleration of linezolid-resistant Staphylococcus epidermidis (LRSE) isolates upon exposure. We investigated the possible contribution dependence to LRSE dissemination in Greece. Methods Linezolid resistance rates were estimated six tertiary hospitals located throughout Greece between 2011 and 2013. Sixty-three randomly selected recovered these during this period studied. Growth curve analysis conducted...