A5034395491- Thyroid Disorders and Treatments
- Thyroid Cancer Diagnosis and Treatment
- Hormonal Regulation and Hypertension
- Adrenal and Paraganglionic Tumors
- MicroRNA in disease regulation
- Parathyroid Disorders and Treatments
- Pituitary Gland Disorders and Treatments
- Cancer-related molecular mechanisms research
- Hypothalamic control of reproductive hormones
- RNA modifications and cancer
- Thyroid and Parathyroid Surgery
- Biochemical Analysis and Sensing Techniques
- Growth Hormone and Insulin-like Growth Factors
- Ion channel regulation and function
- Electrolyte and hormonal disorders
- Otolaryngology and Infectious Diseases
- Genetic and Kidney Cyst Diseases
- Regulation of Appetite and Obesity
- Sympathectomy and Hyperhidrosis Treatments
- Circular RNAs in diseases
- Magnesium in Health and Disease
- Redox biology and oxidative stress
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Protein Kinase Regulation and GTPase Signaling
- Renin-Angiotensin System Studies
Medical University of Warsaw
2004-2024
University Clinical Centre
2023
Université Libre de Bruxelles
2004
Brigham and Women's Hospital
2001
National Academy of Medicine
2001
University of Pittsburgh
1997-1999
Prior work has shown that heterozygosity G/C of single nucleotide polymorphism (SNP rs2910164) within the precursor microRNA-146a predisposes to PTC (odds ratio = 1.62, P 0.000007) although mechanism was unclear. Here, we show GC heterozygotes differ from both GG and CC homozygotes by producing 3 mature microRNAs: 1 leading strand (miR-146a), 2 passenger (miR-146a*G miR-146a*C), each with its distinct set target genes. TaqMan analysis paired tumor/normal samples revealed 1.5- 2.6-fold...
The type 2 5'-deiodinase (D2) appears to play an important role in maintaining the intracerebral T3 content relatively constant during changes thyroidal state. Previous studies have demonstrated that regulation of this enzyme by thyroid hormone and its analogs occurs at a posttranslational level. availability rat D2 complementary DNA now allows assessment whether pretranslational also cerebral cortex. In rats rendered hypothyroid addition methimazole drinking water, messenger RNA (mRNA) is...
A wide use of imaging techniques results in more frequent diagnosis adrenal incidenataloma.To analyse the current state knowledge on incidentaloma adults order to prepare practical management recommendations.Following a discussion, Polish Society Endocrinology expert working group have analysed available data and summarised analysis form recommendations.Unenhanced computed tomography (CT) may be recommended as an initial assessment examination helpful differentiation between adenomas...
Loss of the thyroid hormone receptor is common in tumors. In mouse models, a truncated THRB gene leads to cancer. Previously, we observed up-regulation expression eight microRNAs (miRs) papillary carcinoma (PTC) tumors.Our objective was determine whether might be inhibited by miRs up-regulated PTC.The potential binding miR 3'-untranslated region analyzed silico. Direct inhibition cloned evaluated using luciferase assays. Inhibition endogenous and its target genes (DIO1 APP) examined cell...
Abstract —Thyroid hormone regulation of the cardiac pacemaker gene, hyperpolarization-activated cyclic nucleotide-gated channel gene (HCN2), was studied in rats by Northern analysis. Thyroid administration to hypothyroid resulted a doubling HCN2/β-actin mRNA ratio. A smaller, not statistically significant, increase HCN2 occurred when euthyroid animals were made hyperthyroid. single large dose l -triiodothyronine given caused 4.7-fold myocardial expression level and only 2.3-fold β-actin...
Type 2 iodothyronine deiodinase (D2) catalyzes intracellular 3, 5, 3′ triiodothyronine (T3) production from thyroxine (T4), and its messenger RNA mRNA is highly expressed in human, but not rodent, myocardium. The goal of this study was to identify the effects D2 expression mouse myocardium on cardiac function gene expression. We prepared transgenic (TG) mice which human driven by α-MHC promoter. Despite high myocardial activity, T3 was, at most, minimally increased TG Although, plasma T4,...
The physiological consequences and mechanism(s) for thyroid hormone-induced alterations in serum leptin are not known. To address this, expression rats was evaluated relationship to food intake, fat mass, body temperature with pharmacologically altered status. Total weight, were decreased hypothyroid rats. Fat weight both chronically hyperthyroid (n = 6/group). Serum linearly correlated epididymal retroperitoneal mRNA concentration, but total weight. the When used as a covariant, different...
Phaeochromocytoma is one of the numerous causes secondary hypertension. Furthermore, phaeochromocytoma may first present with type 2 diabetes mellitus. The objective our study was to evaluate effects adrenalectomy on patient recovery regards normotension and well-controlled glycaemia.The retrospective analysis involved 67 patients operated between 2006 mid-2012. pre-operative diagnoses were made in departments internal medicine endocrinology. Based laboratory tests diagnostic imaging, we...
