A5012819166- Renal Diseases and Glomerulopathies
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Extracellular vesicles in disease
- Immunotherapy and Immune Responses
- Reproductive System and Pregnancy
- Pregnancy and Medication Impact
- Chronic Kidney Disease and Diabetes
- Ferroptosis and cancer prognosis
- Inflammasome and immune disorders
- Chemokine receptors and signaling
- Cancer Immunotherapy and Biomarkers
- Cell Adhesion Molecules Research
- Immune cells in cancer
- Monoclonal and Polyclonal Antibodies Research
- Dialysis and Renal Disease Management
- Pregnancy and preeclampsia studies
- Renal and Vascular Pathologies
- Heme Oxygenase-1 and Carbon Monoxide
- CAR-T cell therapy research
- IL-33, ST2, and ILC Pathways
- Renal and related cancers
- Sustainable Building Design and Assessment
- Muscle and Compartmental Disorders
- Geoscience and Mining Technology
Royal Brisbane and Women's Hospital
2013-2025
Queensland Health
2012-2025
Kidney Health Australia
2013-2019
QIMR Berghofer Medical Research Institute
2015
Tianjin University
2015
Yanshan University
2007
Institute of Forensic Science
1990-1994
Ministry of Public Security of the People's Republic of China
1994
Hypoxia is the key pathobiological trigger of tubular oxidative stress and cell death that drives transition acute kidney injury (AKI) to chronic disease (CKD). The mitochondrial-rich proximal epithelial cells (PTEC) are uniquely sensitive hypoxia thus, pivotal in propagating sustained loss AKI-to-CKD transition. Here, we examined role PTEC-derived small extracellular vesicles (sEV) 'wave death'. Ex vivo patient-derived PTEC were cultured under normoxia (21 % O2) (1 on Transwell inserts for...
Natural killer (NK) cells are a population of lymphoid that play significant role in mediating innate immune responses. Studies mice suggest pathological for NK models kidney disease. In this study, we characterized the cell subsets present native kidneys patients with tubulointerstitial fibrosis, hallmark chronic Significantly higher numbers total (CD3-CD56+) were detected renal biopsies fibrosis compared diseased without and healthy tissue using multi-color flow cytometry. At subset level,...
Dendritic cells (DCs) play critical roles in immune-mediated kidney diseases. Little is known, however, about DC subsets human chronic disease, with previous studies restricted to a limited set of pathologies and using immunohistochemical methods. In this study, we developed novel protocols for extracting renal from diseased kidneys identified, enumerated, phenotyped them by multicolor flow cytometry. We detected significantly greater numbers total DCs as well CD141(hi) CD1c(+) myeloid...
Mucosal-associated invariant T (MAIT) cells represent a specialized lymphocyte population associated with chronic inflammatory disorders. Little is known, however, about MAIT in diseases of the kidney, including CKD.To evaluate human native kidneys tubulointerstitial fibrosis, hallmark CKD, we used multicolor flow cytometry to identify, enumerate, and phenotype such from kidney tissue biopsy samples, immunofluorescence microscopy localize these cells. We cocultured primary proximal tubular...
Abstract Inflammasomes are multiprotein platforms responsible for the release of pro-inflammatory cytokines interleukin (IL)-1β and IL-18. Mouse studies have identified inflammasome activation within dendritic cells (DC) as pivotal driving tubulointerstitial fibrosis inflammation, hallmarks chronic kidney disease (CKD). However, translation this work to human CKD remains limited. Here, we examined complex tubular cell death pathways mediating in DC and, thus, progression. Ex vivo...
Abstract The pathogenesis of crystal nephropathy involves deposition intratubular crystals, tubular obstruction and cell death. 8-dihydroxyadenine (DHA) crystals within kidney tubules, for instance, is caused by a hereditary deficiency adenine phosphoribosyl transferase in humans or overload preclinical models. However, the downstream pathobiological patterns attrition adenine/DHA-induced remain poorly understood. In this study, we investigated: (i) modes adenine-induced death an...
Proximal tubular epithelial cells (PTEC) are central players in inflammatory kidney diseases. However, the complex signalling mechanism/s via which polarized PTEC mediate disease progression poorly understood. Small extracellular vesicles (sEV), including exosomes, recognized as fundamental components of cellular communication and courtesy their molecular cargo (lipids, microRNA, proteins). In this study, we examined content function sEV secreted from apical versus basolateral surfaces human...
