A5075684728- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Immunotherapy and Immune Responses
- Computer Graphics and Visualization Techniques
- RNA Interference and Gene Delivery
- Cancer Immunotherapy and Biomarkers
- Cancer-related molecular mechanisms research
- Nanoplatforms for cancer theranostics
- 3D Shape Modeling and Analysis
- Advanced Numerical Analysis Techniques
- Advanced Vision and Imaging
- Teaching and Learning Programming
- Seismic Waves and Analysis
- Augmented Reality Applications
- Nanopore and Nanochannel Transport Studies
- Educational Games and Gamification
- Geographic Information Systems Studies
- Selenium in Biological Systems
- Data Management and Algorithms
- Circular RNAs in diseases
- Constraint Satisfaction and Optimization
- Microfluidic and Bio-sensing Technologies
- Medical Imaging and Pathology Studies
- RNA modifications and cancer
- Glutathione Transferases and Polymorphisms
The University of Queensland
2015-2025
QIMR Berghofer Medical Research Institute
2014-2023
University of Canterbury
2008-2021
Digital Proteomics (United States)
2017
University of Waikato
2013
University of Auckland
1977-2004
Cornell University
1994-2002
Extracellular vesicles represent a rich source of novel biomarkers in the diagnosis and prognosis disease. However, there is currently limited information elucidating most efficient methods for obtaining high yields pure exosomes, subset extracellular vesicles, from cell culture supernatant complex biological fluids such as plasma. To this end, we comprehensively characterize variety exosome isolation protocols their efficiency, yield purity isolated exosomes. Repeated ultracentrifugation...
Small membranous secretions from tumor cells, termed exosomes, contribute significantly to intercellular communication and subsequent reprogramming of the microenvironment. Here, we use optical imaging determine that exogenously administered fluorescently labeled exosomes derived highly metastatic murine breast cancer cells distributed predominantly lung syngeneic mice, a frequent site metastasis. At sites accumulation, were taken up by CD45+ bone marrow-derived cells. Subsequent long-term...
Tumor-derived exosomes are being recognized as essential mediators of intercellular communication between cancer and immune cells. It is well established that bone marrow-derived macrophages (BMDMs) take up tumor-derived exosomes. However, the functional impact these on macrophage phenotypes controversial not studied. Here, we show breast cancer-derived alter phenotype through Interleukin 6 (IL-6) receptor beta (glycoprotein 130, gp130)-STAT3 signaling pathway. Addition to results in...
Small extracellular vesicles (sEVs) provide major promise for advances in cancer diagnostics, prognostics, and therapeutics, ascribed to their distinctive cargo reflective of pathophysiological status, active involvement intercellular communication, as well ubiquity stability bodily fluids. As a result, the field sEV research has expanded exponentially. Nevertheless, there is lack standardisation methods isolation from cells grown serum-containing media. The majority researchers use media...
Metastatic spread of a cancer to secondary sites is coordinated, non-random process. Cancer cell-secreted vesicles, especially exosomes, have recently been implicated in the guidance metastatic dissemination, with specific surface composition determining some aspects organ-specific localization. Nevertheless, whether tumor microenvironment influences exosome biodistribution has yet be investigated. Here, we show that microenvironmental cytokines, particularly CCL2, decorate exosomes via...
To render images from a three-dimensional array of sample values, it is necessary to interpolate between the samples. This paper concerned with interpolation methods that are equivalent convolving samples reconstruction filter; this covers all commonly used schemes, including trilinear and cubic interpolation. We first outline formal basis in signal processing theory. then propose numerical metrics can be measure filter characteristics relevant appearance generated using filter. apply those...
Non-small cell lung cancer (NSCLC) is the most common type and cause of mortality in patients. NSCLC often associated with resistance to chemotherapeutics together rapid metastatic spread, results limited treatment options poor patient survival. NSCLCs are heterogeneous, consist epithelial mesenchymal cells. Mesenchymal cells thought be responsible for chemoresistance phenotype, but if how this phenotype can transferred other currently not known. We hypothesised that small extracellular...
Abstract A manner in which cells can communicate with each other is via secreted nanoparticles termed exosomes. These vesicles contain lipids, nucleic acids, and proteins, are said to reflect the cell‐of‐origin. However, for exosomal protein content, there limited evidence literature verify this statement. Here, proteomic assessment combined pathway‐enrichment analysis used demonstrate that cargo of exosomes reflects epithelial/mesenchymal phenotype secreting breast cancer cells. Given...
How should we assess programming skills? Asking students to write code in a traditional hand-written exam can produce results like those Figure 1. It is nearly impossible meaningfully grade such code. With sufficient effort one get some idea of whether the general correct, but skill need much more than this. For example, there will almost certainly be errors code; how do know student would able correct or not?
