Flaxseed oil cake (FOC) was evaluated as a potential substrate for the production of novel kefir-like fermented beverage. Three variants containing 5%, 10%, and 15% (w/w) FOC were inoculated with kefir grains incubated at 25 °C 24 h. After processing, beverages stored in refrigerated conditions (6 °C) 21 days. Changes microbial population, pH, acidity, levels proteins, polyphenolics, flavonoids, ascorbic acid, reducing sugars estimated. Additionally, viscosity, firmness, color, antioxidant...

Rationale: The objective of this autopsy study was to determine whether gastrointestinal angiodysplasia develops during continuous-flow left ventricular assist device (LVAD) support. Objective: LVAD support causes pathologic degradation von Willebrand factor (vWF) and bleeding from at an alarming rate. It has been speculated that itself may cause angiodysplasia. relationship abnormal vWF metabolism is unknown. We tested the hypothesis vascularity Methods Results: Small bowel obtained...

In the post-antibiotic era issue of bacterial resistance refers not only to antibiotics themselves but also common antiseptics like octenidine dihydrochloride (OCT). This appears as an emerging challenge in terms preventing staphylococcal infections, which are both potentially severe and easy transfer horizontally. Essential oils have shown synergisms with antiseptics. Therefore aim this study was investigate impact lavender essential oil (LEO) on OCT efficiency towards methicillin-resistant...

It is well known that ion implantation one of the basic tools for semiconductor device fabrication. The process itself damages, however, crystallographic lattice semiconductor. Such damage can be removed by proper post-implantation annealing implanted material. Annealing also allows electrical activation dopant and creates areas different types in a However, such thermal treatment particularly challenging case gallium nitride since it decomposes at relatively low temperature (~800 °C)...

Nonhuman primates (NHPs) have been widely used in different porcine xenograft procedures inevitably resulting exposure to endogenous retrovirus (PERV). Surveillance for PERV infection these NHPs may provide information on the risks of cross-species transmission PERV, particularly recipients vascularized organ xenografts whom data from human clinical trials is unavailable.We tested 21 Old World and 2 New exposed a variety evidence infection. These included six baboon pig hearts, bonnet...

During cardiopulmonary bypass, global hypoperfusion of the brain has been shown to result in ischemic insult and subsequent neurologic injury. Furthermore, outcome after focal cerebral ischemia depends on collateral circulation, which is determined by parameters perfusion. We therefore measured blood flow during independent manipulations arterial pressure pump rate determine these hemodynamic regulates perfusion bypass.Seven anesthesized baboons were placed bypass cooled 28 degrees C. Pump...

Preparation of biodegradable packaging materials and valorisation food industry residues to achieve “zero waste” goals is still a major challenge. Herein, biopolymer-based (carboxymethyl cellulose—CMC) bioactive films were prepared by the addition, alone or in combination, carvacrol fungal melanin isolated from champignon mushroom (Agaricus bisporus) agro-industrial residues. The mechanical, optical, thermal, water vapour, UV-Vis barrier properties studied. Fourier-transform infrared (FT-IR)...

The World Health Organization points out that the opportunistic pathogen Klebsiella pneumoniae causes various infections among others, urinary tract (UTIs), is one of high-priority species due to a global problem antimicrobial resistance. aim this study was investigate antibacterial and anti-biofilm activities chosen constituents essential oils against NDM-1-producing, uropathogenic K. strains. genes encoding lipopolysaccharide (uge, wabG), adhesin gene fimH (type I fimbriae) carbapenemase...

The shortage of organs for transplantation is especially severe the critically ill newborn infant, whom donors appropriate size are particularly scarce. One way to overcome this problem use animals in lieu humans as organ donors. major limitation using purpose susceptibility animal hyperacute rejection, a violent rejection reaction thought be mediated by antidonor antibody and complement. To evaluate potential application xenotransplantation newborns, we tested neonatal baboons found that...

It is unclear whether oxygen plays a role in stem cell therapy. Hence, the determination of local oxygenation (Po(2)) infarct heart and at site transplantation may be critical to study efficacy To demonstrate this, we have developed an oxygen-sensing paramagnetic spin probes (OxySpin) monitor region using electron resonance (EPR) spectroscopy. Skeletal myoblast (SM) cells isolated from thigh muscle biopsies mice were labeled with OxySpin by coculturing submicron-sized (270 +/- 120 nm)...

