Abstract Background Pyroptosis is a form of proinflammatory gasdermin-mediated programmed cell death. Abnormal mucosal inflammation in the intestine critical risk factor for colitis-associated colorectal cancer (CAC). However, it unknown whether pyroptosis participates development CAC. Methods To investigate role gasdermin E (GSDME)-mediated CAC, Gsdme −/− mice and their wild-type (WT) littermate controls were challenged with azoxymethane (AOM) dextran sodium sulfate (DSS) to induce CAC...

Abstract Background Ketone body β-hydroxybutyrate (BHB) has received more and attentions, because it possesses a lot of beneficial, life-preserving effects in the fields clinical science medicine. However, role BHB intestinal inflammation not yet been investigated. Methods Colonic mucosa inflammatory bowel disease (IBD) patients healthy controls were collected for evaluation level. Besides, therapeutic effect exogenous murine model acute dextran sulfate sodium (DSS)-induced colitis assessed...

Macrophages are a promising therapeutic target for intestinal mucosal repair. MiR-146b appears to control macrophage activation and cell proliferation.By loading miR-146b mimic on mannose-modified trimethyl chitosan [MTC]-conjugated nanoparticles [NPs] [MTC-miR146b], molecular targeted immunotherapeutic approach was developed selectively macrophages regeneration tumourigenesis in mouse models.We first confirmed that expression significantly enhanced during murine colitis model. Moreover，...

Crohn's disease (CD) is a kind of refractory intestinal inflammatory diseases. Pyroptosis was recently identified as gasdermin-mediated proinflammatory cell death. However, it unclear whether pyroptosis participates in the pathogenesis CD. Here, we show that pyroptosis-inducing fragment GSDME N-terminal obviously detected inflamed colonic mucosa but not uninflamed patients with CD, suggesting GSDME-mediated may be correlated mucosal inflammation To investigate role colitis development,...

Short-chain fatty acids (SCFAs) exhibit anticancer activity in cellular and animal models of colon cancer. Acetate, propionate, butyrate are the three major SCFAs produced from dietary fiber by gut microbiota fermentation have beneficial effects on human health. Most previous studies antitumor mechanisms focused specific metabolites or genes involved pathways, such as reactive oxygen species (ROS) biosynthesis. In this study, we performed a systematic unbiased analysis acetate, ROS levels...

Abstract Background The Western dietary pattern, characterized by high consumption of fats and sugars, has been strongly associated with an increased risk developing Crohn’s disease (CD). However, the potential impact maternal obesity or prenatal exposure to a diet on offspring’s susceptibility CD remains unclear. Herein, we investigated effects underlying mechanisms high-fat/high-sugar Western-style (WD) 2,4,6-Trinitrobenzenesulfonic acid (TNBS)-induced Crohn’s-like colitis. Methods...

Scutellaria baicalensis Georgi (SBG), a widely used traditional Chinese medicine, exhibits anti-inflammatory and antioxidant properties. Wogonin is one of the primary bioactive components SBG. Acetaminophen (APAP)-induced liver injury (AILI) represents prevalent form drug-induced damage primarily driven by inflammatory responses oxidative stress.

Mequindox is used as a veterinary antibiotic drug. As part of systematic investigations into mequindox medicine and its subsequent applications in food safety, we conducted the investigation to assess metabolic response mice using metabonomics, which combines NMR profiles biofluids or tissues pattern recognition data analysis. In this study, delivered single dose with dosage levels 15, 75, 350 mg/kg body weight collected urine samples over 7 day period, well plasma liver at days postdose....

Shedding of intestinal epithelial cells [IECs] is a potent cause barrier loss which plays an important role in the pathogenesis inflammatory bowel disease [IBD]. TNFα can induce IEC shedding, but little known about this process.To investigate molecular mechanism regulating mice lacking interferon regulatory factor1 [IRF1], caspase-3, or gasdermin E [GSDME] and their control wild-type [WT] littermates were intravenously injected with tumour necrosis factor alpha [TNFα] to establish shedding...

Abstract Background The interplay between gut microbiota and immune responses is crucial in ulcerative colitis (UC). Though Akkermansia muciniphila (Akk) shows therapeutic potential, the mechanisms remain unclear. This study sought to investigate differences efficacy among different forms or strains of Akk elucidate underlying mechanisms. Results Employing a dextran sulfate sodium (DSS)-induced mouse model, we assessed Akk’s impact on using cellular cytokine analysis, phenotyping,...

