A5091176143- Proteoglycans and glycosaminoglycans research
- Chemical Synthesis and Analysis
- RNA Interference and Gene Delivery
- Glycosylation and Glycoproteins Research
- Nanoparticle-Based Drug Delivery
- Monoclonal and Polyclonal Antibodies Research
- Advanced Drug Delivery Systems
- Radiopharmaceutical Chemistry and Applications
- Carbohydrate Chemistry and Synthesis
- PARP inhibition in cancer therapy
- DNA Repair Mechanisms
- Immunotherapy and Immune Responses
- Advanced biosensing and bioanalysis techniques
- Click Chemistry and Applications
- Immune cells in cancer
- Innovative Microfluidic and Catalytic Techniques Innovation
- Chemical Reactions and Isotopes
- T-cell and B-cell Immunology
- Electron Spin Resonance Studies
- Dendrimers and Hyperbranched Polymers
- High Entropy Alloys Studies
- Additive Manufacturing Materials and Processes
- Inhalation and Respiratory Drug Delivery
- Characterization and Applications of Magnetic Nanoparticles
- Metal and Thin Film Mechanics
Vinci (United Kingdom)
2025
Italian Institute of Technology
2018-2022
Istituto Nazionale di Fisica Nucleare, Sezione di Genova
2020
University of Manchester
2014-2019
Manchester Academic Health Science Centre
2017-2019
University of Bologna
2009-2010
CD44 is a potentially rewarding target in cancer therapy, although its mechanisms of ligand binding and internalization are still poorly understood. In this study, we have established quantitative relationships between expression differently polarized macrophages (M0, M1, M2‐polarized THP‐1 human macrophages) the uptake hyaluronic acid (HA)‐based materials, which usable for targeting. We validated robust method macrophage polarization, sequentially uses differentiating polarizing factors,...
Chitosan/hyaluronic acid (HA) nanoparticles can be used to deliver an RNA/DNA cargo cells overexpressing HA receptors such as CD44. For these systems, unequivocal links have not been established yet between chitosan macromolecular (molecular weight; degree of deacetylation, i.e., charge density) and nanoparticle variables (complexation strength, stability; nucleic protection; internalization rate) on one hand, transfection efficiency the other hand. Here, we focused role avidity in...
Abstract CD44 is an endocytic hyaluronic acid (HA) receptor, and overexpressed in many carcinomas. This has encouraged the use of HA to design CD44‐targeting carriers. paper about dissecting mechanistic role CD44. Here, HA‐decorated nanoparticles are used deliver siRNA both tumoral (AsPC‐1, PANC‐1, HT‐29, HCT‐116) non‐tumoral (fibroblasts, differently polarized THP‐1 macrophages, HUVEC) human cell lines, evaluating initial binding nanoparticles, their internalization rate, silencing...
Nanoparticles based on hyaluronic acid (HA) are designed to deliver tannic (TA) as an antimicrobial agent. The presence of HA makes these particles potentially useful target bacteria that colonize cells presenting membrane receptors (e.g. CD44), such macrophages. bearing 3‐aminophenyl boronic groups (HA‐APBA) is reacted with TA, yielding nanoparticles a size decreases decreasing molecular weight 200 nm for 44 kDa, 400 737 kDa). boronate esters make the stable at physiological pH, but their...
This study presents a quantitative assessment of the complexation between boronic acids and diols as reversible double-stimulus (oxidation acidification)-responsive bioconjugation reaction. First, by using competition assay, we have evaluated equilibrium constants (water, pH 7.4) 34 boronate/diol pairs, both aliphatic aromatic (catechols) nature; in general, catechols were characterized 3 orders magnitude higher than those diols. Second, demonstrated that successful with generated situ via...
This study addresses well-known shortcomings of poly(ethylene glycol) (PEG)-based conjugates. PEGylation is by far the most common method employed to overcome immunogenicity and suboptimal pharmacokinetics of, for example, therapeutic proteins but has significant drawbacks. First, PEG offers no protection from denaturation during lyophilization, storage, or oxidation (e.g., biological oxidants, reactive oxygen species); second, PEG's inherent immunogenicity, leading hypersensitivity...
This study is about linking preparative processes of nanoparticles with the morphology and their efficiency in delivering payloads intracellularly. The are composed hyaluronic acid (HA) chitosan; former can address a nanoparticle to cell surface receptors such as CD44, second allows both for entrapment nucleic acids an endosomolytic activity that facilitates liberation cytoplasm. Here, we have systematically compared prepared either A) through two-step process based on intermediate...
Keratin based electrospun fibres containing cinnamon essential oil are highly antioxidant and antibacterial, promote reduced tissue inflammation after skin burns.
We present a method for tyrosine-selective and reversible bioconjugation; tyrosines are enzymatically converted into catechols in situ "clicked" onto boronic acids. Importantly, our process selectively produces avoids quinones, thereby improving the control over chemical identity of products. have conjugated acid-containing hyaluronic acid (HyA) to peptides bearing variable number position; use tagging provision well exposed tyrosine residues—in case hemagglutinin-derived HA-tag—makes...
