A5036909791- Bone Tissue Engineering Materials
- Extracellular vesicles in disease
- Phosphodiesterase function and regulation
- MicroRNA in disease regulation
- Mitochondrial Function and Pathology
- Orthopaedic implants and arthroplasty
- RNA Research and Splicing
- Molecular Biology Techniques and Applications
- Spinal Cord Injury Research
- Heart Failure Treatment and Management
- Genetic and Kidney Cyst Diseases
- Fetal and Pediatric Neurological Disorders
- RNA modifications and cancer
- Connexins and lens biology
- Animal Genetics and Reproduction
- Dental Implant Techniques and Outcomes
- Metabolomics and Mass Spectrometry Studies
- Cerebrospinal fluid and hydrocephalus
- Adipose Tissue and Metabolism
- Silk-based biomaterials and applications
- Genetics, Aging, and Longevity in Model Organisms
- Bone and Dental Protein Studies
- Liver physiology and pathology
- Graphene and Nanomaterials Applications
- Hematopoietic Stem Cell Transplantation
Institute of Cell Biology and Neurobiology
2017-2025
Children's Hospital of Philadelphia
2025
National Research Council
2017-2024
European Molecular Biology Laboratory
2024
National Academies of Sciences, Engineering, and Medicine
2008
University of Chieti-Pescara
2007
Bone regeneration represents still a challenge, in particular for calvarium defects. Recently, the development of biomaterials with addiction stem cells is giving promising results treatment bone In particular, it was demonstrated that scaffolds enriched mesenchymal (MSCs) and/or their derivatives, such as conditioned medium (CM) and extracellular vesicles (EVs), may improve regeneration. Moreover, given deep link between osteogenesis angiogenesis, successful approach must also take into...
Bone tissue regeneration strategies require approaches that provide an osteogenic and angiogenic microenvironment able to drive the bone growth. Recently, development of 3D printing biomaterials, including poly(lactide) (3D-PLA), enriched with mesenchymal stem cells (MSCs) and/or their derivatives, such as extracellular vesicles (EVs) has been achieving promising results. In this study, in vitro results showed increased expression markers, RUNX2, VEGFA, OPN COL1A1 living construct...
Abstract Background Myotonic dystrophy type 1 (DM1) is a genetic multisystemic disease, characterised by pleiotropic symptoms that exhibit notable variability in severity, nature and age of onset. The cause DM1 the expansion unstable CTG‐repeats 3′ untranslated region (UTR) DMPK gene, resulting accumulation toxic CUG‐transcripts sequester RNA‐binding proteins form nuclear foci affected tissues and, consequently, alter various cellular processes. Therapeutic gene editing for treatment...
Bone tissue engineering is based on bone grafting to repair defects. graft substitutes can contribute the addition of mesenchymal stem cells (MSCs) in order enhance rate and quality defect regeneration. The cell secretome contains many growth factors chemokines, which could affect cellular characteristics behavior. Conditioned medium (CM) be used regeneration avoiding several problems linked direct use MSCs. In this study, we investigated effect human periodontal ligament (hPDLSCs) their CM...
Background: The process of osseointegration dental implants is characterized by healing phenomena at the level interface between surface and bone. Implant modification has been introduced in order to increase osseointegration. purpose this study evaluate influence biofunctional coatings for bone response a rabbit model. implant coated with collagen type I was analyzed through X-ray Photoelectron Spectroscopy (XPS), Atomic Force Microscopy (AFM), micro-CT histologically. Methods: sandblasted...
Background—the graphene-doping procedure represents a useful to improve the mechanical, physical and biological response of several Polymethyl methacrylate (PMMA)-derived polymers biomaterials for dental applications. The aim this study was evaluate osseointegration Graphene doped Poly(methyl methacrylate) (GD-PMMA) compared with PMMA as potential materials implant devices. Methods—eighteen adult New Zealand white male rabbits mean weight approx. 3000 g were used in research. A total...
ABSTRACT Primary ciliary dyskinesia (PCD) is a genetically heterogeneous disorder affecting normal structure and function of motile cilia, phenotypically manifested as chronic respiratory infections, laterality defects infertility. Autosomal recessive mutations in genes encoding for different components the axoneme have been associated with PCD humans model organisms. The CCDC151 gene encodes coiled-coil axonemal protein that ensures correct attachment outer dynein arm (ODA) complexes to...
Abstract Phosphodiesterase 2A (Pde2A) is a dual-specific PDE that breaks down both cAMP and cGMP cyclic nucleotides. We recently highlighted direct relationship between Pde2A impairment, consequent increase of cAMP, the appearance mouse congenital heart defects (CHDs). Here we aimed to characterize pathways involved in development CHDs their prevention by pharmacological approaches targeting signaling. Transcriptome analysis revealed modulation more than 500 genes affecting biological...
10028 Background: Chimeric antigen receptor T cell (CAR) therapy has revolutionized the treatment of relapsed/refractory pediatric B malignancies. However, financial toxicity (FT) experienced by families is unknown despite likely high burden imposed clinic visits, hospital admissions, and potential travel/relocation to referral centers. We present first report FT among children receiving CAR including prevalence, trajectory, impact on outcomes. Methods: This a prospective cohort analysis...