To render images from a three-dimensional array of sample values, it is necessary to interpolate between the samples. This paper concerned with interpolation methods that are equivalent convolving samples reconstruction filter; this covers all commonly used schemes, including trilinear and cubic interpolation. We first outline formal basis in signal processing theory. then propose numerical metrics can be measure filter characteristics relevant appearance generated using filter. apply those...
Abstract Exosomes are cell‐derived vesicles secreted by both normal and cancerous cells into the extracellular matrix in blood circulation. Tumor‐derived exosomes have attracted increasing attention noninvasive cancer diagnosis prognosis. However, their effective capture specific detection pose significant technical challenges. Current methods largely fail to quantify tumor‐derived present total (bulk) exosome population derived from body fluids of patients. In this proof‐of‐concept study,...
Abstract Purpose: To study the response of irradiated and out-of-field normal tissues during localized curative intent radiotherapy. Experimental Design: Sixteen patients with non–small cell lung carcinoma (NSCLC) received 60 Gy in 30 fractions definitive thoracic radiotherapy or without concurrent chemotherapy. Peripheral blood lymphocytes (PBL) eyebrow hairs were sampled prior, during, after Clinical variables dose/volume, patient age, use chemoradiotherapy tested for association γ-H2AX...
Accurate identification of malignant lung lesions is a prerequisite for rational clinical management to reduce morbidity and mortality cancer. However, classification nodules into benign cases difficult as they show similar features in computer tomography sometimes positron emission imaging, making invasive tissue biopsies necessary. To address the challenges evaluating indeterminate nodules, authors investigate molecular profiles small extracellular vesicles (sEVs) differentiating via...
Immune checkpoint proteins (ICPs) play a major role in patient's immune response against cancer. Tumour cells usually express those to communicate with as process of escaping the anti-cancer response. Detecting functional present on cancer (such circulating tumor or CTCs) and examining heterogeneity their expression at single-cell level could crucial both diagnosis monitoring therapy. In this study, we develop mesoporous gold biosensor precisely assess ICP individual within lung model. The...
Abstract Glycosylation is the most common post‐translational modification of proteins and regulates a myriad fundamental biological processes under normal, pathological conditions. Altered protein glycosylation linked to malignant transformation, showing distinct glycopatterns that are associated with cancer initiation progression by regulating tumor proliferation, invasion, metastasis, therapeutic resistance. The small extracellular vesicles (sEVs) released cells promising candidates for...
Abstract Lung cancer is responsible for the highest rate of mortality worldwide. patients are often ineligible tumor biopsies due to comorbidities. As a result, may not have most effective treatment regimens administered. Patients with mutations in epidermal growth factor receptor (EGFR) improved survival response EGFR tyrosine kinase inhibitors. A noninvasive method determining would promising clinical applications. Exosomes potential be novel diagnostic markers cancer. Using MS analysis,...
Abstract Despite significant therapeutic advances, lung cancer remains the leading cause of cancer-related death worldwide 1 . Non-small cell (NSCLC) patients have a very poor overall five-year survival rate only 10–20%. Currently, TNM staging is gold standard for predicting and selecting optimal initial treatment options NSCLC patients, including those with curable stages disease. However, many locoregionally-confined relapse die despite curative-intent interventions, indicating need...
Identifying small extracellular vesicle (sEV) subpopulations based on their different molecular signatures could potentially reveal the functional roles in physiology and pathology. However, it is a challenge to achieve this aim due nano-sized dimensions of sEVs, low quantities biological cargo each sEV carries, our incomplete knowledge identifying features capable separating heterogeneous subpopulations. Here, sensitive, multiplexed, nano-mixing-enhanced subpopulation characterization...
ABSTRACT Human proximal tubular epithelial cells (PTEC) of the kidney are known to respond and mediate disease process in a wide range diseases, yet their exosomal production exosome molecular cargo remain mystery. Here we investigate, for first time, content exosomes derived from primary human PTEC cultured under normal diseased conditions representing spectrum vivo severity early inflammation, experienced multiple initial states, through hypoxia, frequently seen late stage chronic (CKD)...
The implementation of accurate and sensitive molecular detection for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is paramount to effectively control ongoing disease 2019 (COVID-19) pandemic. In this regard, we herein propose specific highly SARS-CoV-2 based on nanoyeast single-chain-variable fragment (scFv) ultrasensitive plasmonic nanobox-integrated nanomixing microassay. Importantly, designed platform showcases utility nanoyeast-scFvs as capture reagents targeting...