A5007437959- Antimicrobial Resistance in Staphylococcus
- Salmonella and Campylobacter epidemiology
- Bacterial biofilms and quorum sensing
- Viral gastroenteritis research and epidemiology
- Probiotics and Fermented Foods
- Clostridium difficile and Clostridium perfringens research
- Gut microbiota and health
- Milk Quality and Mastitis in Dairy Cows
- Bacterial Identification and Susceptibility Testing
- Bacteriophages and microbial interactions
- Mycobacterium research and diagnosis
- Microbial Metabolism and Applications
- Cancer, Hypoxia, and Metabolism
- Molecular Biology Techniques and Applications
- Food Safety and Hygiene
- Escherichia coli research studies
- Aquaculture disease management and microbiota
- Listeria monocytogenes in Food Safety
- Microbial infections and disease research
- Microscopic Colitis
- Bacterial Genetics and Biotechnology
- Protein purification and stability
- Helicobacter pylori-related gastroenterology studies
- Complement system in diseases
- Immune Response and Inflammation
Federal Institute for Risk Assessment
2014-2025
Freie Universität Berlin
2011-2022
Abstract Background Campylobacter jejuni and coli are human intestinal pathogens of global importance. Zoonotic transmission from livestock animals or animal-derived food is the likely cause for most these infections. However, little known about their general host-specific mechanisms colonization, virulence pathogenicity factors. In certain hosts, species colonize persistently do not disease, while they acute disease in humans. Results Here, we investigate putative host-specificity using...
The probiotic bacterial strain Enterococcus faecium SF68 has been shown to alleviate symptoms of intestinal inflammation in human clinical trials and animal feed supplementation studies. To identify factors involved immunomodulatory effects on host cells, E. other commensal isolates were screened using epithelial cell lines harboring reporter fusions for NF-κB JNK(AP-1) activation determine the responses innate immune signaling pathways when challenged with protein components. Cell-free,...
Background: Methicillin-resistant Staphylococcus aureus (MRSA) has emerged as a major threat to public health. This study investigated the occurrence of MRSA in humans, chickens, chicken meat and environmental samples within poultry farms live bird markets southwestern Nigeria. Methods: were isolated using selective culture tested for antimicrobial susceptibility by broth microdilution. Selected isolates characterized whole genome sequencing (WGS). From WGS data, spa, dru, multilocus...
Methicillin-resistant Staphylococcus aureus (MRSA) have been found on German dairy farms and may be the cause of difficult-to-treat bovine mastitis. Considering one health approach, MRSA might transmitted from animals to humans raising risk for severe infections. On 17 with a history detection, strains were isolated quarter milk, bulk tank swab samples calves, heifers, pigs, environment. A selection 33 isolates was analyzed using whole-genome sequencing antimicrobial resistance testing. All...
Staphylococcal enterotoxins (SEs) are major contributors to foodborne intoxications. Reliable detection methods for SEs essential maintain food safety and protect public health. Since the heat-stable toxins also exert their toxic effect in absence of bacterium, reliance on DNA alone can be misleading: it does not allow determining which specific encoded by a given strain produced epidemiologically linked with outbreak. Commercially available diagnostic assays SE so far limited sensitivity...
Clostridioides difficile is one of the most frequent causes nosocomial infections in humans leading to (antibiotic-associated) diarrhea and severe pseudomembranous colitis. With an increasing frequency, C. (CDI) are also observed independently hospitalization age patients ambulant setting. One potential source so-called community-acquired CDI a zoonotic transmission based on direct contact with animals or consumption food. To estimate exposure via food, we screened 364 different retail fresh...
Mammaliicocci might play a major role in antimicrobial resistance (AMR) gene transmission between organisms of the family Staphylococcaceae, such as potentially pathogenic species Staphylococcus aureus. The interest this study was to analyze AMR profiles mammaliicocci from German dairy farms evaluate potential. In total, 65 isolates 17 with history MRSA detection were analyzed for genotypes and phenotypes using whole genome sequencing susceptibility testing against 19 antibiotics. various...
Abstract Staphylococcus aureus subsp. anaerobius (SAAN) causes abscesses in small ruminants, known as Morel’s Disease. This study describes the presence of SAAN for first time Germany and Austria examines phylogenetic relationship among these isolates previously described European Sudanese ones. A total 35 sheep 10 goat from 12 herds were available analysis. four Polish goats three Austrian different included. Genome comparisons analyses conducted using core genome multilocus sequence...
