A5032691888- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Microfluidic and Bio-sensing Technologies
- Extracellular vesicles in disease
- Electrochemical Analysis and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Epigenetics and DNA Methylation
- Electrochemical sensors and biosensors
- Advanced Nanomaterials in Catalysis
- MicroRNA in disease regulation
- Electrohydrodynamics and Fluid Dynamics
- Advanced Biosensing Techniques and Applications
- Cancer Genomics and Diagnostics
- Monoclonal and Polyclonal Antibodies Research
- Plant Pathogenic Bacteria Studies
- RNA modifications and cancer
- Molecular Junctions and Nanostructures
- 3D Printing in Biomedical Research
- Ionic liquids properties and applications
- Analytical Chemistry and Sensors
- RNA Interference and Gene Delivery
- SARS-CoV-2 detection and testing
- Nanocluster Synthesis and Applications
- Advanced Chemical Sensor Technologies
- RNA and protein synthesis mechanisms
Charles Sturt University
2023-2025
Griffith University
2016-2024
Queensland Theological College
2019
Queensland Centre for Mental Health Research
2019
Institute of Micro and Nanotechnology
2017-2019
The University of Queensland
2012-2018
Brisbane School of Theology
2016-2018
Umm al-Qura University
2017
The University of Melbourne
2016
Monash University
2009-2015
Most of the current exosome-analysis strategies are time-consuming and largely dependent on commercial extraction kit-based preisolation step, which requires extensive sample manipulations, costly isolation kits, reagents, tedious procedures, sophisticated equipment is prone to bias/artifacts. Herein we introduce a simple method for direct subsequent detection specific population exosomes using an engineered superparamagnetic material with multifunctional properties, namely, gold-loaded...
Exosomes show promise as noninvasive biomarkers for cancer, but their effective capture and specific detection is a significant challenge. Herein, we report multiplexed microfluidic device highly of multiple exosome targets using tunable alternating current electrohydrodynamic (ac-EHD) methodology, referred to nanoshearing. In our system, electrical body forces generated by ac-EHD act within nanometers an electrode surface (i.e., the layer) generate nanoscaled fluid flow that enhances...
In this review, we have summarised the biogenesis, biological significance, isolation and detection technologies of four widely known circulating biomarkers namely tumour cells, tumor specific DNA, microRNA, exosomes.
Abstract Epigenetic reprogramming in cancer genomes creates a distinct methylation landscape encompassing clustered at regulatory regions separated by large intergenic tracks of hypomethylated regions. This that we referred to as Methylscape is displayed most types, thus may serve universal biomarker. To-date research has focused on the biological consequences DNA changes whereas its impact physicochemical properties remains unexplored. Herein, examine effect levels and genomic distribution...
Sequence-dependent non-thiolated DNA–gold affinity interaction for biosensing applications, and the underlying principles governing these interactions are comprehensively reviewed.
Current amplification-based microRNA (miRNA) detection approaches are limited by the small sizes of miRNAs as well amplification bias/artifacts. Herein, we report on an amplification-free miRNA assay based elevated affinity interaction between polyadenylated and bare gold electrode. The poly(A) extension 3′ ends magnetically isolated targets facilitated high adsorption efficiency onto electrode surfaces for electrochemical without any cumbersome surface functionalization procedures. showed...
Abstract Tumor-derived exosomes possess significant clinical relevance due to their unique composition of genetic and protein material that is representative the parent tumor. Specific isolation as well identification proportions these clinically relevant (CREs) from biological samples could help better understand significance cancer biomarkers. Herein, we present a simple approach for quantification proportion CREs within bulk exosome population isolated patient serum. This can potentially...
Tumor-derived exosomes have emerged as promising cancer biomarkers due to their unique composition and functions. Herein, we report a stripping voltammetric immunoassay for the electrochemical detection of disease-specific using quantum dots signal amplifiers. The assay involves three subsequent steps where bulk exosome populations are initially magnetically captured on magnetic beads by generic tetraspanin antibody (e.g., CD9 or CD63) followed identification cancer-related. Here, used CdSe...
The enzyme-mimicking activity of iron oxide based nanostructures has provided a significant advantage in developing advanced molecular sensors for biomedical and environmental applications. Herein, we introduce the horseradish peroxidase (HRP)-like gold-loaded nanoporous ferric nanocubes (Au–NPFe2O3NC) development sensor with enhanced electrocatalytic colorimetric (naked eye) detection autoantibodies. results showed that Au–NPFe2O3NC exhibits peroxidase-like toward catalytic oxidation...
Peroxidase-mimetic activity of mesoporous Fe<sub>2</sub>O<sub>3</sub>nanomaterials in global DNA methylation detection using naked eye and electrochemical readout.
A three-dimensional (3D) tissue model has significant advantages over the conventional two-dimensional (2D) model. 3D mimics relevant in-vivo physiological conditions, allowing a cell culture to serve as an effective tool for drug discovery, engineering, and investigation of disease pathology. The present reviews highlight recent advances development microfluidics based methods generation spheroids. paper emphasizes on application microfluidic technology engineering including formation...
Implantable electronics are of great interest owing to their capability for real-time and continuous recording cellular–electrical activity. Nevertheless, as such systems involve direct interfaces with surrounding biofluidic environments, maintaining long-term sustainable operation, without leakage currents or corrosion, is a daunting challenge. Herein, we present thin, flexible semiconducting material system that offers attractive attributes in this context. The consists crystalline cubic...
A sensitive and amplification-free assay for the electrochemical detection of exosomal miRNAs in complex biological samples.