A5068544400- Myasthenia Gravis and Thymoma
- Cell Image Analysis Techniques
- Antifungal resistance and susceptibility
- Acute Ischemic Stroke Management
- Mitochondrial Function and Pathology
- Peripheral Neuropathies and Disorders
- Parkinson's Disease and Spinal Disorders
- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- S100 Proteins and Annexins
- Cancer, Hypoxia, and Metabolism
- Traumatic Brain Injury and Neurovascular Disturbances
- Animal testing and alternatives
- RNA Interference and Gene Delivery
- Anesthesia and Neurotoxicity Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Tracheal and airway disorders
- DNA and Nucleic Acid Chemistry
- Autophagy in Disease and Therapy
- Adipose Tissue and Metabolism
- Thyroid Disorders and Treatments
- Climate Change and Health Impacts
- Neurogenetic and Muscular Disorders Research
- Neurological Disorders and Treatments
- Cystic Fibrosis Research Advances
Charité - Universitätsmedizin Berlin
2016-2025
University of Oxford
2023-2025
Stroke Association
2025
Humboldt-Universität zu Berlin
2004-2024
Freie Universität Berlin
2020-2024
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2017-2021
Sunnybrook Health Science Centre
2015-2021
University of Toronto
2015-2021
University Hospital Heidelberg
2019
University Medical Center
2019
Myasthenic crisis (MC) and disease exacerbation in myasthenia gravis (MG) are associated with significant lethality continue to impose a high burden on affected patients. Therefore, we sought determine potential predictors for MC as well identify factors affecting outcome.
To determine demographic characteristics, clinical features, treatment regimens, and outcome of myasthenic crisis (MC) requiring mechanical ventilation (MV).Analysis patients who presented with MC between 2006 2015 in a German multicenter retrospective study.We identified 250 cases 12 participating centers. Median age at was 72 years. duration MV days. Prolonged (>15 days) depended on (p = 0.0001), late-onset myasthenia gravis (MG), high Myasthenia Gravis Foundation America Class before...
Dopamine is the predominant catecholamine in brain and functions as a neurotransmitter. also potent immune modulator. In this study, we have characterized expression of dopamine receptors on murine microglia. We found that cultured primary microglia express D1, D2, D3, D4, D5 receptors. specifically focused D2 receptor (D2R), major target antipsychotic drugs. Whereas D2Rs were strongly expressed striatal neurons vivo, did not detect any D2R resident healthy brains wild-type mice or...
Monoacylglycerol lipase (MAGL) is the pivotal catabolic enzyme responsible for signal termination in endocannabinoid system. Inhibition of MAGL offers unique advantages over direct activation cannabinoid receptors treating cancer, metabolic disorders, and inflammatory diseases. Although specific fluorescent molecular imaging probes are commonly used real-time analysis localization distribution drug targets cells, they almost invariably composed a linker connecting pharmacophore with large...
Objective Myasthenia gravis (MG) is the most common autoimmune disorder affecting neuromuscular junction. However, evidence shaping treatment decisions, particularly for treatment-refractory cases, sparse. Both rituximab and eculizumab may be considered as therapeutic options refractory MG after insufficient symptom control by standard immunosuppressive therapies. Methods In this retrospective observational study, we included 57 rituximab-treated 20 eculizumab-treated patients with to...
Time-of-day significantly influences the severity and incidence of stroke. Evidence has emerged not only for circadian governance over stroke risk factors, but also important determinants clinical outcome. In this review, we provide a comprehensive overview interplay between chronobiology cerebrovascular disease. We discuss regulation pathophysiological mechanisms underlying onset or tolerance as well in vascular dementia. This includes cell death mechanisms, metabolism, mitochondrial...
Uracil-DNA glycosylase (UNG) is involved in base excision repair of aberrant uracil residues nuclear and mitochondrial DNA. Ung knockout mice generated by gene targeting are viable, fertile, phenotypically normal have regular mutation rates. However, when exposed to a nitric oxide donor, Ung–/– fibroblasts show an increase the uracil/cytosine ratio genome augmented cell death. After combined oxygen-glucose deprivation, primary cortical neurons increased vulnerability death, which associated...
