A5042699462- Escherichia coli research studies
- Glycosylation and Glycoproteins Research
- Folate and B Vitamins Research
- Carbohydrate Chemistry and Synthesis
- Epigenetics and DNA Methylation
- Gut microbiota and health
- Viral gastroenteritis research and epidemiology
- Clostridium difficile and Clostridium perfringens research
- Vibrio bacteria research studies
- Ubiquitin and proteasome pathways
- Cancer Research and Treatments
- Genomics and Rare Diseases
- Metabolism and Genetic Disorders
- Birth, Development, and Health
- Monoclonal and Polyclonal Antibodies Research
- Sphingolipid Metabolism and Signaling
- Salmonella and Campylobacter epidemiology
- Cytomegalovirus and herpesvirus research
- Microscopic Colitis
- Neuroinflammation and Neurodegeneration Mechanisms
- CRISPR and Genetic Engineering
- Influenza Virus Research Studies
- Probiotics and Fermented Foods
- Vitamin C and Antioxidants Research
- Bioinformatics and Genomic Networks
University of Gothenburg
2017-2021
Wellcome Sanger Institute
2011-2016
Novartis (Italy)
2013
Mutations in whole organisms are powerful ways of interrogating gene function a realistic context. We describe program, the Sanger Institute Mouse Genetics Project, that provides step toward aim knocking out all genes and screening each line for broad range traits. found hitherto unpublished were as likely to reveal phenotypes known genes, suggesting novel represent rich resource investigating molecular basis disease. many unexpected detected only because we screened them, emphasizing value...
Clostridium difficile persists in hospitals by exploiting an infection cycle that is dependent on humans shedding highly resistant and infectious spores. Here we show human virulent C. can asymptomatically colonize the intestines of immunocompetent mice, establishing a carrier state for many months. mice consistently shed low levels spores but, surprisingly, do not transmit to cohabiting mice. However, antibiotic treatment carriers triggers contagious supershedder state, characterized...
Our intestinal microbiota harbors a diverse microbial community, often containing opportunistic bacteria with virulence potential. However, mutualistic host-microbial interactions prevent disease by pathogens through poorly understood mechanisms. We show that the epithelial interleukin-22 receptor IL-22RA1 protects against lethal Citrobacter rodentium infection and chemical-induced colitis promoting colonization resistance an bacterium, Enterococcus faecalis. Susceptibility of Il22ra1−/−...
Both maternal and offspring-derived factors contribute to lifelong growth bone mass accrual, although the specific role of deficiencies in offspring is poorly understood. In present study, we have shown that vitamin B12 (B12) deficiency a murine genetic model results severe postweaning retardation osteoporosis, severity time onset this phenotype depends on genotype. Using integrated physiological metabolomic analysis, determined decreases liver taurine production associates with abrogation...
Abstract Sphingosine-1-phosphate (S1P) is lipid messenger involved in the regulation of embryonic development, immune system functions, and many other physiological processes. However, mechanisms S1P transport across cellular membranes remain poorly understood, with several ATP-binding cassette family members spinster 2 (Spns2) member major facilitator superfamily known to mediate cell culture. Spns2 was also shown control activities zebrafish vivo play a critical role cardiovascular...
Abstract Polymorphisms of the FUT2 gene alters glycan ABO(H) blood group and Lewis antigen expression (commonly known as non-secretor status) in small intestinal mucosa. Whilst status affects 20% population worldwide, it has been reported to be present up 40% all Bangladeshis. Furthermore, Bangladeshi children are reportedly more susceptible symptomatic enterotoxigenic Escherichia coli (ETEC) infection if they non-secretors. Therefore, an attempt identify a genotypic biomarker altered...
A promising strategy to limit cholera severity involves blockers mimicking the canonical toxin ligand (CT) ganglioside GM1. However, date efficacies of most these have been evaluated in noncellular systems that lack ligands other than Importantly, CT B subunit (CTB) has a noncanonical site binds fucosylated structures, which contrast GM1 are highly expressed human intestine. Here we evaluate capacity norbornene polymers displaying galactose and/or fucose block CTB binding immobilized...
Interleukin (IL)-22 is a cytokine that plays critical role in intestinal epithelial homeostasis. Its downstream functions are mediated through interaction with the heterodimeric IL-22 receptor and subsequent activation of signal transducer activator transcription 3 (STAT3). signaling can induce genes necessary for cell proliferation, tissue regeneration, tight junction fortification, antimicrobial production. Recent studies have also implicated regulation fucosylation mice. However, whether...
Proteins exhibiting hyper-variable sequences within a bacterial pathogen may be associated with host adaptation. Several lineages of the monophyletic Salmonella enterica serovar Typhi (S. Typhi) have accumulated non-synonymous mutations in putative two-component regulatory system yehUT. Consequently we evaluated function yehUT S. BRD948 and Typhimurium ST4/74. Transcriptome analysis identified cstA gene, encoding carbon starvation protein as predominantly regulated gene both these serovars....
ABSTRACT Mice harboring a mutation in the gene encoding gastric intrinsic factor (Gif), protein essential for absorption of vitamin B 12 /cobalamin (Cbl), have potential as model to explore role vitamins infection. The levels Cbl blood Gif tm1a/tm1a mutant mice were influenced by maternal genotype, with offspring born heterozygous (high Cbl, F 1 ) mothers exhibiting significantly higher serum level than those homozygous (low 2 equivalents. Low correlated susceptibility an infectious...
Vaccines against enteric diseases could improve global health. Despite this, only a few oral vaccines are currently available for human use. One way to facilitate such vaccine development be identify practical and relatively low cost biomarker assay assess induced primary memory IgA immune responses in humans. Such an complement antigen-specific response measurements, enabling more candidates tested, whilst also reducing the work costs associated with early development. With this mind, we...
We have previously reported clinical data to suggest that colonization factor I (CFA/I) fimbriae of enterotoxigenic Escherichia coli (ETEC) can bind Lewis a (Lea), glycan epitope ubiquitous in the small intestinal mucosa young children (<2 years age), and individuals with genetic mutation FUT2. To further elucidate physiological binding properties this interaction, we engineered Chinese Hamster Ovary (CHO-K1) cells express Lea or Leb determinants on both N- O-glycans. used our...