ABSTRACT Human proximal tubular epithelial cells (PTEC) of the kidney are known to respond and mediate disease process in a wide range diseases, yet their exosomal production exosome molecular cargo remain mystery. Here we investigate, for first time, content exosomes derived from primary human PTEC cultured under normal diseased conditions representing spectrum vivo severity early inflammation, experienced multiple initial states, through hypoxia, frequently seen late stage chronic (CKD)...
BackgroundWe have previously demonstrated that human kidney proximal tubule epithelial cells (PTEC) are able to modulate autologous T and B lymphocyte responses. It is well established dendritic (DC) responsible for the initiation direction of adaptive immune responses these occur in renal interstitium close apposition PTEC under inflammatory disease settings. However, there no information regarding interaction with DC an context.
Background: Human natural killer (NK) cells are key functional players in kidney transplant rejection. However, the respective contributions of two functionally distinct human NK cell subsets (CD56bright cytokine-producing vs. CD56dim cytotoxic effector) episodes allograft rejection remain uncertain, with current immunohistochemical methods unable to differentiate these discrete populations. We report outcomes an innovative multi-color flow cytometric-based approach unequivocally define and...
Background. Renal proximal tubule epithelial cells (PTEC) respond and contribute to the pathological process in a range of kidney diseases. Within this disease setting, PTEC up-regulate surface antigens which may enable them act as non-professional antigen-presenting become targets for infiltrating T context allograft rejection. In order define, first time, whether modulate immune responses within autologous human system, we monitored their interaction with B presence stimuli mimic...
γδ T cells are effector lymphocytes recognized as key players during chronic inflammatory processes. Mouse studies suggest a pathological role for in models of kidney disease. Here we evaluated human native kidneys with tubulointerstitial fibrosis, the hallmark disease.γδ were extracted from tissue and enumerated phenotyped by multicolour flow cytometry. Localization cytokine production was examined immunofluorescent microscopy.We detected significantly elevated numbers diseased biopsies...
Descriptions of inflammatory cells infiltrating the human kidney rarely mention B cells, other than in specific scenario transplantation. In these reports, are localized almost exclusively within tubulointerstitium where they ideally placed to interact with proximal tubule epithelial (PTEC). We have previously shown that activated PTEC down-modulate autologous T lymphocyte and dendritic cell function. this report, we extend prior studies describe PTEC-B interactions. Stimulated were cultured...
Proximal tubule epithelial cells (PTEC) of the kidney line proximal downstream glomerulus and play a major role in re-absorption small molecular weight proteins that may pass through glomerular filtration process. In perturbed disease state PTEC also contribute to inflammatory process via both positive negative mechanisms production cytokines which chemo-attract leukocytes subsequent down-modulation these prevent uncontrolled responses. It is well established dendritic are responsible for...
Interstitial fibrosis, a histological process common to many kidney diseases, is the precursor state end stage disease, devastating and costly outcome for patient health system. Fibrosis historically associated with chronic disease (CKD) but emerging evidence now linking forms of acute (AKD) development CKD. Indeed, we others have observed at least some degree fibrosis in up 50% clinically defined cases AKD. Epithelial cells proximal tubule (PTEC) are central interstitial fibrosis. We...
Proximal tubular epithelial cells (PTEC) are key players in the progression of kidney diseases. PTEC studies to date have primarily used mouse models and transformed human lines. However, translatability these disease has been questioned. In this study, we investigated phenotypic functional response primary oxidative stress, an established driver disease. Furthermore, examined contribution underlying histopathology cortical tissue generate our PTEC. We demonstrated that from both...
Bile cast nephropathy (BCN) is an underdiagnosed cause of acute kidney injury (AKI). The precise pathogenesis bilirubin tubular toxicity remains unknown. aim this study to explore the cellular and molecular pathophysiology human BCN. Paraffin-embedded sections renal biopsy tissue from a BCN patient were stained by immunohistochemistry (IHC) for oxidative stress (4-hydroxynonenal), immune cell subpopulations, including dendritic cells (CD1c), macrophages (CD68) T (CD3), inflammasome...