Uracil-DNA glycosylase (UNG) is involved in base excision repair of aberrant uracil residues nuclear and mitochondrial DNA. Ung knockout mice generated by gene targeting are viable, fertile, phenotypically normal have regular mutation rates. However, when exposed to a nitric oxide donor, Ung–/– fibroblasts show an increase the uracil/cytosine ratio genome augmented cell death. After combined oxygen-glucose deprivation, primary cortical neurons increased vulnerability death, which associated...
The metabolic state of a cell is key determinant in the decision to live and proliferate or die. Consequently, balanced energy metabolism regulation apoptosis are critical for development maintenance differentiated organisms. Hypoxia occurs physiologically during exercise pathologically vascular disease, tumorigenesis, inflammation, interfering with homeostatic metabolism. Here, we show that hypoxia-inducible factor (HIF)-1–regulated glycolytic enzyme hexokinase II (HKII) acts as molecular...
The test methods that currently exist for the identification of thyroid hormone system-disrupting chemicals are woefully inadequate. There no internationally validated in vitro assays, and can capture consequences diminished or enhanced action on developing brain missing entirely. These gaps put public at risk assessors a difficult position. Decisions about status as system disruptors based inadequate toxicity data. ATHENA project (Assays Thyroid Hormone axis-disrupting chemicals:...
Abstract Myasthenic crisis (MC) is a life-threatening condition for patients with myasthenia gravis (MG). Muscle-specific kinase-antibodies (MuSK-ABs) are detected in ~ 6% of MG, but data on outcome MuSK-MCs still lacking. We made subgroup analysis who presented MC either acetylcholine-receptor-antibody positive MG (AchR-MG) or MuSK-MG between 2006 and 2015 retrospective German multicenter study. identified 19 MuSK-AB associated MCs 15 161 144 AchR-ABs only. In contrast to AchR-AB, were more...
Abstract Myasthenic crisis (MC) is a life-threatening condition for patients with myasthenia gravis (MG). Seronegative represent around 10–15% of MG, but data on outcome seronegative MCs are lacking. We performed subgroup analysis who presented MC either acetylcholine-receptor-antibody-positive MG (AChR-MG) or between 2006 and 2015 in retrospective German multicenter study. identified 15 17 142 AChR-MG 159 MCs. were younger (54.3 ± 14.5 vs 66.5 16.3 years; p = 0.0037), had higher rate thymus...
The brain responds to noxious stimulation with protective signalling. Over the last decades, a number of experimental strategies have been established study endogenous protection. Pre-, per-, post- and remote 'conditioning' are now widely used unravel underlying mechanisms neuroprotection. Some these currently being tested in clinical trials protect human against anticipated damage or boost responses during after injury. Here we summarize principles research current efforts translate this...
Background: Mutations of monocarboxylate transporter 8 (MCT8), a thyroid hormone (TH)-specific transmembrane transporter, cause severe neurodevelopmental disorder, the Allan-Herndon-Dudley syndrome. In MCT8 deficiency, TH is not able to reach those areas brain where uptake depends on MCT8. Currently, therapeutic options for MCT8-deficient patients are missing, as treatment successful in improving neurological deficits. Available data protein and transcript levels indicate complex expression...
Phenotypic profiling of large three-dimensional microscopy data sets has not been widely adopted due to the challenges posed by cell segmentation and feature selection. The computational demands automated processing further limit analysis hard-to-segment images such as neurons organoids. Here we describe a comprehensive shallow-learning framework for quantitative phenotyping (3D) image using unsupervised data-driven voxel-based learning, which enables computationally facile classification,...
Background Myasthenia gravis (MG) is a rare autoimmune disease characterized by fatigable weakness of the voluntary muscles and can exacerbate to life-threatening myasthenic crisis (MC), requiring intensive care treatment. Routine laboratory parameters are cost-effective widely available method for estimating clinical outcomes several diseases, but so far, such have not been established detect progression in MG. Methods We conducted retrospective analysis selected related inflammation...