Adriamycin and docetaxel are two agents commonly used in treatment of breast cancer, but their efficacy is often limited by the emergence chemoresistance. Recent studies indicate that exosomes act as vehicles for exchange genetic cargo between heterogeneous populations tumor cells, engendering a transmitted drug resistance cancer development progression. However, specific contribution cancer-derived poorly understood. Here we reinforced other's report human cell line MCF-7/S could acquire...

Acquired drug resistance is a major obstacle to chemotherapy of cancers. In this study, we aim investigate the role exosomes in drug-resistance transfer between breast cancer cells and detect probable mechanism. A docetaxel-resistant variant MCF-7 cell line (MCF-7/DOC) was established then compared with drug-sensitive (MCF-7/S). Exosomes were expelled from supernatant using ultracentrifugation. Drug assessed by apoptosis assay MTT examination. Expressions P-glycoprotein (P-gp) analyzed flow...

Breast cancer (BCa) remains chemo-unresponsive by inevitable progression of resistance to first-line treatment with docetaxel (doc). Emerging studies indicate that exosomes act as mediators intercellular communication between heterogeneous populations tumor cells, engendering a transmitted drug for development. Such modulatory effects have been related the constant shuttle biologically active molecules including microRNAs (miRNAs). Here, we aimed investigate relevance exosome-mediated miRNA...

Breast cancer (BCa) is one of the major deadly cancers in women. However, treatment BCa still hindered by acquired-drug resistance. It increasingly reported that exosomes take part development, metastasis, and drug resistance BCa. specific role poorly understood. In this study, we investigate whether transmit through delivering miR-222. We established an adriamycin-resistant variant Michigan Cancer Foundation-7 (MCF-7) breast cell line (MCF-7/Adr) from a drug-sensitive (MCF-7/S). Exosomes...

While adriamycin (adr) offers improvement in survival for breast cancer (BCa) patients, unfortunately, drug resistance is almost inevitable. Mounting evidence suggests that exosomes act as a vehicle genetic cargo and constantly shuttle biologically active molecules including microRNAs (miRNAs) between heterogeneous populations of tumor cells, engendering resistance-promoting niche progression. Our recent study showed from docetaxel-resistance BCa cells could modulate chemosensitivity by...

Exosome-mediated intercellular communication is considered to be an effective mode for malignant cells transform biological behaviors in stromal cells. However, the mechanisms by which exosomes modulate macrophages within tumor microenvironment remain largely unclear. In this study, we found that both adriamycin-resistant breast cancer (BCa) and corresponding (A/exo) were capable of inducing M2 polarization, promoted mobility, proliferation, migration invasion BCa Since deliver microRNAs...

Abstract Exosomal long non-coding RNAs (lncRNAs) are crucial factors that mediate the extracellular communication in tumor microenvironment. DOCK9 antisense RNA2 (DOCK9-AS2) is an exosomal lncRNA which has not been investigated papillary thyroid carcinoma (PTC). Based on result of differentially expressed lncRNAs PTC via bioinformatics databases, we discovered DOCK9-AS2 was upregulated PTC, and presented elevation plasma exosomes patients. Functionally, knockdown reduced proliferation,...

Adriamycin (ADR) is an important chemotherapeutic agent frequently used in treatment of breast cancer. However, resistance to ADR results failure many patients. Recent studies have indicated that microRNAs (miRNAs) may play role such drug-resistance. In the present study, microRNA-452 (miR-452) was found be significantly down-regulated adriamycin-resistant MCF-7 cells (MCF-7/ADR) compared with parental by miRNA microarray and real-time quantitative PCR (RT-qPCR). MiR-452 mimics inhibitors...

Abstract Aims: Baculoviral inhibitor of apoptosis repeat containing 5 (BIRC5) plays vital roles in carcinogenesis by influencing cell division and proliferation inhibiting apoptosis. However, the prognostic significance BIRC5 remains unclear breast cancer. Methods: expression methylation status were evaluated using Oncomine The Cancer Genome Atlas (TCGA) databases. relevance between different clinicopathological features as well survival information was analyzed bc-GenExMiner database...