Botulinum neurotoxin type A (BoNT/A) is a major therapeutic agent that has been proven to be successful treatment for different neurological disorders, with emerging novel indications each year. BoNT/A exerts its action by blocking SNARE complex formation and vesicle release through the specific cleavage of SNAP-25 protein; toxin able block pro-inflammatory molecules months after administration. Here we demonstrate extraordinary capacity neutralize complete paralysis pain insensitivity...
In this work, we applied three-dimensional microCT imaging to study murine embryogenesis in the range from immediate post-implantation period (embryonic day 5.5) mid-gestation 12.5) with resolution up 1.4 µm/voxel. Also, introduce an procedure for non-invasive volumetric estimation of entire litter embryos within maternal uterine structures. This method allows accurate, detailed and systematic morphometric analysis both embryonic extra-embryonic components during embryogenesis....
Odad3 gene loss-of-function mutation leads to Primary Ciliary Dyskinesia (PCD), a disease caused by motile cilia dysfunction. Previously, we demonstrated that knockout of the in mice replicates several features PCD, such as hydrocephalus, defects left–right body symmetry, and male infertility, with complete absence sperm reproductive tract. The majority animals die before sexual maturation due severe hydrocephalus failure thrive, which precludes fertility studies. Here, performed expression...
Abstract Traumatic spinal cord injury has dramatic consequences and a huge social impact. We propose new mouse model of trauma that induces complete paralysis hindlimbs, still observable 30 days after injury. The contusion, performed without laminectomy deriving from the pressure exerted directly on bone, mimics more closely many features in humans. Spinal was injured at thoracic level 10 (T10) adult anesthetized female CD1 mice, mounted stereotaxic apparatus connected to precision impactor...
Phosphodiesterase 2A (PDE2A) is a cAMP-cGMP hydrolyzing enzyme essential for mouse development and the PDE2A knockout model (PDE2A−/−) embryonic lethal. Notably, livers of PDE2A−/− embryos at day 14.5 (E14.5) have extremely reduced size. Morphological, cellular molecular analyses revealed loss integrity in liver niche that compromises hematopoietic function maturation. Hematopoietic cells isolated from are instead able to differentiate vitro assays, suggesting absence blood cell-autonomous...
Glioblastoma (GBM), the most malignant primary brain tumor in adults. Although not frequent, it has a relevant social impact because peak incidence coincides with age of professional maturity. A number novel treatments have been proposed, yet clinical trials disappointing. Recently, phase II trial (REGOMA) demonstrated that multikinase inhibitor regorafenib significantly increased median overall survival (OS) GBM patients when compared to lomustine-treated patients. On this basis, National...
Autism Spectrum Disorder (ASD) is a pervasive neurodevelopmental disorder emerging in early life characterized by impairments social interaction, poor verbal and non-verbal communication, repetitive patterns of behaviors. Among the best-known genetic risk factors for ASD, there are mutations causing loss Fragile X Messenger Ribonucleoprotein 1 (FMRP) leading to syndrome (FXS), common form inherited intellectual disability monogenic cause ASD. Being pivotal regulator motor activity,...
In this study, we used B16-F10 cells grown in the dorsal skinfold chamber (DSC) preparation that allowed us to gain optical access processes triggered by photodynamic therapy (PDT). Partial irradiation of a photosensitized melanoma cell death non-irradiated tumor cells. Multiphoton intravital microscopy with genetically encoded fluorescence indicators revealed bystander was mediated paracrine signaling due adenosine triphosphate (ATP) release from connexin (Cx) hemichannels (HCs)....
Type-specific persistent infection with Human Papillomavirus (HPV) is a significant risk factor for the development of cervical diseases. Persistent could be further refined by sequencing approach to detect early lesions that are at high developing an invasive squamous cancer. The aim present study investigate clinical utility detecting mRNA transcripts HPV oncogenes E6/E7 using Real-time NASBA technology (mRNA test) and identify women low-grade cytological disease but increased high-grade...
Various surface treatments have been tested for titanium implants aiming at increasing their biocompatibility and biological characteristics, but also the efficiency of implant will to be improved drastically decrease peri-implantite mucosite. In fact, peri-implantitis peri-implant mucositis a high incidence in clinical practice. The nanofabrication techniques that offer possibility achieve reduces bacterial colonization could influence osteointegration. aim this research was evaluate bone...
Acquisition of detailed anatomical and molecular knowledge from intact biological samples while preserving their native three-dimensional structure is still a challenging issue for imaging studies aiming to unravel system’s functions. Three-dimensional micro-CT X-ray with high spatial resolution in minimally perturbed naive non-transparent has recently gained increased popularity broad application biomedical research. Here, we describe novel X-ray-based methodology analysis β-galactosidase...
Abstract Study question Has Luteinizing Hormone (LH) a role in the activation/survival of follicles primary ovarian reserve? Summary answer LH promotes survival and perhaps activation/growth primordial follicles, proliferation granulosa cells secondary reduces follicle atresia at later growing stages. What is known already In mammalian ovary, Primordial Follicles (PMFs) constitutes reserve, which declines over time due to physiological events such as atresia, recruitment, ovulation. While...
The oviduct plays a crucial role in the reproductive process, serving as stage for fertilization and early stages of embryonic development. When environment this organ has been mimicked, it shown to enhance vitro embryo epigenetic reprogramming improve yield system. This study explores anatomical intricacies two regions, uterotubal junction (UTJ) ampullary-isthmic (AIJ) by using micro-computed tomography (MicroCT). In study, we have characterized 3D-reconstructed structure, measuring height...