A5028352862- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Mycobacterium research and diagnosis
- Aquaculture disease management and microbiota
- Bacterial Genetics and Biotechnology
- Infectious Diseases and Mycology
- Ginseng Biological Effects and Applications
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Animal Virus Infections Studies
- Probiotics and Fermented Foods
- Antibiotic Resistance in Bacteria
- Pharmacological Effects of Natural Compounds
- Viral gastroenteritis research and epidemiology
- Pneumonia and Respiratory Infections
- Enzyme Production and Characterization
- Salmonella and Campylobacter epidemiology
- Biochemical and Structural Characterization
- Veterinary medicine and infectious diseases
- Chromium effects and bioremediation
- Glycosylation and Glycoproteins Research
- Respiratory viral infections research
- Biofuel production and bioconversion
- Cancer Research and Treatments
- Enzyme Structure and Function
University of Veterinary Medicine Hannover, Foundation
2014-2025
University of Veterinary Medicine Vienna
2005-2025
Forschungszentrum Jülich
1993-2006
Leibniz University Hannover
2000-2005
Infectious Diseases Institute
2005
The probiotic bacterial strain Enterococcus faecium SF68 has been shown to alleviate symptoms of intestinal inflammation in human clinical trials and animal feed supplementation studies. To identify factors involved immunomodulatory effects on host cells, E. other commensal isolates were screened using epithelial cell lines harboring reporter fusions for NF-κB JNK(AP-1) activation determine the responses innate immune signaling pathways when challenged with protein components. Cell-free,...
ABSTRACT Zoonotic infections caused by Salmonella enterica serovar Typhimurium pose a constant threat to consumer health, with the pig being particularly major source of multidrug-resistant isolates. Vaccination, as promising approach reduce colonization and shedding, has been scarcely used, it interferes current control programs relying on serology means herd classification. In order overcome this problem, we set out develop negative-marker vaccine allowing differentiation infected from...
ABSTRACT Group 2 capsule polymers represent crucial virulence factors of Gram-negative pathogenic bacteria. They are synthesized by enzymes called polymerases. In this report, we describe a new family polymerases that combine glycosyltransferase and hexose- polyol-phosphate transferase activity to generate complex poly(oligosaccharide phosphate) poly(glycosylpolyol polymers, the latter which display similarity wall teichoic acid (WTA), cell component Gram-positive Using modeling...
The enrichment of open reading frames (ORFs) from large gene libraries and the presentation corresponding polypeptides on filamentous phage M13 (phage display) is frequently used to identify binding partners unknown ORFs. In particular display a valuable tool for identification pathogen-related antigens first step development new diagnostics therapeutics. Here, we introduce significant improvement phage-based ORF by using Hyperphage, helperphage with truncated gIII. methods allow both ORFs...
Mycoplasma mycoides subsp. capri (Mmc) and (Mmm) are important ruminant pathogens worldwide causing diseases such as pleuropneumonia, mastitis septicaemia. They express galactofuranose residues on their surface, but role in pathogenesis has not yet been determined. The M. genomes contain up to several copies of the glf gene, which encodes an enzyme catalysing last step synthesis galactofuranose. We generated a deletion gene strain Mmc using genome transplantation tandem repeat endonuclease...
The Gram-negative rod Actinobacillus pleuropneumoniae is a facultative anaerobic pathogen of the porcine respiratory tract, and HlyX, A. homologue fumarate nitrate reduction regulator (FNR), has been shown to be important for persistence. An hlyX deletion mutant decreased generation time but highly prolonged survival in comparison its wild type parent strain when grown anaerobically glucose-supplemented medium. Applying combination proteomic transcriptomic approaches as well silico analyses,...
Heterologous protein secretion was studied in the gram-positive bacteria Bacillus subtilis and Staphylococcus carnosus by using Escherichia coli outer membrane OmpA as a model protein. The found to be translocated across plasma of both microorganisms. However, majority similarly degraded B. S. despite fact that latter organism does not secrete soluble exoproteases into culture medium. finding purified OmpA, which added externally medium growing cells, remained intact indicates newly...
In order to investigate the role of ferric uptake regulator Fur in porcine lung pathogen Actinobacillus pleuropneumoniae, we constructed an isogenic in-frame deletion mutant, A. pleuropneumoniae Deltafur. This mutant showed constitutive expression transferrin-binding proteins, growth deficiencies vitro, and reduced virulence aerosol infection model.
ABSTRACT Vaccination against Actinobacillus pleuropneumoniae is hampered by the lack of vaccines inducing reliable cross-serotype protection. In contrast, pigs surviving natural infection are at least partially protected from clinical symptoms upon reinfection with any serotype. Thus, we set out to construct an attenuated A. live vaccine allowing differentiation vaccinated infected animals (the DIVA concept) successively deleting virulence-associated genes. Based on serotype 2 prototype...
Knowledge on the proteome level about adaptation of pathogenic mycobacteria to environment in their natural hosts is limited. Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne's disease, a chronic and incurable granulomatous enteritis ruminants, has been suggested be putative aetiological agent Crohn's disease humans. Using comprehensive LC-MS-MS 2D difference gel electrophoresis (DIGE) approach, we compared protein profiles clinical strains MAP prepared from gastrointestinal...
The ferric uptake regulator A (FurA) is known to be involved in iron homeostasis and stress response many bacteria. In mycobacteria the precise role of FurA still unclear. presented study, we addressed functional ruminant pathogen Mycobacterium avium ssp. paratuberculosis (MAP) by construction a furA deletion strain (MAPΔfurA). RNA deep sequencing revealed that regulon consists repressed activated genes associated or intracellular survival. Not single gene related metal was affected...
Zinc homeostasis is crucial for bacterial cells, since imbalances affect viability. However, in mycobacteria, knowledge of zinc metabolism incomplete. Mycobacterium smegmatis (MSMEG) an environmental, nonpathogenic that widely used as a model organism to study mycobacterial and pathogenicity. How MSMEG maintains largely unknown. SmtB Zur are important regulators metabolism. In these encoded by operon, whereas other species, on separate loci. Here, we show the smtB-zur operon consistently...
Summary The translocation of secretory proteins derived from a Gram‐positive (Staphylococcus hyicus prolipase) or Gram‐negative (Escherichia coli pre‐OmpA protein) bacterium across the cytoplasmic membrane was studied in E. and Bacillus subtilis. both microorganisms, prolipase found to be secreted plasma when either pre‐prolipase signal peptide (38 amino acids length) (21 used. Expression gene encoding authentic protein B. subtilis resulted mature OmpA membrane. Processing precursor required...
Maintenance of metal homeostasis is crucial in bacterial pathogenicity as starvation the most important mechanism nutritional immunity strategy host cells. Thus, pathogenic bacteria have evolved sensitive scavenging systems to overcome this particular defence mechanism. The ruminant pathogen Mycobacterium avium ssp. paratuberculosis (MAP) displays a unique gut tropism and causes chronic progressive intestinal inflammation. MAP possesses eight conserved lineage specific large sequence...