The selective targeting of dendritic cells (DCs) can lead to more efficacious vaccines. Here, materials have been designed for a synergic DC targeting: interacting with CD44 through the use hyaluronic acid (HA), and mannose‐binding lectins (typical pattern recognition receptors) HA mannosylation. Negatively charged, HA‐displaying nanoparticles are produced via polyelectrolyte complexation (mannosylated) high‐ or low‐ molecular‐weight chitosan (CS, 36 656 kDa). Using CS36, is better exposed...
We show the first example of a synergic approach oxidant (ROS) scavenging carrier and ROS-responsive drug release in context potential therapy against osteoporosis, aiming to inhibit differentiation inflammatory cells into osteoclasts. In our "tandem" approach, branched amphiphilic, PEGylated polysulfide (PPSES-PEG) was preferred over linear analogue, because improved homogeneity aggregates (spherical micelles vs mixture wormlike spherical), increased stability, higher loading (up ∼22 wt %...
Families of amphiphilic oxidation-responsive polymers (poly(ethylene glycol)-polysulfides) with different architectures (linear, 4, 6, and 8-armed stars 10, 15, 20-armed combs) compositions (variable ethylene sulfide/propylene sulfide ratio) are prepared. In water, all the assemble in spherical micelles, critical micellar concentrations <0.01 mg mL-1 for branched polymers. Triple-detection gel permeation chromatography (GPC) asymmetric field flow fractionation (AFFF) dynamic static light...
Hybrids composed of liposomes (L) and metallic nanoparticles (NPs) hold great potential for imaging drug delivery purposes. However, the efficient incorporation NPs into using conventional methodologies has so far proved to be challenging. In this study, we report fabrication hybrids hydrophobic gold size 2–4 nm (Au) a microfluidic-assisted self-assembly process. The increasing amounts AuNPs was examined microfluidics compared L–AuNP prepared by reverse-phase evaporation method. Our strategy...
The cytotoxic action of anticancer drugs can be potentiated by inhibiting DNA repair mechanisms. RAD51 is a crucial protein for genomic stability due to its critical role in the homologous recombination (HR) pathway. BRCA2 assists fibrillation and defibrillation cytoplasm nucleus nuclear transport. BRC4 peptide derived from fourth BRC repeat BRCA2, it lacks localization sequence. Here, we used (i) reverse fibrillation; (ii) avoid transport RAD51; (iii) inhibit HR enhance efficacy...
Chemokine-induced chemotaxis mediates physiological and pathological immune cell trafficking, as well several processes involving migration. Among them, the role of CXCL12/CXCR4 signaling in cancer metastasis is known, CXCR4 has been often targeted with small molecule-antagonists or short CXCL12-derived peptides to limit migration invasion. To reduce CXCR4-mediated chemotaxis, we adopted a different approach. We manufactured poly(lactic acid-co-glycolic acid) (PLGA)/Pluronic F127...
Hyaluronic acid (HA)-based prodrugs bearing double-responsive (acid pH or oxidation) boronates of catechol-containing drugs were used to treat xenografted human prostate tumours (LNCaP) in SCID mice. The HA accumulated significantly only (impressively, up 40% the injected dose after 24 h) and liver, with negligible - actually anti-inflammatory consequences latter. A quercetin-HA prodrug slowed down tumour growth, a dose-dependent fashion much higher efficacy (up 4 times) than equivalent...
We describe how the organocatalytic, 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU)-based lactide ring-opening polymerization can be effectively performed in a very polar solvent, N-methylpyrrolidone (NMP). Due to low ceiling temperature, this "living" mechanism has been unreported date, but we here demonstrate that through combination of temperature and repeated monomer additions (starve-fed process), enables generation plethora multifunctional homo- (stereo)block-poly(lactide)s (PLAs) with...
This study is about (1) nanomanufacturing (focusing on microfluidic-assisted nanoprecipitation), (2) advanced colloid characterization field flow fractionation), and (3) the possible restructuring of surface disulfides. Disulfides are dynamic exchangeable groups, here we specifically focus, first, their use to introduce biofunctional groups and, second, re-organization, which may lead variable chemistries uncontrolled cell interactions. The particles were obtained via (flow-focused)...
Abstract In the course of a program devoted to synthesis small peptidic molecules mimicking RGD (Arg‐Gly‐Asp) motif, allylic amination enantiomerically pure carbonates with 4‐substituted benzylamines afforded dehydro‐β‐amino esters through an S N 2′ mechanism. The reaction performed 4‐aminobenzylamine occurred complete chemoselectivity, as aliphatic amine was much more reactive than aromatic one. This allowed useful precursors biologically active compounds be obtained, avoiding extra...
A nano MRI contrast agent composed of molecular magnets loaded in polysulfide nanoparticles senses <0.1 mM hydrogen peroxide.