A5006129196- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Research in Cotton Cultivation
- Reproductive Biology and Fertility
- Immunotherapy and Immune Responses
- Advanced Cellulose Research Studies
- Lipoproteins and Cardiovascular Health
- Electrospun Nanofibers in Biomedical Applications
- Nanoplatforms for cancer theranostics
- Plant Molecular Biology Research
- Bladder and Urothelial Cancer Treatments
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- Coronary Interventions and Diagnostics
- Renal cell carcinoma treatment
- Plant Gene Expression Analysis
- Liver physiology and pathology
- Acute Myocardial Infarction Research
- Antiplatelet Therapy and Cardiovascular Diseases
- Advanced Materials and Mechanics
- Endometrial and Cervical Cancer Treatments
- Micro and Nano Robotics
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- AI in cancer detection
- Renal and Vascular Pathologies
Shanxi Academy of Medical Sciences
2025
Shanxi Medical University
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2023-2025
Central China Normal University
2022-2025
Southern University of Science and Technology
2020-2024
Fu Wai Hospital
2024
University of Warwick
2022-2024
Peking Union Medical College Hospital
2023
Peking University
2023
First Affiliated Hospital Zhejiang University
2023
Spheroids are a powerful tool for basic research and to reduce or replace in vivo (animal) studies but not routinely banked nor shared. Here, we report the successful cryopreservation of hepatocyte spheroids using macromolecular (polyampholyte) cryoprotectants supplemented into dimethyl sulfoxide (DMSO) solutions. We demonstrate that polyampholyte significantly increases post-thaw recovery, minimizes membrane damage related cryo-injury, remains extracellular space making it simple remove...
The aim of this study was to evaluate and compare the usefulness C-reactive protein (CRP)-triglyceride glucose (TyG) index (CTI) other insulin resistance (IR) or inflammatory indexes for predicting recurrent cardiovascular events in percutaneous coronary intervention (PCI)-treated patients. In addition, mediating effects systemic inflammation, represented by high-sensitive CRP (hs-CRP), on TyG index-associated adverse across different subgroups were also evaluated. formula calculating CTI...
3D cell assemblies such as spheroids reproduce the in vivo state more accurately than traditional 2D monolayers and are emerging tools to reduce or replace animal testing. Current cryopreservation methods not optimized for complex models, hence they easily banked widely used models. Here we use soluble ice nucleating polysaccharides nucleate extracellular dramatically improve spheroid outcomes. This protects cells beyond using DMSO alone, with major advantage that nucleators function...
For prevention of the coronavirus disease 2019 caused by novel SARS-CoV-2, an effective vaccine is critical. Herein, several potential peptide epitopes from spike protein SARS-CoV-2 have been synthesized and covalently linked with cross-reactive material (CRM197). Immunization mice resulting conjugates induced high titers IgG antibodies against protein. Importantly, post-immune sera effectively neutralized pseudovirus, suggesting identified are protective, these promising leads for...
Cotton provides the most abundant natural fiber for textile industry. The mature cotton largely consists of secondary cell walls with highest proportion cellulose and a small amount hemicellulose lignin. To dissect roles hemicellulosic polysaccharides during development, four IRREGULAR XYLEM 15 (IRX15) genes, GhIRX15-1/-2/-3/-4, were functionally characterized in cotton. These genes encode DUF579 domain-containing proteins, which are homologs AtIRX15 involved xylan biosynthesis. GhIRX15...
A subfamily of transcription factors known as HD-ZIP III plays distinct roles in the secondary cell wall biosynthesis, which could be attributed to quality cotton fiber and adaptation drought stress. In this study, 18 genes were identified genome wide from upland ( Gossypium hirsutum ). These are distributed on 14 different chromosomes, all them have undergone segmental duplications. Numerous cis -elements promoter regions, related phytohormone responses abiotic stresses. Expression...
SUMMARY Cotton fibers, essentially cellulosic secondary cell walls (SCWs) when mature, are the most important raw material for natural textiles. SCW cellulose biosynthesis determines fiber thickness and industrially quality parameters, such as strength length. However, transcriptional regulatory networks controlling formation remain incomplete. Here, we identify eight NAC domain proteins (GhNACs) that involved in synthesis. These GhNACs can form pairwise heterodimers may act dimers, or...