This study aimed to determine the effect of selected essential oil compounds (EOCs) on antibacterial activity β-lactam antibiotics (βLAs) against methicillin-resistant Staphylococcus aureus (MRSA) strains. The following parameters were studied: antibiotic susceptibility testing, detection mecA gene and evaluation genotypic relativity isolates using molecular techniques, analysis chemical composition applying Fourier-transform infrared (FTIR) spectroscopy, determination EOCs alone in...

Plant derived fermented beverages have recently gained consumers’ interest, particularly due to their intrinsic functional properties and presence of beneficial microorganisms. Three variants containing 5%, 10%, 15% (w/w) sweet blue lupin (Lupinus angustifolius L. cv. “Boregine”) seeds were inoculated with kefir grains incubated at 25 °C for 24 h. After processing, stored in refrigerated conditions (6 °C) 21 days. Changes microbial population, pH, bioactive compounds (polyphenolics,...

Currently, serological tests for Lyme disease (LD), routinely performed in laboratories following the European Concerted Action on Borreliosis recommendations as part of two-stage diagnostics, are often difficult to interpret. This concerns both generation false positive and negative results, which frequently delay correct diagnosis implementation appropriate treatment. The above problems result from morphological antigenic variability characteristics life strategy spirochete Borrelia...

The COVID-19 pandemic made the medical community realize how large a problem it would face. epidemiological situation forced opening of additional wards, so-called “COVID wards”, where an increase in rate coexisting bacterial infections was observed. We report hospital outbreak due to New Delhi carbapenemases producing K. pneumoniae clones. Twenty-eight strains were analyzed from patients with primary infection. drug susceptibility determined by diffusion–circulation method and E-test....

Essential oils (EOs) and their compounds are becoming a growing interest in medical sciences. Despite potential as antimicrobial, anxiolytic, cytotoxic, immunosuppressive drugs, chemical characteristics make them difficult to use direct treatment. This article intends summarize the current body of knowledge regarding drug delivery systems that can overcome obstacles, such low water solubility, volatility, oxidation potential, photodegradation, thermal instability EO compounds. Various...

This study investigates the essential oil (EO) isolated from seeds and cones of Canadian hemlock (Tsuga canadensis), highlighting notable differences in their chemical composition biological activities. The seed EO was uniquely dominated by oxygenated derivatives monoterpene hydrocarbons, particularly bornyl acetate (40%), whereas cone exhibited higher levels hydrocarbons such as α-pinene (23%), β-pinene (20%), myrcene (23%). A significant finding strong cytotoxic activity against melanoma...

We have previously demonstrated that hyperacute rejection does not occur in a pig-to-newborn baboon heart transplant model, presumably because of low levels cytotoxic antipig antibodies present the serum newborn baboons. Cytotoxic are primarily directed to α-1,3-galactosyl (α Gal) residues on endothelial cell surface structures Twenty-one full-term humans and 5 baboons were tested for complement mediated lysis (CML) pig kidney (PK-15) cells anti-α Gal activity with an ELISA using...

Susceptibility of porcine endothelial cells to human natural killer (NK) cell lysis was found reflect surface expression ligands containing Gal α(1,3)GlcNAc, the principal antigen on endothelium recognized by xenoreactive antibodies. Genetically modifying this epitope transfection with α(1,2)-fucosyltransferase gene reduced susceptibility NK lysis. These results indicate that carbohydrate remodeling profoundly affects target lysis, and suggest successful transgenic strategies limit xenograft...

The interaction of T cell costimulatory molecules with their ligands is required for optimal activation. Interference such interactions can induce antigen unresponsiveness and delay xeno- allograft rejection. We have previously shown that LFA3TIP, a soluble human lymphocyte function-associated (LFA)-3 construct, binds CD2 inhibits responses in vitro. This study reports the first use fusion protein, to significantly prolong primate cardiac survival. Based on our observation LFA3TIP baboon...

Summary The present study investigated the effect of different dietary levels DL–methionine (DLM) and MHA-Ca on broiler chicken performance, carcass characteristics meat quality. A total 891 day-old male Ross 308 chickens were allocated to nine groups with 11 replicates each, fed diets supplemented four methionine (0.04, 0.08, 0.16, 0.24%) two sources (DLM MHA-Ca). negative control (NC) diet did not contain any supplemental methionine.

Staphylococcus aureus constitutes the most pathogenic species within staphylococcal genus. Humans remain major reservoirs of this pathogen which colonizes mostly anterior nares healthy individuals.To investigate effect fennel essential oil (FEO) and trans-anethole (tA) on antibacterial activity mupirocin (MUP) against S. strains isolated from asymptomatic carriers.The content FEO was analysed with use GC-MS method. The research done 43 isolates different resistance patterns, obtained nasal...