Abstract Thyroid hormone is essential for brain development. T4 has to be converted T3 efficient binding thyroid receptors. Type 2 deiodinase (D2) the key enzyme that allows generation in brain. To elucidate onset and localization of production brain, we studied changes D2 activity, mRNA content, distribution chicken embryos before after function. activity was detectable at all stages from embryonic day (E)7 E15 increased significantly with time. The wild-type transcript those by RT-PCR....
Type 1 and type 2 iodothyronine 5' deiodinases (D1 D2, respectively) catalyze the conversion of thyroxine (T(4)) to triiodothyronine (T(3)). Similar other genes crucial for T(3) generation, D1 D2 expression might be disturbed in papillary thyroid cancer (PTC) possible as a result impairments transcription factors Titf1/Nkx2-1 Pax-8. The aim study was investigate changes PTC compared Although activities were decreased tumor samples control C (tissues from nontumorous part gland), differences...
Type 1 5′-deiodinase is one of two isoenzymes that participate in conversion prohormone thyroxine into triiodothyronine (T3). A decrease type expression was observed renal clear cell carcinoma, thyroid cancer, and lung cancer. The aim this study to evaluate activity mRNA level breast cancer tissue non-cancerous surrounding tissue. Material collected from 36 patients undergoing radical mastectomy or local tumor resection. In all tissues, found be at a very low immeasurable level, detected...
Whereas in the past a negative diagnostic 131I whole body scan (WBS) was interpreted as lack of significant residual or recurrent thyroid cancer, today patient with WBS and measurable serum thyroglobulin (Tg) presents therapeutic dilemma. Previous studies have shown high rate visualization uptake decrease Tg after one more doses 131I. In order to further assess significance this finding, retrospective analysis patients persistent thyroglobulinemia performed for evidence surgically amenable...
Previous studies showed that chronically stressed and myocardially infarcted rats respond with exaggerated cardiovascular responses to acute stress. The present experiments were designed elucidate whether this effect can be abolished by treatment the angiotensin converting enzyme (ACE) inhibitor captopril. Sprague Dawley subjected either sham surgery (Groups 1 2) or myocardial infarction 3 4). of Groups 2 4 also exposed mild chronic stressing. Four weeks after operation, mean arterial blood...
Sialylation of cell components is an important immunomodulating mechanism affecting response to hormones and adhesion molecules. To study alterations in sialic acid metabolism Graves' disease (GD) we measured the following parameters various human thyroid tissues: lipid-bound (LBSA) content, ganglioside profile, total sialyltransferase activity, two major mRNAs for sialyltransferase-1 (ST6Gal I) sialyltransferase-4A (ST3Gal I). Fragments toxic nodules (TN), nontoxic (NN) nontumorous tissue...
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Type 2 iodothyronine deiodinase (D2) catalyzes intracellular 3, 5, 3′ triiodothyronine (T3) production from thyroxine (T4), and its messenger RNA mRNA is highly expressed in human, but not rodent, myocardium. The goal of this study was to identify the effects D2 expression mouse myocardium on cardiac function gene expression. We prepared transgenic (TG) mice which human driven by α-MHC promoter. Despite high myocardial activity, T3 was, at most, minimally increased TG Although, plasma T4,...
Milk-alkali syndrome (MAS), characterized by renal failure, metabolic alkalosis and hypercalcemia, is a severe life-threatening complication of the treatment hypoparathyroidism. The clinical course often sudden not preceded any prodromal symptoms. Occurrence does depend on duration hypoparathyroidism treatment, although it closely related to applied therapy, especially dose calcium carbonate active vitamin D preparations. Drugs influencing glomerular filtration rate (angiotensin receptor...
Hypocalcaemia is a common postoperative complication, both after the resection of parathyroid adenoma associated with primary hyperparathyroidism and total thyroidectomy due to thyroid cancer or nodular goitre. For few years, in patients hypoparathyroidism severe hypocalcaemia, who cannot discontinue intravenous calcium preparations even use high vitamin D doses, attempts have been made add recombinant human parathormone (rhPTH) treatment schedule. In this work, for first time Poland, we...
Transcription factors TTF-1 and Pax-8 control the expression of thyroid-specific genes crucial for thyroid function. It has been postulated that they may play a role in thyrotropin (TSH)-mediated augmentation gene observed some diseases including Grave's hyperthyroidism. Recently, we others described two participating hormone metabolism type I II deiodinase (D1 D2, respectively) are upregulated by TSH, although mechanisms responsible this effect likely to be different. The aim study was...