A5007784791- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Viral Infections and Outbreaks Research
- Vector-Borne Animal Diseases
- Fire effects on ecosystems
- Plant Virus Research Studies
- SARS-CoV-2 and COVID-19 Research
- HIV Research and Treatment
- COVID-19 Clinical Research Studies
- Hepatitis C virus research
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Viral gastroenteritis research and epidemiology
- interferon and immune responses
- Hepatitis B Virus Studies
- Immunotherapy and Immune Responses
- Cellular transport and secretion
- Energy and Environment Impacts
- Animal Virus Infections Studies
- Systemic Lupus Erythematosus Research
- Lipid Membrane Structure and Behavior
- Virology and Viral Diseases
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 detection and testing
- Infectious Encephalopathies and Encephalitis
Université Claude Bernard Lyon 1
2019-2025
École Pratique des Hautes Études
2019-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Infections Virales et Pathologie Comparée
2019-2024
Virologie et Pathologies Humaines
2020-2024
Université Paris Sciences et Lettres
2024
University Hospital Heidelberg
2015-2023
Heidelberg University
2015-2023
Ekaterinburg Research Institute of Viral Infections
2019-2022
CellGenix (Germany)
2021
SARS-CoV-2 is a newly emerged coronavirus that caused the global COVID-19 outbreak in early 2020. primarily associated with lung injury, but many other clinical symptoms such as loss of smell and taste demonstrated broad tissue tropism virus. Early SARS-CoV-2-host cell interactions entry mechanisms remain poorly understood. Investigating infection culture, we found protease TMPRSS2 determines pathway used by In presence TMPRSS2, proteolytic process was completed at plasma membrane, virus...
The hepatitis C virus (HCV) genome codes for highly mannosylated envelope proteins, which are naturally retained in the endoplasmic reticulum. We found that HCV glycoprotein E2 binds dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) and related liver endothelial cell lectin L-SIGN through high-mannose N-glycans. Competing ligands such as mannan an antibody directed against carbohydrate recognition domains (CRD) abrogated binding. While no interaction...
Dengue virus (DV) is a mosquito-borne flavivirus that causes hemorrhagic fever in humans. In the natural infection, DV introduced into human skin by an infected mosquito vector where it believed to target immature dendritic cells (DCs) and Langerhans (LCs). We found productively infects DCs but not LCs. show here interactions between E protein, sole mannosylated glycoprotein present on particles, C-type lectin cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN)...
Dengue virus envelope protein (E) contains two N-linked glycosylation sites, at Asn-67 and Asn-153. The site position 153 is conserved in most flaviviruses, while the 67 thought to be unique for dengue viruses. oligosaccharide side chains on flavivirus E proteins have been associated with viral morphogenesis, infectivity, tropism. Here, we examined relevance of each glycan by removing context infectious particles. viruses lacking were able infect mammalian cells translate replicate genome,...
The molecular mechanisms involved in the hepatic tropism of hepatitis C virus (HCV) have not been identified. We shown previously that liver-expressed C-type lectins L-SIGN and DC-SIGN bind HCV E2 glycoprotein with high affinity (Lozach, P. Y., Lortat-Jacob, H., de Lacroix Lavalette, A., Staropoli, I., Foung, S., Amara, Houles, C., Fieschi, F., Schwartz, O., Virelizier, J. L., Arenzana-Seisdedos, Altmeyer, R. (2003) Biol. Chem. 278, 20358-20366). To analyze functional relevance this...
Hybrid plasmonic-magnetic nanoparticles possess properties that are attractive in bioimaging, targeted drug delivery, vivo diagnosis and therapy. The stability toxicity, however, of such challenge their safe use today. Here, biocompatible, SiO2-coated, Janus-like Ag/Fe2O3 prepared by one-step, scalable flame aerosol technology. A nanothin SiO2 shell around these multifunctional leaves intact morphology, magnetic plasmonic but minimizes the release toxic Ag+ ions from nanosilver surface its...
Severe fever with thrombocytopenia syndrome (SFTS) virus (SFTSV) is an emerging tick-borne that carries a high fatality rate of 12%-50%. In-depth understanding the SFTSV-induced pathogenesis mechanism critical for developing effective anti-SFTS therapeutics. Here, we report transcriptomic analysis blood samples from SFTS patients. We observe strong correlation between inflammatory responses and disease progression fatal outcome. Quantitative proteomic SFTSV infection confirms induction...