The aim of this study was to investigate lymphocyte subsets, especially natural killer (NK) cells, in patients with systemic sclerosis (SSc) and evaluate the diagnostic value NK cells secondary pulmonary arterial hypertension (PAH). A total 115 SSc 100 age- sex-matched health controls (HCs) were enrolled study. Flow cytometry employed quantify while association between disease activity as well PAH investigated further elucidate its potential. absolute count (CD3-CD56+) significantly...
Abstract Particulate embolic agents with calibrated sizes, which employ interventional procedures to achieve endovascular embolization, have recently attracted tremendous interest in therapeutic embolotherapies for a wide plethora of diseases. However, the particulate shape effect, may play critical role embolization performances, has been rarely investigated. Here, polyvinyl alcohol (PVA)‐based shape‐anisotropic microembolics are developed using facile droplet‐based microfluidic fabrication...
Spheroids mimic 3-D tissue niches better than standard cell cultures. Cryopreserving spheroids, however, remains challenging as conventional cryoprotectants do not mitigate all damage mechanisms. Here chemically-programmed extracellular ice nucleation is used to prevent supercooling, alongside proline pre-conditioning, which are found synergystically improve post-thaw recovery of spheroids. This validates the need identify compounds and materials address both biochemical biophysical pathways...
Cell-based models, such as organ-on-chips, can replace and inform in vivo (animal) studies for drug discovery, toxicology, biomedical science, but most cannot be banked "ready to use" they do not survive conventional cryopreservation with DMSO alone. Here, we demonstrate how macromolecular ice nucleators enable the successful of epithelial intestinal models supported upon interface transwells, allowing recovery function just 7 days post-thaw directly from freezer, compared 21 suspension...
Atherogenic index of plasma (AIP) has been recommended as a marker atherogenicity. The impact AIP on plaque characteristics is not fully understood. study investigates the relationship between and coronary features in patients with acute syndrome (ACS). From January 2016 to June 2017 pre-intervention optical coherence tomography (OCT) was performed 522 ACS patients. defined base 10 logarithm ratio concentrations triglyceride high-density lipoprotein cholesterol. Patients were divided into...
Cotton provides abundant natural fibres for the textile industry. fibre development can be divided into five stages: initiation, elongation, transition, secondary cell wall (SCW) thickening and maturation. Fibre initiation elongation have been extensively studied, but transition stage remains less investigated. Although cellulose accounts >90% of mature fibres, non-cellulosic polysaccharides also contribute to development. Little is known about roles these carbohydrates during this process....
Triple negative breast cancer (TNBC) is the most aggressive in women, and despite improved treatments, it remains a major cause of morbidity mortality. We others have demonstrated that different hybrid compounds targeting PARP/MAPK or other pathways to inhibit progression may lead promising therapeutic results. introduced fluorine alter physical properties compounds. TSC-3C was one generated Upon treatment with TSC-3C, MDA-MB-231 cell proliferation, invasion, migration were inhibited....
Transarterial chemoembolization (TACE) has emerged as the mainstay treatment for patients suffering from unresectable intermediate hepatocellular carcinoma and also holds potential to treat other types of hypervascular cancers such renal cell carcinoma. However, an in vitro model evaluating both embolic performance drug-release kinetics TACE agents is still lacking since current models greatly simplified vivo vascular systems well extracellular matrices (ECM) organs. Here, we developed a...
Cancer is a multifaceted global health problem that requires continuous action to develop next-generation cancer theranostics. Inspired by the emerging use of indocyanine green (ICG), only clinically approved near-infrared (NIR) dye for phototherapy, here we synthesized two ICG conjugate theranostics coupling sialic acid (Sia) through C2 and C9 positions Sia, respectively, referred as Sia-C2-ICG Sia-C9-ICG. Encouragingly, Sia-C2/C9-ICGs show superior
Developing a three-dimensional (3D) in vitro tumor model with vasculature systems suitable for testing endovascular interventional therapies remains challenge. Here we develop an orthotopic liver spheroid that captures the organ-level complexity of and extracellular matrix to evaluate transcatheter arterial chemoembolization (TACE) treatment. The spheroids are derived by seeding HepG2 cell colonies controlled size location surrounding portal triads decellularized rat treated clinically...
Spheroids and other 3D cellular models more accurately recapitulate physiological responses when compared to 2D represent potential alternatives animal testing. The cryopreservation of spheroids remains challenging, limiting their wider use. Standard DMSO-only results in supercooling low subzero temperatures, reducing viability, shedding surface cells, perforating spheroid interiors. Here, cocultured with differentially labeled outer cell layers allow spatial evaluation the protective effect...