A5029709232- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Vector-borne infectious diseases
- interferon and immune responses
- Vector-Borne Animal Diseases
- COVID-19 Clinical Research Studies
- Viral Infections and Outbreaks Research
- SARS-CoV-2 and COVID-19 Research
- Plant Virus Research Studies
- COVID-19 Impact on Reproduction
- Fire effects on ecosystems
- RNA and protein synthesis mechanisms
- Malaria Research and Control
- vaccines and immunoinformatics approaches
- RNA modifications and cancer
- Cell Image Analysis Techniques
- Bacteriophages and microbial interactions
- RNA regulation and disease
- COVID-19 epidemiological studies
- COVID-19 and healthcare impacts
- SARS-CoV-2 detection and testing
- Advanced Fluorescence Microscopy Techniques
- Endoplasmic Reticulum Stress and Disease
- Cytomegalovirus and herpesvirus research
- Monoclonal and Polyclonal Antibodies Research
Umeå University
2016-2025
Universität der Bundeswehr München
2018
Charles River Laboratories (Germany)
2018
Helmholtz Centre for Infection Research
2018
Otto-von-Guericke University Magdeburg
2018
Public Health Agency of Sweden
2018
Institut für Mikrobiologie der Bundeswehr
2018
National Cheng Kung University
2018
University of Freiburg
2008-2013
Institute of Medical Microbiology and Hygiene
2010
Circulating monocytes can compete for virtually any tissue macrophage niche and become long-lived replacements that are phenotypically indistinguishable from their embryonic counterparts. As the factors regulating this process incompletely understood, we studied competition in brain by depleting microglia with >95% efficiency using Cx3cr1CreER/+R26DTA/+ mice monitored long-term repopulation. Here show microglial is repopulated within weeks a combination of local proliferation...
Rift Valley fever virus (RVFV) continues to cause large outbreaks of acute febrile and often fatal illness among humans domesticated animals in Africa, Saudi Arabia, Yemen. The high pathogenicity this bunyavirus is mainly due the viral protein NSs, which was shown prevent transcriptional induction antivirally active type I interferons (alpha/beta interferon [IFN-alpha/beta]). Viruses lacking NSs gene induce synthesis IFNs are therefore attenuated, whereas noninducing wild-type RVFV strains...
ABSTRACT Tick-borne encephalitis virus (TBEV) (family Flaviviridae , genus Flavivirus ) accounts for approximately 10,000 annual cases of severe in Europe and Asia. Here, we investigated the induction antiviral type I interferons (IFNs) (alpha/beta IFN [IFN-α/β]) by TBEV. Using strains Neudörfl, Hypr, Absettarov, demonstrate that levels IFN-β transcripts viral RNA are strictly correlated. Moreover, TBEV was dependent on transcription factor regulatory 3 (IRF-3). However, even strain which...
ABSTRACT Rift Valley fever virus (RVFV) is a member of the genus Phlebovirus within family Bunyaviridae . It mosquito-borne zoonotic agent that can cause hemorrhagic in humans. The enveloped RVFV virions are known to be covered by capsomers glycoproteins G N and C , organized on T=12 icosahedral lattice. However, structural units forming have not been determined. Conflicting biochemical results for another phlebovirus (Uukuniemi virus) indicated existence either homodimers or -G heterodimers...
Neurotropic flaviviruses such as tick-borne encephalitis virus (TBEV), Japanese (JEV), West Nile (WNV), and Zika (ZIKV) are causative agents of severe brain-related diseases including meningitis, encephalitis, microcephaly. We have previously shown that local type I interferon response within the central nervous system (CNS) is involved in protection mice against flavivirus infection. However, cells responsible for mounting this protective not defined.Primary astrocytes were isolated from...
Flaviviruses are arthropod-borne viruses that constitute a major global health problem, with millions of human infections annually. Their pathogenesis ranges from mild illness to severe manifestations such as hemorrhagic fever and fatal encephalitis. Type I interferons (IFNs) induced in response viral infection stimulate the expression interferon-stimulated genes (ISGs), including encoding viperin (virus-inhibitory protein, endoplasmic reticulum associated, IFN inducible), which shows...
Viral proteins make extensive use of short peptide interaction motifs to hijack cellular host factors. However, most current large-scale methods do not identify this important class protein-protein interactions. Uncovering mediated interactions provides both a molecular understanding viral with their and the foundation for developing novel antiviral reagents. Here we describe discovery approach covering 23 coronavirus strains that high resolution information on direct virus-host We 269...
Viruses mimic host short linear motifs (SLiMs) to hijack and deregulate cellular functions. Studies of motif-mediated interactions therefore provide insight into virus-host dependencies, reveal targets for therapeutic intervention. Here, we describe the pan-viral discovery 1712 SLiM-based using a phage peptidome tiling intrinsically disordered protein regions 229 RNA viruses. We find mimicry SLiMs be ubiquitous viral strategy, novel proteins hijacked by viruses, identify pathways frequently...
Bunyaviridae is a large family of viruses that have gained attention as "emerging viruses" because many members cause serious disease in humans, with an increasing number outbreaks. These negative-strand RNA possess membrane envelope covered by glycoproteins. The virions are pleiomorphic and thus not been amenable to structural characterization using common techniques involve averaging electron microscopic images. Here, we determined the three-dimensional structure member cryotomography....
