A5031407235- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Antibiotic Resistance in Bacteria
- Carcinogens and Genotoxicity Assessment
- Microtubule and mitosis dynamics
- Cancer Genomics and Diagnostics
- Science, Research, and Medicine
- Asthma and respiratory diseases
- Polyomavirus and related diseases
- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- Malaria Research and Control
- Drug-Induced Hepatotoxicity and Protection
Massachusetts Institute of Technology
2015-2024
Agency for Science, Technology and Research
2016-2020
Institute of Molecular and Cell Biology
2020
Center for Environmental Health
2020
Streptococcus pneumoniae is a leading cause of pneumonia and one the most common causes death globally. The impact S. on host molecular processes that lead to detrimental pulmonary consequences not fully understood. Here, we show induces toxic DNA double-strand breaks (DSBs) in human alveolar epithelial cells, as indicated by ataxia telangiectasia mutated kinase (ATM)-dependent phosphorylation histone H2AX colocalization with p53-binding protein (53BP1). Furthermore, results damage occurs...
Southeast Asia has been the hotbed for development of drug-resistant malaria parasites, including those with resistance to artemisinin combination therapy. While mutations in kelch propeller domain (K13 mutations) are associated resistance, a range evidence suggests that other factors critical establishment and subsequent transmission field. Here, we perform quantitative analysis DNA damage repair parasite Plasmodium falciparum find strong link between enhanced resistance. This experimental...
Dysfunction of apoptosis and DNA damage response pathways often drive cancer, so a better understanding these can contribute to new cancer therapeutic strategies. Diverse discovery approaches have identified many regulators, response, repair proteins; however, rely on indirect detection damage. Here, we describe novel platform based the comet assay that leverages previous technical advances in precision by incorporating high-throughput robotics. The screening (HTS) CometChip is first...
ABSTRACT DNA damage can be cytotoxic and mutagenic is directly linked to aging, cancer, heritable diseases. To counteract the deleterious effects of damage, cells have evolved highly conserved repair pathways. Many commonly used assays are relatively low throughput limited analysis one protein or pathway. Here, we explored capacity CometChip platform for parallel multiple activities. Taking advantage versatility traditional comet assay leveraging micropatterning techniques, offers increased...