A5002839631- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Immune Response and Inflammation
- Advanced biosensing and bioanalysis techniques
- Immunotherapy and Immune Responses
- 3D Printing in Biomedical Research
- Ultrasound and Hyperthermia Applications
- NF-κB Signaling Pathways
- Metalloenzymes and iron-sulfur proteins
- CRISPR and Genetic Engineering
- Nanoplatforms for cancer theranostics
- Nanoparticle-Based Drug Delivery
- Cell Adhesion Molecules Research
- Photoreceptor and optogenetics research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Biomedical and Engineering Education
- Retinoids in leukemia and cellular processes
- Advanced Fluorescence Microscopy Techniques
- Glycosylation and Glycoproteins Research
- Hydrogen Storage and Materials
- bioluminescence and chemiluminescence research
- Graphene and Nanomaterials Applications
- Antimicrobial Peptides and Activities
- Cell Image Analysis Techniques
- Microfluidic and Capillary Electrophoresis Applications
Kyoto University
2016-2025
Kyoto Pharmaceutical University
2013-2025
Sado General Hospital
2017
Japan Science and Technology Agency
2010-2014
Osaka University
2013
The use of fluorescent proteins has revolutionized our understanding biological processes. However, the requirement for external illumination precludes their universal application to study processes in all tissues. Although light can be created by chemiluminescence, emission from existing chemiluminescent probes is too weak this imaging modality situations when fluorescence cannot used. Here we report development brightest luminescent protein date, Nano-lantern, which a chimera enhanced...
Chitosan has the potential to be a biocompatible gene carrier. However, transfection efficiency of chitosan is low because slow endosomal escape rate. The buffering capacity histidine in pH range would help plasmid DNA (pDNA) from endosomes. In this study, was introduced into improve efficiency. and were linked by disulfide bonds provided 2-iminothiolane cysteine. complexes prepared mixing or histidine-modified with DNA. A broader observed, cellular uptake chitosan/pDNA higher than that...
Occult nodal metastases increase the risk of cancer recurrence, demoting prognosis and quality life patients. While targeted drug delivery by using systemically administered nanocarriers can potentially control metastatic disease, lymph node have been mainly dealt locally injecting nanocarriers, which may not always be applicable. Herein, we demonstrated that sub-50 nm polymeric micelles incorporating platinum anticancer drugs could target in a syngeneic melanoma model after systemic...
The development of optical methods to control cellular functions is important for various biological applications. In particular, heat shock promoter-mediated gene expression systems by laser light are attractive targets controlling functions. However, previous approaches have considerable technical limitations related their use UV, short-wavelength visible (vis), and infrared (IR) light, which poor penetration into tissue. Biological tissue relatively transparent inside the diagnostic...
Transposons are promising systems for somatic gene integration because they can not only integrate exogenous genes efficiently, but also be delivered to a variety of organs using range transfection methods. piggyBac (PB) transposon has high transposability in mammalian cells vitro, and been used genetic preclinical studies. However, the PB vivo demonstrated yet. Here, we PB-mediated sustained expression adult mice. We constructed PB-based plasmid DNA (pDNA) containing reporter [firefly...
Inhalation of bacterial endotoxin induces pulmonary inflammation by activation nuclear factor κB (NFκB), production cytokines and chemokines, neutrophil activation. Although glucocorticoids are the drugs choice, administration free results in adverse effects as a result lack selectivity for inflammatory effector cells. Because alveolar macrophages play key role response lung, selective targeting to offers efficacious pharmacological intervention with minimal side effects. We have...
Hepatitis is often associated with the overexpression of various adhesion molecules. In particular, intracellular molecule-1 (ICAM-1), which expressed on hepatic endothelial cells (HECs) in early stage inflammation, involved serious illnesses. Therefore, ICAM-1 suppression HECs enables inflammatory responses. Here, we developed an small interfering RNA (siRNA) transfer method using ultrasound (US)-responsive and mannose-modified liposome/ICAM-1 siRNA complexes (Man-PEG(2000) bubble...
Charge-neutralized lipid envelope-type nanoparticles formed with SS-cleavable and pH-activated lipid-like materials (ssPalm) accumulate rapidly in the liver without forming aggregates blood circulation, result a liver-specific gene expression for long duration (>2 weeks) neither immunological responses nor hepatotoxicity after intraveneous administration, when it carries pDNA free from CpG-motifs.
Tumor-associated macrophages (TAMs) have an alternatively activated macrophage phenotype (M2) and promote cancer cell proliferation, angiogenesis metastasis. Nuclear factor-kappaB (NF-κB) is one of the master regulators polarization. Here, we investigated effect inhibition NF-κB activity by small interfering RNA (siRNA) on pro-tumor response located in tumor microenvironment vitro. We used mouse peritoneal cultured conditioned medium from colon-26 cells as vitro TAM model (M2-like...
In this study, stable nano-sized bubbles (nanobubbles [NBs]) were produced using the mechanical agitation method in presence of perfluorocarbon gases. NBs made with perfluoropropane had a smaller size (around 400 nm) compared to that those perfluorobutane or nitrogen gas. The lipid concentration affected both their initial and post-formulation stability. formed final 0.5 mg/ml tended be more stable, having uniform distribution for 24 h at room temperature 50 4 °C. vitro gene expression...
A fluorescent thymine analogue, ThexT, enables visualisation of immunostimulatory CpG oligonucleotides in macrophages. Its incorporation site affects TLR9 activation, offering insights into immune response modulation and intracellular DNA tracking.