A5013496925- Clostridium difficile and Clostridium perfringens research
- Nosocomial Infections in ICU
- Antimicrobial Resistance in Staphylococcus
- Viral gastroenteritis research and epidemiology
- Microscopic Colitis
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Family Support in Illness
- Helicobacter pylori-related gastroenterology studies
- Streptococcal Infections and Treatments
- Gut microbiota and health
- Sociology and Education Studies
- Social and Demographic Issues in Germany
- Hippo pathway signaling and YAP/TAZ
- Geriatric Care and Nursing Homes
- Psychiatric care and mental health services
- Bacterial biofilms and quorum sensing
- Antibiotic Use and Resistance
- Ubiquitin and proteasome pathways
- Infective Endocarditis Diagnosis and Management
- Family and Disability Support Research
- Healthcare innovation and challenges
- Psychology, Coaching, and Therapy
- Insect and Pesticide Research
- Social Policies and Healthcare Reform
Saarland University
2017-2025
Institute of Medical Microbiology and Hygiene
2016-2025
University of Regensburg
2022-2023
Furtwangen University
2022
Osnabrück University
2022
ZHAW Zurich University of Applied Sciences
2022
Ostschweizer Fachhochschule OST
2022
SRH Hochschule für Gesundheit Gera
2020
University of Teacher Education in Special Needs
2020
Hôpital d'instruction des Armées Percy
2018
Background: There are inconsistent data on the risk factors for Clostridium difficile infection (CDI) in literature. Aims: To use two C. case-control study groups to compare hospitalized patients with diarrhea across different countries. Methods: A multi-center group of CDI cases/controls were identified by standardized testing from seven countries prior EUropean, multi-center, prospective bi-annual point prevalence CLostridium Infection Diarrhea (EUCLID). second was a single center Germany...
We analyzed consecutive clinical cases of infections due to carbapenemase-producing gram-negative bacteria detected in war-wounded patients from Ukraine who were treated at one university medical center southwest Germany between June and December 2022. The isolates multiresistant subjected a thorough microbiological characterization whole genome sequencing (WGS). identified five Ukrainian developed with New Delhi metallo-β-lactamase 1-positive Klebsiella pneumoniae. Two also carried OXA-48...
Clostridioides difficile is the most important pathogen causing antimicrobial-associated diarrhea and has recently been recognized as a cause of community-associated C. infection (CA-CDI). This study aimed to characterize virulence factors, antimicrobial resistance (AMR), ribotype (RT) distribution genetic relationship isolates from diverse fecally contaminated environmental sources. were recovered different samples in Northern Germany. Antimicrobial susceptibility testing was determined by...
Flavonifractor plautii (formerly Eubacterium plautii) is an anaerobic gram positive rod shaped bacterium belonging to the family of Clostridiales, and a common member human gut microbiome. However, it very rarely isolated from clinical specimens, so data about its significance are scarce. Here we report bloodstream infection due F. following gangrenous cholecystitis in 69 year old man. After cholecystectomy empirical antimicrobial treatment with ceftriaxone metronidazole patient recovered....
Three German patients developed nosocomial pneumonia after cardiac surgery and had Burkholderia cepacia complex detected in respiratory specimens. Two died of septic multi-organ failure. Whole-genome sequencing genetically identical B. strains patient samples, from a batch octenidine mouthwash solution, which been used for nursing care, as well samples obtained the manufacturer during production. Contamination medical products manufacturing may lead to international outbreaks.
In light of the global antimicrobial-resistance crisis, there is an urgent need for novel bacterial targets and antibiotics with modes action. It has been shown that Pseudomonas aeruginosa elastase (LasB) Clostridium histolyticum (Hathewaya histolytica) collagenase (ColH) play a significant role in infection process thereby represent promising antivirulence targets. Here, we report N-aryl-3-mercaptosuccinimide inhibitors target both LasB ColH, displaying potent activities vitro high...
Intrahepatic cholangiocarcinoma (iCCA) is a highly aggressive primary liver tumor with increasing incidence worldwide, dismal prognosis, and few therapeutic options. Mounting evidence underlines the role of Hippo pathway in this disease; however, molecular mechanisms whereby cascade contributes to cholangiocarcinogenesis remain poorly defined.We established novel iCCA mouse models via hydrodynamic transfection an activated form transcriptional coactivator PDZ-binding motif (TAZ), downstream...
B-cell-depleting therapies are increasingly being used to treat autoimmune diseases. Although thousands of patients and have been treated with these agents, the data not yet strong enough identify rare side effects certainty. We report case a patient undergoing ocrelizumab therapy for relapsing multiple sclerosis who developed severe limbic syndrome. Autoimmune pathogenesis was initially suspected, worsening on immunosuppressive therapy. Later, after diagnosis an enterovirus infection,...
