A5084173881- Effects of Radiation Exposure
- Radiation Therapy and Dosimetry
- DNA Repair Mechanisms
- Carcinogens and Genotoxicity Assessment
- Cancer Cells and Metastasis
- Advanced Radiotherapy Techniques
- Cancer Immunotherapy and Biomarkers
- Cancer Genomics and Diagnostics
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer Research and Treatments
- Lung Cancer Diagnosis and Treatment
- Immune Cell Function and Interaction
- Cancer therapeutics and mechanisms
- Advances in Oncology and Radiotherapy
- Lung Cancer Treatments and Mutations
- Circular RNAs in diseases
- Hematopoietic Stem Cell Transplantation
- Radiation Effects and Dosimetry
- Ultrasound and Hyperthermia Applications
- Immune Response and Inflammation
- Posttraumatic Stress Disorder Research
- bioluminescence and chemiluminescence research
- Anesthesia and Neurotoxicity Research
- Telomeres, Telomerase, and Senescence
- Extracellular vesicles in disease
University of Bern
2021-2024
University of Wollongong
2023-2024
Peter MacCallum Cancer Centre
2014-2023
The University of Melbourne
2014-2023
Albany Medical Center Hospital
2023
Consorcio Hospitalario Provincial de Castellón
2020
National Institutes of Health
2003-2016
National Cancer Institute
2003-2016
Centre de Recherche sur les Liens Sociaux
2016
Centre National de la Recherche Scientifique
2016
Current advances in cancer biology have identified major pathways involved tumorigenesis. The association of DNA damage with premalignant stages tumor progression, genome instability and further oncogenic transformation opens the possibility using common markers for early detection, prediction, prognosis, therapeutics possibly prevention. Perhaps most sensitive marker is γ-H2AX formation chromatin flanking free double-stranded ends double-strand breaks (DSBs) eroded telomeres, both present...
The past year has seen considerable developments in the use of DNA double-strand breaks (DSBs) to evaluate genome alterations cells undergoing a variety genotoxic stresses vitro and vivo. When γ -H2AX foci which mark DSBs are stained, individual detectible, making assay suitable for situations requiring great sensitivity. While methods detection still evolving, particularly vivo detection, basic proven be useful several diverse areas research. We will highlight recent four areas: radiation...
Detrimental effects of ionizing radiation (IR) are correlated to the varying efficiency IR induce complex DNA damage. A double strand break (DSB) can be considered simpler form These types damage consist DSBs, single breaks (SSBs) and/or non-DSB lesions such as base damages and apurinic/apyrimidinic (AP; abasic) sites in different combinations. Enthralling theoretical (Monte Carlo simulations) experimental evidence suggests an increase complexity therefore repair resistance with linear...
Abstract Although microbial infections can alter steady-state hematopoiesis, the mechanisms that drive such changes are not well understood. We addressed a role for IFN-γ signaling in infection-induced bone marrow suppression and anemia murine model of human monocytic ehrlichiosis, an emerging tick-borne disease. Within Ehrlichia muris-infected C57BL/6 mice, we observed reduction myeloid progenitor cells, as defined both phenotypically functionally. Infected mice exhibited concomitant...
Purpose Lung inflammation leading to pulmonary toxicity after radiotherapy (RT) can occur in patients with non-small cell lung cancer (NSCLC). We investigated the kinetics of RT induced plasma inflammatory cytokines these order identify clinical predictors toxicity. Experimental Design In 12 NSCLC patients, 60 Gy (30 fractions over 6 weeks) was delivered; received concurrent chemoradiation (chemoRT) and alone. Blood samples were taken before therapy, at 1 24 hours delivery 1st fraction, 4...
How hematopoietic stem cells (HSCs) respond to inflammatory signals during infections is not well understood. Our studies have used a murine model of ehrlichiosis, an emerging tick-born disease, address how infection impacts function. Infection C57BL/6 mice with the intracellular bacterium, Ehrlichia muris, results in anemia and thrombocytopenia, similar what observed human ehrlichiosis patients. In mouse, promotes myelopoiesis, process that critically dependent on interferon gamma (IFNγ)...
Abstract Purpose: To study the response of irradiated and out-of-field normal tissues during localized curative intent radiotherapy. Experimental Design: Sixteen patients with non–small cell lung carcinoma (NSCLC) received 60 Gy in 30 fractions definitive thoracic radiotherapy or without concurrent chemotherapy. Peripheral blood lymphocytes (PBL) eyebrow hairs were sampled prior, during, after Clinical variables dose/volume, patient age, use chemoradiotherapy tested for association γ-H2AX...
Ionizing radiation–induced DNA double-strand breaks (DSBs) can lead to cell death, genome instability, and carcinogenesis. Immunofluorescence detection of phosphorylated histone variant H2AX (γ‐H2AX) is a reliable sensitive technique monitor external-beam ionizing DSBs in peripheral blood lymphocytes (PBLs). Here, we investigated whether γ-H2AX could be used as an vivo marker assess normal-tissue toxicity after extended internal irradiation with <sup>177</sup>Lu-DOTA-octreotate (LuTate)...
Younger individuals are more prone to develop cancer upon ionizing radiation (IR) exposure. Radiation-induced tumors associated with inefficient repair of IR-induced DNA damage and genome instability. Phosphorylation histone H2AX (γ-H2AX) is the initial event in on chromatin flanking strand breaks. This step crucially important for breaks maintenance stability. We studied molecular underpinnings age-related IR effects using an animal model. By assaying γ-H2AX foci we analyzed induction...