A5016141607- bioluminescence and chemiluminescence research
- Advanced Fluorescence Microscopy Techniques
- Psoriasis: Treatment and Pathogenesis
- Asthma and respiratory diseases
- ATP Synthase and ATPases Research
- Urticaria and Related Conditions
- Advanced Biosensing Techniques and Applications
- Folate and B Vitamins Research
- Immune Response and Inflammation
- Elbow and Forearm Trauma Treatment
- Contact Dermatitis and Allergies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cardiac tumors and thrombi
- Infectious Diseases and Mycology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Inflammasome and immune disorders
- Sulfur-Based Synthesis Techniques
- Ocular Diseases and Behçet’s Syndrome
- Water Quality Monitoring Technologies
- Soil and Unsaturated Flow
- Autoimmune and Inflammatory Disorders
- Metabolism and Genetic Disorders
- Landslides and related hazards
- Vascular Tumors and Angiosarcomas
- Autoimmune Bullous Skin Diseases
Fujita Health University
2017-2025
Fujita Health University Hospital
2018-2022
The University of Tokyo
2002-2020
Showa Pharmaceutical University
2020
Osaka University
2012-2015
Bunkyo University
2015
Kyoto University
2013
Hokkaido University
1998-2012
Nihon University
2012
Nikon (United States)
2007-2009
Adenosine 5'-triphosphate (ATP) is the major energy currency of cells and involved in many cellular processes. However, there no method for real-time monitoring ATP levels inside individual living cells. To visualize levels, we generated a series fluorescence resonance transfer (FRET)-based indicators that were composed epsilon subunit bacterial F(o)F(1)-ATP synthase sandwiched by cyan- yellow-fluorescent proteins. The indicators, named ATeams, had apparent dissociation constants ranging...
The use of fluorescent proteins has revolutionized our understanding biological processes. However, the requirement for external illumination precludes their universal application to study processes in all tissues. Although light can be created by chemiluminescence, emission from existing chemiluminescent probes is too weak this imaging modality situations when fluorescence cannot used. Here we report development brightest luminescent protein date, Nano-lantern, which a chimera enhanced...
Fluorescence live imaging has become an essential methodology in modern cell biology. However, fluorescence requires excitation light, which can sometimes cause potential problems, such as autofluorescence, phototoxicity, and photobleaching. Furthermore, combined with recent optogenetic tools, the light illumination trigger their unintended activation. Because luminescence does not require it is a good candidate alternative modality to circumvent these problems. The application of imaging,...
Abstract Synthesis of heteroatom‐containing ladder‐type π‐conjugated molecules was successfully achieved via a palladium‐catalyzed intramolecular oxidative CH/CH cross‐coupling reaction. This reaction provides variety bearing heteroatoms, such as nitrogen, oxygen, phosphorus, and sulfur atoms, carbonyl group. The were synthesized efficiently, even in gram scale, larger also obtained by double successive cycloaromatization.
Most triacylglycerol-lowering fibrates have been developed in the 1960s-1980s before their molecular target, peroxisome proliferator-activated receptor alpha (PPARα), was identified. Twenty-one ligand-bound PPARα structures deposited Protein Data Bank since 2001; however, binding modes of and physiological ligands remain unknown. Here we show thirty-four X-ray crystallographic ligand-binding domain, which are composed a "Center" four "Arm" regions, complexes with five endogenous fatty acids,...
Among 330 vibration-exposed workers, 24 cases of hypothenar hammer syndrome in 29 hands were diagnosed by arteriography. The right hand was involved 13, the left six, and both five cases. mean age 55 years, duration vibration exposure 19.4 episodic Raynaud’s phenomenon 6.4 years. vascular lesions ulnar arteries classified into three major types with subtypes. Type 1: stenosis superficial palmar arch around hook hamate. 2a: occlusion 2b: deep arches 3a: artery at proximal part wrist. 3b: near...
Abstract Loss-of-function mutations in the interleukin (IL)-36 gene IL36RN are associated with psoriasis. The importance of neutrophil extracellular traps (NETs), web-like structures composed DNA, pathogenesis psoriasis has been unclear. Here, we aimed to clarify role NET signaling deficiency IL36 receptor antagonist (DITRA). We evaluated severity psoriasis-like lesions induced by imiquimod cream treatment Il36rn −/− mice. mRNA levels psoriasis-related cytokines were measured via real-time...
Loss-of-function homozygous or compound heterozygous mutations in IL36RN, which encodes interleukin-36 receptor antagonist (IL-36Ra), have been implicated the pathogenesis of various skin disorders. Previous findings showed that IL-36γ promoted wound healing mice; however, pathogenic role IL-36Ra remains unclear. We elucidated IL-36Ra, a regulator IL-36 tissue repair by investigating recruitment inflammatory cells and cytokine production absence IL-36Ra. Full-thickness excisional wounds were...
ABSTRACT Antinuclear matrix protein 2 (NXP‐2) antibodies are dermatomyositis‐specific that target NXP‐2 involved in transcriptional regulation. However, their clinical significance and relationship with disease activity remain unclear. We performed a longitudinal examination on serum samples from three patients dermatomyositis who tested positive for anti‐NXP‐2 antibodies. These were analyzed alongside parameters, including activity, muscle enzyme levels, treatment response. The levels of...
Abstract Loss-of-function homozygous or compound heterozygous mutations in IL36RN , which encodes interleukin-36 receptor antagonist (IL-36Ra), have been implicated the pathogenesis of skin disorders. We previously reported that Il36rn −/− mice exhibit an enhanced contact hypersensitivity (CHS) response through increased neutrophil recruitment. In addition, show severe imiquimod-induced psoriatic lesions and extracellular trap (NET) formation. hypothesized NETs may play important role CHS...
Loss-of-function homozygous or compound heterozygous mutations in IL36RN, which encodes interleukin-36 receptor antagonist (IL-36Ra), has been implicated the pathogenesis of skin disorders. However, pathogenic role IL-36Ra cutaneous ischemia-reperfusion (I/R) injury remains unclear.We investigated IL36Ra I/R injury.We examined Il36rn-/- mice. The area wounds, numbers infiltrated cells, apoptotic cells and neutrophil extracellular trap (NET) formation were assessed. expression levels various...
Abstract Loss-of-function mutations in IL36RN cause generalized pustular psoriasis (GPP), which is characterized by neutrophil-infiltrated lesions. Neutrophils are important during contact hypersensitivity mice. However, it has never been determined whether interleukin-36 receptor antagonist (IL-36Ra) deficiency an exacerbating factor dermatitis. We examined a loss-of-function mutation exacerbates dermatitis and evaluated the changes dermatitis-related cytokines. Wild-type Il36rn −/− mice...
Abstract Combination therapy with BRAF and MEK inhibitors (BRAFi/MEKi) have dramatically improved prognosis among patients BRAF‐mutant metastatic melanoma compared traditional treatment, such as chemotherapy. However, resistance to these targeted agents occurs invariably, thereby limiting their clinical efficacy. Recently, it has been reported that the ligand‐independent phosphorylation of erythropoietin‐producing hepatocellular receptor A2 (EphA2) at Ser‐897 signaling is a driver inhibitor...
One of the possible causes phantom limb pain (PLP), which have not been clarified medically, is said that a brain unable to adapt loss limbs. Even though sends signal move limbs, feedback on given because limbs sent are no longer in existence. Recently, some kinds treatment introduced, making patients feel their existence and they able actually by own will, but each them has difficulty. In this research, we propose method PLP using an optical see-through Head Mounted Display (HMD) can...