Abstract Background: Ribonucleotide reductase M2 subunit (RRM2) plays vital roles in many cellular processes such as cell proliferation, invasiveness, migration, angiogenesis, senescence, and tumorigenesis. However, the prognostic significance of RRM2 gene breast cancer remains to be investigated. Methods:RRM2 expression was initially evaluated using Oncomine database. The relevance between level clinical parameters well survival data analyzed Kaplan–Meier Plotter, PrognoScan, Breast Cancer...

Mounting evidence indicate that cuproptosis, a novel form of programmed cell death, contributes to cancer development and progression. However, comprehensive analysis regarding the expressions, functions, regulatory network cuproptosis-related genes is still lacking. In present work, genes, upstream miRNAs lncRNAs, clinical data breast from TCGA database were analyzed by R language including Cox regression analysis, correlation calculation, ROC curve construction, survival evaluation,...

Triple negative breast cancer (TNBC) is a more aggressive subtype of that usually progresses rapidly, develops drug resistance, metastasis, and relapses, remains challenge for clinicians to treat. Programmed cell death (PCD), conserved mechanism suicide controlled by various pathways, contributed carcinogenesis progression. Nevertheless, the prognostic significance PCD-related genes in TNBC largely unclear, accurate models are urgently needed. Gene expression profiles clinical information...

Long non-coding RNAs (lncRNAs), a new type of transcripts, play important roles in various cellular biological processes, involving tumorigenesis. Previous studies showed that lncRNA AFAP1-AS1 was aberrantly expressed numerous cancers. Nevertheless, we know quite little about the expression pattern and function thyroid cancer. In this study, adopted quantitative real-time PCR (qRT-PCR) to detect cancer tissues. We discovered increased MTT assays elucidated down-regulation could suppress...

Abstract Resistance to doxorubicin (DOX) is the most common clinical problem in breast cancer therapy, and underlying molecular mechanism remains be investigated. MicroRNAs (miRNAs) exhibit important regulatory functions various malignant tumors including cancer. The aim of present study was find relationship between miR-222 DOX resistance. We found that highly expressed patients’ serum DOX-resistant cell line MCF-7-R could promote proliferation migration cells. Our results also showed...

MicroRNA-452 (miRNA-452) was overexpressed in docetaxel-resistant human breast cancer MCF-7 cells (MCF-7/DOC). However, its role modulating the sensitivity of to docetaxel (DOC) remains unclear. The aim this study is investigate miRNA-452 DOC.Real-time quantitative PCR (RT-qPCR) were used identify differential expression between MCF-7/DOC and cells. MiRNA-452 mimic transfected into inhibitor these evaluated using RT-qPCR, MTT assay, flow cytometry assay. relationship predictive target gene...

A major cause of failure in chemotherapy is drug resistance cancer cells. Exosomes have been introduced to spread chemoresistance through delivering miRNAs. However, a systematic evaluation the exosomal miRNA expression profiles responsible for still lacking. In present study, signature differentially expressed exosomes derived from adriamycin-resistant (A/exo) and parental breast cells (S/exo) were analyzed by microarray results confirmed PCR. total 309 miRNAs increased 66 decreased...

GTPase activating proteins (RhoGAPs) serve significant roles in multiple aspects of tumor biology. Genes encoding RhoGAPs (ARHGAP), which switch off Rho-like GTPases, are responsible for breast cancer biogenesis. However, the identification suitable and novel biomarkers precision treatment prognosis remains challenging. The present study aimed to evaluate expression ARHGAP family genes investigate survival data using Oncomine, Kaplan-Meier Plotter, bcGenExMiner cBioPortal online databases....

Abstract Background: Tripartite motif 13 (TRIM13) plays a significant role in various biological processes including cell growth, apoptosis, transcriptional regulation, and carcinogenesis. However, the prognostic significance of TRIM13 gene breast cancer treatment remains largely unclear. Methods: We performed bioinformatics analysis clinical parameters survival data as it relates to patients using several online databases Oncomine, bcGenExMiner, PrognoScan, UCSC Xena. Results: found that...