Myasthenia gravis (MG) is an antibody-mediated autoimmune disease and its pathogenesis closely related to CD4 + T cells. In recent years, gut microbiota considered play important role in the of MG. Astragaloside IV (AS-IV) one main active components extracted from Astragalus membranaceus has immunomodulatory effects. To study effect AS-IV changes on experimental myasthenia (EAMG) mice, we explore possible mechanism improving MG.In this study, network pharmacology was utilized screen crucial...

Cyadox is an antibiotic drug and has the potential to be used as a feedstuff additive in promoting growth of animals. However, toxicity cyadox should fully assessed before application, this prompted current investigation on metabolic responses mice exposure, using metabonomic technique. Three groups Kunming were respectively given single dose at three different concentrations (100, 650, 4000 mg/kg body weight) via gavage. We present here alterations urine, plasma, liver, renal medulla...

Abstract A simulation analysis and an experiment are carried out to investigate how the gap difference between breaks of a direct current vacuum circuit breakers with multi‐breaks (MB‐DC VCB) caused by mechanism dispersion breaker influences distribution TRV among breaks. An interruption model MB‐DC VCB, combining continuous transition model, is established analyse rising rate transient recovery voltage dielectric strength speed for VCB under different conditions. Based on experimental...

The cathode spot motion characteristics of vacuum arc are closely related to the evolution law arc, and there errors institutional overhead viewpoint leading distortion trajectory through high-speed camera in process information acquisition. Experimentally, it is found that before error compensation, circular morphological features contact surface present ellipse captured image, which indicates acquired contains perspective error; presents pixel vertical drop distribution, motion. Based on...

Mequindox is used as an antibiotic drug in livestock; however, its toxicity remains largely unclear. Previously, we investigated metabolic responses of mice to mequindox exposure. In order evaluate dependences animal species response insult, present the consequences exposure a rat model, by employing combination metabonomics and transcriptomics. Metabolic profiling urine revealed that recovery achieved for rats exposed low or moderate dose mequindox, whereas high levels trigger liver...

To establish a new method for detecting crystal violet (CV), harmful dye, herein, genre of novel biomass carbon dots (CDs) was synthesized via microwave and employed as fluorescent probe, in which water spinach polyethylene glycol (PEG) performed raw materials. Based on the inner filter effect (IFE) between luminescent CDs CV, blue emission this probe at 430 nm could be quenched by CV. Hence, strategy proposed to selectively determine CV aquaculture ambient. Moreover, under optimal...

Lysinibacillus sphaericus is a bacterium incapable of metabolizing sugars with the sole exception N-acetylglucosamine. To unravel regulatory role catabolite control protein A (CcpA) in sugar metabolism L. sphaericus, ccpA deficient mutant was constructed by homologous recombination. The showed growth deficiency and low efficiency carbon energy utilization. NMR spectroscopy combination multivariate data analysis revealed that metabolome dominated 25 metabolites mainly including amino acids,...

Summary Sesquiterpene lactones ( STL s) are C15 terpenoid natural products with α‐methylene γ‐lactone moiety. A large proportion of s in Asteraceae species is derived from the central precursor germacrene acid GAA ). Formation lactone rings depends on regio‐(C6 or C8) and stereoselective (α‐ β‐)hydroxylations , producing four distinct stereo‐configurations (12,6α‐, 12,6β‐, 12,8α‐, 12,8β‐olide derivatives ) nature. Curiously, two configurations (C12,8α C12,8β) simultaneously present Chinese...

Abstract The self-excited DC air circuit breaker (SE-DCCB) has been widely used in urban rail transit due to its excellent stability. It can realize forward and reverse interruption, but difficulty interrupting small currents the phenomenon of arc root sticking at entrance chamber splitting process, which is known as stagnation. A coupling model magnetic field magnetohydrodynamics established for SE-DCCB with traditional structure. field, temperature airflow distribution are investigated an...

A 30-kV direct current vacuum circuit breaker with double-break (DB-DC VCB) in series was taken into consideration; the interrupting and transient recovery voltage (TRV) under both synchronous asynchronous conditions were measured, arcing process during captured by a high-speed camera. The continuous transition model (CTM) used to simulate postarc dielectric characteristic interrupting. experimental simulation results show that when one of breaks DB-DC VCB is delayed 5-kA due dispersion...