Staphylococcus aureus is an important opportunistic pathogen of both humans and animals. It can cause several diseases, including mastitis, as well food poisoning by production heat-stable enterotoxins in food. The aim this study was to determine the prevalence S. diversity strains circulating Zambian dairy value chain, which have not been studied detail before. Three provinces were covered (Lusaka, Southern, Western) almost 2000 samples along covering informal formal market sectors, taken...
The human diarrheal pathogens Campylobacter jejuni and coli interfere with host innate immune signaling by different means, their flagellins, FlaA FlaB, have a low intrinsic property to activate the receptor Toll-like 5 (TLR5). We investigated here hypothesis that unusual secreted, flagellin-like molecule FlaC present in C. jejuni, coli, other Campylobacterales might cells via TLR5 interact TLR5. shows striking sequence identity its D1 domains TLR5-activating flagellins of bacteria, such as...
has significant clinical importance as a leading cause of healthcare-associated infections, with symptoms ranging from mild diarrhoea to severe colitis, and possible life-threatening complications.
Abstract Non- aureus staphylococci are commonly found on dairy farms. Two rarely investigated species Staphylococcus ( S .) cohnii and . urealyticus Since multidrug-resistant S. known, they may serve as an antimicrobial resistance (AMR) gene reservoir for harmful staphylococcal species. In our study, nine six isolates from German farms were analyzed by whole-genome sequencing AMR testing. The harbored various genes aadD1 , str mecA dfrC/K tetK/L ermC lnuA fexA fusF fosB6 qacG/H ) exhibited...
Methicillin-resistant Staphylococcus aureus (MRSA) are a major threat to human and animal health, causing difficult-to-treat infections. The aim of our study was evaluate the intra-farm transmission livestock-associated (LA) MRSA sequence type (ST) 398 isolates on German dairy farms. A total 115 LA-MRSA ST398 originating from animals, humans environment six farms were analyzed by whole-genome sequencing core genome multilocus typing. Phylogenetic clusters high allelic similarity detected all...
Resistance of methicillin-resistant Staphylococcus aureus (MRSA) from food and livestock to last resort antibiotics such as linezolid is highly concerning, since treatment options for infections in humans might be diminished. Known mechanisms resistance include point mutations the 23S rRNA gene ribosomal proteins L3, L4 L22 well an acquisition cfr, optrA or poxtA gene. The objective our study was characterize antimicrobial (AMR) determinants phylogenetic relationships among...
Mannose-binding lectin (MBL) is a key molecule in innate immunity. MBL binds to carbohydrates on the surface of pathogens, initiating complement system via lectin-dependent pathway or facilitates opsonophagocytosis. In vivo studies using inbred chicken lines differing serum concentration indicate that affects Salmonella resistance; further are imperative conventional broiler chickens. this study 104 day-old chickens (offspring from cross between Cobb 500 male and female parent breeders) were...
Abstract Objective Methicillin-resistant Staphylococcus aureus (MRSA) have repeatedly been isolated from dairy herds. It was the purpose of this study to compare results 3 subsequent national scale cross-sectional investigations in herds Germany on prevalence MRSA bulk tank milk and characteristics isolates. Material Methods The were carried out 2010, 2014 2019, respectively. 25 ml using a double selective enrichment protocol. Samples distributed across country according regional cattle...
In recent years, methicillin-resistant Staphylococcus aureus (MRSA) has gained interest in veterinary medicine due to its zoonotic potential. Currently, little information is available on the genotypic and virulence characteristics of MRSA isolates detected Nigerian abattoirs. To better understand epidemiology associated with abattoir food chain environment Nigeria, a total 18 (humans: n = 5, slaughter animals: environment: 8), previously spa typed, were recovered characterized by cassette...
Intensive research regarding the impact of land use-related factors on prevalence and distribution antimicrobial-resistant Staphylococcaceae in game ungulate populations is necessary for adequately determining risks related to interactions between wild animals, domestic humans common geographic locations. This systematic approach analysis observations specific hunting districts Brandenburg, Germany, adds an innovative value strategy antimicrobial resistance which accordance with current...