ABSTRACT The C-type lectin DC-SIGN expressed on immature dendritic cells (DCs) captures human immunodeficiency virus (HIV) particles and enhances the infection of CD4 + T cells. This process, known as trans -enhancement T-cell infection, has been related to HIV endocytosis. It proposed that targets a nondegradative compartment within DCs DC-SIGN-expressing cells, allowing incoming persist for several days before infecting target In this study, we provide lines evidence suggesting...
The arenavirus Lassa virus (LASV) causes a severe hemorrhagic fever with high mortality in humans. Antigen-presenting cells, particular dendritic cells (DCs), are early and preferred targets of LASV, their productive infection contributes to the virus-induced immunosuppression observed fatal disease. Here, we characterized role C-type lectin DC-specific ICAM-3-grabbing nonintegrin (DC-SIGN) LASV entry into primary human DCs using chimera prototypic lymphocytic choriomeningitis (LCMV)...
Bunyaviruses represent a growing threat to humans and livestock globally. The receptors, cellular factors endocytic pathways used by these emerging pathogens infect cells remain largely unidentified poorly characterized. DC‐SIGN is C‐type lectin highly expressed on dermal dendritic that has been found act as an authentic entry receptor for many phleboviruses ( Bunyaviridae ), including Rift Valley fever virus RVFV Toscana TOSV ) Uukuniemi UUKV ). We can exploit another lectin, L‐ SIGN ,...
Abstract Amyloid fibrils result from the aggregation of host cell-encoded proteins, many giving rise to specific human illnesses such as Alzheimer’s disease. Here we show that major virulence factor Rift Valley fever virus, protein NSs, forms filamentous structures in brain mice and affects mortality. NSs assembles into nuclear cytosolic disulfide bond-dependent fibrillary aggregates infected cells. structural arrangements exhibit characteristics typical for amyloids, an ultrastructure 12...
ABSTRACT The Bunyaviridae constitute a large family of enveloped animal viruses, many which are important emerging pathogens. How bunyaviruses enter and infect mammalian cells remains largely uncharacterized. We used two genome-wide silencing screens with distinct small interfering RNA (siRNA) libraries to investigate host proteins required during infection human by the bunyavirus Uukuniemi virus (UUKV), late-penetrating virus. Sequence analysis revealed that siRNAs in inhibited not only...
How hantaviruses assemble and exit infected cells remains largely unknown. Here, we show that the expression of Andes (ANDV) Puumala (PUUV) hantavirus Gn Gc envelope glycoproteins lead to their self-assembly into virus-like particles (VLPs) which were released cell supernatants. The viral nucleoprotein was not required for particle formation. Further, a endodomain deletion mutant did abrogate VLP VLPs pleomorphic, exposed protrusions reacted with patient sera.
Abstract Hexosylceramides (HexCer) are implicated in the infection process of various pathogens. However, molecular and cellular functions HexCer infectious cycles poorly understood. Investigating enveloped virus Uukuniemi (UUKV), a bunyavirus Phenuiviridae family, we performed lipidomic analysis with mass spectrometry determined lipidome both infected cells derived virions. We found that UUKV alters processing to glycosphingolipids (GSL) cells. The resulted overexpression glucosylceramide...
The impact of hepatitis C virus NS5A protein mutations on interferon alfa (IFN-alpha) signaling pathway, cell proliferation, and viability is an important issue that still under debate. We have therefore combined transient stable expression in a human hepatocytic line (Huh7) 3 full-length sequences, isolated from patients with or without response to IFN-alpha therapy. Expression all NS5A-reduced global antiviral activity both vesicular stomatitis (VSV) encephalomyocarditis (EMCV)...
ABSTRACT In the last decade, novel tick-borne pathogenic phleboviruses in family Bunyaviridae , all closely related to Uukuniemi virus (UUKV), have emerged on different continents. To reproduce tick-mammal switch vitro we first established a reverse genetics system rescue UUKV with genome close that of authentic isolated from Ixodes ricinus tick reservoir. The IRE/CTVM19 and IRE/CTVM20 cell lines, both derived I. were susceptible rescued plasmid DNAs supported production over many weeks,...