A5017315290- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Glioma Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Medical Imaging Techniques and Applications
- RNA modifications and cancer
- Cell Image Analysis Techniques
- Advanced Electron Microscopy Techniques and Applications
- Data Quality and Management
- Autophagy in Disease and Therapy
- MicroRNA in disease regulation
- Nanoplatforms for cancer theranostics
- Cancer Research and Treatments
- bioluminescence and chemiluminescence research
- Advanced MRI Techniques and Applications
- Electrospun Nanofibers in Biomedical Applications
- Circular RNAs in diseases
- Cancer Immunotherapy and Biomarkers
- Taxation and Compliance Studies
- RNA Research and Splicing
- Immune cells in cancer
- CAR-T cell therapy research
- Labor market dynamics and wage inequality
- Data Visualization and Analytics
University of Milan
2014-2025
University of Florence
2012-2024
Nerviano Medical Sciences
2016
Fondazione Filarete
2015
European School of Molecular Medicine
2015
National Research Council
2015
University of Bologna
2010
Ospedale San Paolo
2007
Bicêtre Hospital
2007
Tumor microenvironment is fundamental for cancer progression and chemoresistance. Among stromal cells tumor-associated macrophages (TAMs) represent the largest population of infiltrating inflammatory in malignant tumors, promoting their growth, invasion immune evasion. M2-polarized TAMs are endowed with NO generating enzyme iNOS. has divergent effects on since it can either stimulate tumor growth or promote death depending source it; likewise role iNOS differs cell type. The generated by TAM...
Glioblastoma multiforme (GBM) is the most aggressive primary brain tumor with poor survival. Cytoreduction in association radiotherapy and temozolomide (TMZ) standard therapy, but response heterogeneous life expectancy limited. The combined use of chemotherapeutic agents drugs targeting cell metabolism becoming an interesting therapeutic option for cancer treatment. Here, we found that metformin (MET) enhances TMZ effect on TMZ-sensitive line (U251) overcomes TMZ-resistance T98G GBM line. In...
Rationale: A common driver of several pathways involved in glioblastoma (GBM) aggressiveness has been recognized the activity HIF-1α transcription factor whose reduction, soon after TMZ administration, furtherly proposed as an early response biomarker GBM models. Since is a tightly regulated protein, study mechanisms its down-regulation could shed new light to explain underpinning sensitivity or resistance TMZ. Methods: The influence silencing cell responsiveness assessed four genetically...
Abstract Interaction between tumor cells and the microenvironment is key in initiation, progression, invasiveness of cancer. In particular, mesenchymal stem (MSCs) are recruited to sites developing tumors, thus promoting metastasis formation. Although it well known that MSCs migrate integrate (TME), their fate function inside still not clear. this study, we analyzed role played by breast cancer oncogenesis. Data indicate interaction with results an increased proliferation metabolic activity...
Glioblastoma (GBM) is the most common astrocytic-derived brain tumor in adults, characterized by a poor prognosis mainly due to resistance available therapy. The study of mitochondria-derived oxidative stress, and biological events that orbit around it, might help comprehension molecular mechanisms at base GBM responsiveness Temozolomide (TMZ). Sensitive resistant cells were used test role mitochondrial ROS release TMZ-resistance. Chaperone-Mediated Autophagy (CMA) activation relation...
Modern medicine relies on a small number of key biologics, which can be found in nature but require further characterization and purification before they used.Since the herbal remedy is given through dated ineffective method drug administration, its effectiveness diminished.The novel form delivery has potential to increase substances while decreasing their side effects.This main idea behind utilising different ways treatments.Several benefits arise from formulations compounds as compared...
The V-ATPase proton pump controls acidification of intra and extra-cellular milieu in both physiological pathological conditions. We previously showed that some subunits are enriched glioma stem cells patients with poor survival. In this study, we investigated how expression a GBM-like influences the non-neoplastic brain microenvironment.Large oncosome (LO) vesicles were isolated from primary glioblastoma (GBM) neurospheres, or patient sera, co-cultured neoplastic cells. LO transcript...