International travellers have the potential to transmit multidrug-resistant bacteria. However, role of hygiene measures during travel has yet be investigated. Travellers were asked about their use disinfectants and behaviour. Stool samples analysed for Gram-negative multi-resistant bacteria (MDRO). These data analysed, a new MDRO risk map was developed compared with from existing literature. Of 214 travellers, 6 (2.8%) tested positive an before 16 (7.5%) after trip, in known high-risk areas....
Clostridium (Clostridioides) difficile is the main cause for nosocomial diarrhoea in industrialised nations. Epidemiologic data on pathogen's occurrence other world regions are still scarce. In this context we characterized with phenotypic and molecular genetic methods C. isolates stemming from hospitalised patients Lebanon. From 129 stool samples of symptomatic at a tertiary care University hospital Lebanon, total 107 strains were cultivated underwent ribotyping, toxin gene detection...
Clostridioides difficile is a Gram positive spore-forming rod and mainly responsible for nosocomial diarrhea in developed nations. Molecular antimicrobial surveillance important monitoring the strain composition including genotypes of high epidemiological importance such as ribotype 027 (RT027) corresponding resistance patterns. 1535 isolates obtained from samples sent between 2014 2019 to German National Reference Center (NRC) diagnostic reasons (NRC set), 1143 Tertiary Care University...
Hypervirulent ribotypes (HVRTs) of Clostridioides difficile such as ribotype (RT) 027 are epidemiologically important. This study evaluated whether MALDI-TOF can distinguish between strains HVRTs and non-HVRTs commonly found in Europe. Obtained spectra clinical C. isolates (training set, 157 isolates) covering relevant Europe were used an input for different machine learning (ML) models. Another 83 a validation set. Direct comparison obtained from did not allow to discriminate these two...
Background Predictive machine learning models have made use of a variety scoring systems to identify clinical deterioration in ICU patients. However, most these scores include variables that are dependent on medical staff examining the patient. We present development real-time prediction model using digital and automatically generated for early detection patients at risk deterioration. Methods Routine monitoring data were used this analysis. with least 24 h vital sign recordings included....
Clostridioides difficile infections (CDI) present significant health risks and are among the most important nosocomial infections. Ribotype (RT) 027 poses a particular risk due to its proposed "hypervirulence". Traditionally, C. isolates characterized using PCR-ribotyping. More recently, whole genome sequence (WGS) analysis is increasingly used, which may provide higher discriminatory power. This study aimed assess distribution of different RTs in hospitals Berlin-Brandenburg area, analyse...
Nosocomial Clostridioides difficile infection (CDI) complicates up to 1% of all hospital admissions and is associated with considerable health burden. To determine the incidence outcomes nosocomial CDI at a major University Medical Center. Consecutive adult cases were prospectively identified. Stool samples collected for ribotyping antibiotic resistance testing. Patients followed eight weeks after discharge relapse. Over 2-year period, 215 patients developed (incidence 2:1000) 200 (mean age...
Clostridioides difficile, a Gram-positive spore-forming bacterium, is the leading cause of nosocomial diarrhea worldwide and therefore substantial burden to healthcare system. During past decade, hypervirulent PCR-ribotype (RT) 027 population emerged rapidly all over world, associated with both, increased severity mortality rates. It thus great importance identify epidemic strains such as RT027 fast possible. While commonly used diagnostic methods, e.g. multi locus sequence typing (MLST) or...
Clostridioides difficile is the major cause of infectious nosocomial diarrhoea in industrialized nations. Data on occurrence C. Africa, ribotype (RT) distribution, antimicrobial susceptibility patterns and potential zoonotic transmission are scarce.80 Zimbabwean isolates from different sources (chicken [n=30], soil [n=21] humans [n=29]) were investigated using ribotyping, toxin gene detection, resistance testing, multiple-locus variable-number tandem repeat analysis (MLVA), whole genome...
Here we describe a cluster of hospital-acquired Clostridium difficile infections (CDI) among 26 patients with osteoarticular infections. The aim the study was to define source C. and evaluate impact general infection control measures antibiotic stewardship on incidence CDI.Epidemiological analysis included typing strains possible patient transmission. Infection comprised strict isolation CDI patients, additional hand washings, intensified environmental cleaning sporicidal disinfection. In...
Abstract Background The unrestricted use of linezolid has been linked to the emergence linezolid-resistant Staphylococcus epidermidis (LRSE). We report effects combined antibiotic stewardship and infection control measures on spread LRSE in an intensive care unit (ICU). Methods Microbiological data were reviewed identify all detected clinical samples at ICU southwest Germany. Quantitative antibiotics with Gram-positive coverage obtained defined daily doses (DDD) per 100 patient-days (PD). In...