Viperin is an interferon-induced protein with a broad antiviral activity. This evolutionary conserved contains radical S-adenosyl-l-methionine (SAM) domain which has been shown in vitro to hold [4Fe-4S] cluster. We identified tick-borne encephalitis virus (TBEV) as novel target for human viperin inhibits productionof the viral genome RNA. Wt was found require ER localization full activity and interact cytosolic Fe/S assembly factor CIAO1. Radiolabelling vivo revealed incorporation of (55)...
La Crosse encephalitis virus (LACV) is a mosquito-borne member of the negative-strand RNA family Bunyaviridae. We have previously shown that virulence factor NSs LACV an efficient inhibitor antiviral type I interferon system. A recombinant unable to express (rLACVdelNSs) strongly induced transcription, whereas corresponding wt (rLACV) suppressed it. Here, we show induction by rLACVdelNSs mainly occurs through signaling pathway leading from pattern recognition receptor RIG-I transcription...
ABSTRACT Vector-borne flaviviruses, such as tick-borne encephalitis virus (TBEV), West Nile virus, and dengue cause millions of infections in humans. TBEV causes a broad range pathological symptoms, ranging from meningitis to severe or even hemorrhagic fever, with high mortality. Despite the availability an effective vaccine, incidence is increasing. Not much known about role innate immune system control infections. Here, we show that type I interferon (IFN) essential for protection against...
Although type I interferons (IFNs)—key effectors of antiviral innate immunity are known to be induced via different pattern recognition receptors (PRRs), the cellular source and relative contribution PRRs in host protection against viral infection is often unclear. IPS-1 a downstream adaptor for retinoid-inducible gene (RIG-I)-like receptor signaling. In this study, we investigate immune response brain regions during with tick-borne encephalitis virus (TBEV), flavivirus that causes variety...
Men are more severely affected by COVID-19. Testosterone may influence SARS-CoV-2 infection and the immune response. To clinically, epidemiologically, experimentally evaluate effect of antiandrogens on infection. A randomized phase 2 clinical trial (COVIDENZA) enrolled 42 hospitalized COVID-19 patients before safety evaluation. We also conducted a population-based retrospective study 7894 SARS-CoV-2–positive prostate cancer an experimental using air-liquid interface three-dimensional culture...
Tick-borne encephalitis virus (TBEV) is a pathogenic, enveloped, positive-stranded RNA in the family Flaviviridae. Structural studies of flavivirus virions have primarily focused on mosquito-borne species, with only one cryo-electron microscopy (cryo-EM) structure tick-borne species published. Here, we present 3.3 Å cryo-EM TBEV virion Kuutsalo-14 isolate, confirming overall organisation virus. We observe conformational switching peripheral and transmembrane helices M protein, which can...
We have analyzed the importance of specific amino acids in cytoplasmic tail glycoprotein G(N) for packaging ribonucleoproteins (RNPs) into virus-like particles (VLPs) Uukuniemi virus (UUK virus), a member Bunyaviridae family. In order to study packaging, we added G(N)/G(C) precursor (p110) polymerase I-driven minigenome rescue system generate VLPs that are released supernatant. These can infect new cells, and reporter gene expression be detected. To determine role UUK glycoproteins RNP...
ABSTRACT Efficient antiviral immunity requires interference with virus replication at multiple layers targeting diverse steps in the viral life cycle. We describe here a novel flavivirus inhibition mechanism that results interferon-mediated obstruction of tick-borne encephalitis particle assembly and involves release malfunctioning membrane-associated capsid (C) particles. This is controlled by activity interferon-induced protein viperin, broad-spectrum interferon-stimulated gene. Through...
Flaviviruses are a group of diverse and emerging arboviruses an immense global health problem. A number flaviviruses neurotropic, causing severe encephalitis even death. Type I interferons (IFNs) the first line defense innate immune system against flavivirus infection. IFNs elicit concerted action numerous interferon-stimulated genes (ISGs) to restrict both virus infection replication. Viperin (virus-inhibitory protein, endoplasmic reticulum-associated, IFN-inducible) is ISG with...
Viral tropism within the brain and role(s) of vertebrate immune response to neurotropic flaviviruses infection is largely understudied. We combine multimodal imaging (cm-nm scale) with single nuclei RNA-sequencing study Langat virus in wildtype interferon alpha/beta receptor knockout (Ifnar
In the present report we describe an infectious virus-like particle (VLP) system for Uukuniemi (UUK) virus, a member of Bunyaviridae family. It utilizes our recently developed reverse genetic based on RNA polymerase I minigenome UUK virus used to study replication, encapsidation, and transcription by monitoring reporter gene expression. Here, have added glycoprotein precursor expression plasmid together with minigenome, nucleoprotein, generate VLPs, which incorporate are released into...
The tick-borne encephalitis virus (TBEV) is a flavivirus transmitted to humans, usually via tick bites. causes (TBE) in and symptoms range from mild flu-like severe long-lasting sequelae, including permanent brain damage. It has been suggested that within the population of viruses mammalian host, quasispecies with neurotropic properties might become dominant host resulting neurological symptoms. We previously demonstrated existence TBEV variants variable poly(A) tracts single blood-fed tick....