A5074755592- Turfgrass Adaptation and Management
- Reproductive Biology and Fertility
- 3D Printing in Biomedical Research
- Insect and Arachnid Ecology and Behavior
- Hemoglobin structure and function
- Healthcare Systems and Challenges
- Magnetic and Electromagnetic Effects
- Clinical Nutrition and Gastroenterology
- Plant and animal studies
- Origins and Evolution of Life
- Microbial Inactivation Methods
- Laser-Ablation Synthesis of Nanoparticles
- Physiological and biochemical adaptations
- Planarian Biology and Electrostimulation
- Tissue Engineering and Regenerative Medicine
- Spectroscopy and Quantum Chemical Studies
- Atmospheric chemistry and aerosols
- Microfluidic and Bio-sensing Technologies
- nanoparticles nucleation surface interactions
- Laser-induced spectroscopy and plasma
- Dendrimers and Hyperbranched Polymers
- Calcium Carbonate Crystallization and Inhibition
- Crystallization and Solubility Studies
- Surface Modification and Superhydrophobicity
- Botany and Plant Ecology Studies
University of Warwick
2021-2024
Royal Botanic Gardens, Kew
2024
University of Edinburgh
2019
3D cell assemblies such as spheroids reproduce the in vivo state more accurately than traditional 2D monolayers and are emerging tools to reduce or replace animal testing. Current cryopreservation methods not optimized for complex models, hence they easily banked widely used models. Here we use soluble ice nucleating polysaccharides nucleate extracellular dramatically improve spheroid outcomes. This protects cells beyond using DMSO alone, with major advantage that nucleators function...
Cryopreservation of biological material is vital for existing and emerging biomedical biotechnological research related applications, but there remain significant challenges. cells in sub-milliliter volumes difficult because they tend to deeply supercool, favoring lethal intracellular ice formation. Some tree pollens are known produce polysaccharides capable nucleating at warm sub-zero temperatures. Here we demonstrated that aqueous extractions from European hornbeam pollen (pollen washing...
Abstract. Ice-nucleating macromolecules (INMs) produced by plant pollen can nucleate ice at warm temperatures and may play an important role in weather- climate-relevant cloud glaciation. INMs have also proved useful for mammalian cell tissue model cryopreservation. The high nucleation (IN) activity of some indicates underlying biological function, either freezing tolerance or bioprecipitation-mediated dispersal. Here, using the largest study to date, we show that phylogenetic proximity,...
Ice-nucleating particle (INP) concentration in the atmosphere over Southern Ocean represents a significant source of uncertainty representation clouds global climate models. A better understanding sources, properties and abundance INPs this region is essential to reduce (Murray, 2021). There increasing evidence suggest that ice nucleators are produced ubiquitously by land plants. Yet, molecular basis evolutionary origins these remain obscure. Adapted for cold- drought-tolerance, mosses...
Spheroids mimic 3-D tissue niches better than standard cell cultures. Cryopreserving spheroids, however, remains challenging as conventional cryoprotectants do not mitigate all damage mechanisms. Here chemically-programmed extracellular ice nucleation is used to prevent supercooling, alongside proline pre-conditioning, which are found synergystically improve post-thaw recovery of spheroids. This validates the need identify compounds and materials address both biochemical biophysical pathways...
Cell-based models, such as organ-on-chips, can replace and inform in vivo (animal) studies for drug discovery, toxicology, biomedical science, but most cannot be banked "ready to use" they do not survive conventional cryopreservation with DMSO alone. Here, we demonstrate how macromolecular ice nucleators enable the successful of epithelial intestinal models supported upon interface transwells, allowing recovery function just 7 days post-thaw directly from freezer, compared 21 suspension...
We report on a study of laser-induced nucleation (LIN) sodium chlorate in supersaturated aqueous solutions using focused nanosecond laser pulses at high energy densities (420 kJ cm–2). On irradiation with single pulse, optical breakdown was observed the form luminous plasma, and numerous microbubbles were produced. basis observations, we estimate threshold for to be 70 J cm–2. Remarkably, even densities, produced average only one or two crystals. The mean number crystals obtained 1.5 (532...
Abstract. Ice nucleating molecules (INMs) produced by plant pollen can nucleate ice at warm temperatures and may play an important role in weather climate relevant cloud glaciation. INMs have also proved useful for mammalian cell tissue model cryopreservation. The high nucleation (IN) activity of some indicates underlying biological function, either freezing tolerance or bioprecipitation mediated dispersal. Here, using the largest study to date, we show that phylogenetic proximity,...
Ice binding proteins (IBP) have evolved to limit the growth of ice but also promote formation by ice-nucleating (INPs). IBPs, which modulate these seemingly distinct processes, often high sequence similarities, and molecular size/assembly is hypothesized be a crucial determinant. There are only few synthetic materials that reproduce INP function, rational design nucleators has not been achieved due outstanding questions about mechanisms binding. Poly(vinyl alcohol) (PVA) water-soluble...
Soluble molecules released from plant pollen can nucleate ice supercooled water and are an enigmatic source of atmospherically relevant biological nucleators. Recently, it has been highlighted that nucleating particles may possess greater potential to impact cloud glaciation than previously considered, as fragments generated by bursting under atmospheric conditions could act carriers molecules, with significantly longer residence times whole grains1,2. Previous studies have indicated a range...