Introduction Dendritic cells play a key role as initiators of T-cell responses, and even if tumour antigen-loaded dendritic can induce anti-tumour their efficacy has been questioned, suggesting need to enhance immunization strategies. Matherials & Methods We focused on the characterization bone marrow-derived pulsed with whole lysate (TAA-DC), source known unknown antigens, in mouse model breast cancer (MMTV-Ras). were evaluated for antigen uptake expression MHC class I/II costimulatory...
We demonstrated that Hsa-miR-567 expression is significantly downregulated in poor prognosis breast cancer, compared to better having a role the control of cell proliferation and migration by regulating KPNA4 gene. In this study, based on our previously published silico results, we proved both vitro (cell line studies) ex vivo (clinical studies), cancer with when prognosis. More intriguingly, ectopic strongly inhibits migration. Furthermore, showed cells, stably expressing Hsa-miR-567,...
Glioblastoma (GBM) is a lethal brain tumor characterized by the glioma stem cell (GSC) niche. The V-ATPase proton pump has been described as crucial factor in sustaining GSC viability and tumorigenicity. Here we studied how patients-derived GSCs rely on activity to sustain mitochondrial bioenergetics growth. cultures was modulated using Bafilomycin A1 (BafA1) metabolic traits were analyzed live assays. GBM orthotopic xenografts used vivo models of disease. Cell extracts, proximity-ligation...
Human epidermal growth factor receptor 2 (HER2) is overexpressed/amplified in one third of breast cancers (BCs), and associated with the poorer prognosis higher metastatic potential BC.Emerging evidences highlight role microRNAs (miRNAs) regulation several cellular processes, including BC. Methods: Here we identified, by silico approach, a group three miRNAs central biological (high degree centrality) HER2+ BC.We validated their dysregulation BC analysed functional vitro approaches on...
Maternal obesity and gestational diabetes mellitus (GDM) are increasing worldwide, representing risk factors for both mother child short/long-term outcomes. Oxidative stress, lipotoxicity altered autophagy have already been reported in obesity, but few studies focused on obese pregnant women with GDM. Antioxidant macro/chaperone-mediated (CMA)-related gene expressions were evaluated herein GDM placentas. A total of 47 singleton pregnancies delivered by elective cesarean section enrolled: 16...
Grana Padano (GP) is an Italian hard cooked cheese characterized by a long ripening process and high protein Ca contents. After in vitro static simulated gastrointestinal digestion, GP digest contained caseinophosphopeptides that were 6 to 24 amino acids length, including tri-phosphorylated species incorporating the pSer-pSer-pSer-Glu-Glu cluster. Using rat ileum tissue, was used assess absorption ex vivo, which showed significantly better results for comparison CaCO3 aqueous solution. An...
Electrospinning is a versatile method for preparing functional three-dimensional scaffolds. Synthetic and natural polymers have been used to produce micro- nanofibers that mimic extracellular matrices. Here, we describe the use of emulsion electrospinning prepare blended fibers capable hosting aqueous species releasing them in solution. The existence an non-aqueous phase allows water-soluble molecules be introduced without altering structure degradation fibers, means their release properties...
BackgroundCancer cells use specific V-ATPase subunits to activate oncogenic pathways. Therefore, we investigated deregulation in aggressive gliomas and associated signaling.MethodsV-ATPase genes expression pathways were analyzed different series of glioma available from public databases, as well patients' cohort. Activation was at gene protein levels. A genetic model Drosophila melanogaster mice with GBM patients-derived orthotopic xenografts used vivo models disease.FindingsGBM recurrent...
Aim: To study the specificity of cellular MRI based on superparamagnetic iron oxide particles (SPIOs), especially within CNS. Materials & methods: A microglial cell line was engineered for expression a suicide gene, receptor diphtheria toxin (DT), and two reporter genes, green fluorescent protein luciferase, in order to induce, controlled manner, death test it through bioluminescence. SPIO-labeled DT-sensitive control DT-insensitive cells were transplanted into brains mice, which underwent...