A5013383103- Chronic Obstructive Pulmonary Disease (COPD) Research
- Pediatric health and respiratory diseases
- Asthma and respiratory diseases
- Neonatal Respiratory Health Research
- Respiratory Support and Mechanisms
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Angiogenesis and VEGF in Cancer
- Respiratory viral infections research
- Apelin-related biomedical research
- Inhalation and Respiratory Drug Delivery
- Heat shock proteins research
- Pulmonary Hypertension Research and Treatments
- Peptidase Inhibition and Analysis
- Spaceflight effects on biology
- Coronary Interventions and Diagnostics
- Autophagy in Disease and Therapy
- Protease and Inhibitor Mechanisms
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Nitric Oxide and Endothelin Effects
- IL-33, ST2, and ILC Pathways
- NF-κB Signaling Pathways
- Cardiovascular Disease and Adiposity
- Immune Response and Inflammation
- Lipid metabolism and disorders
- Cardiac Fibrosis and Remodeling
Istituti Clinici Scientifici Maugeri
2008-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2000-2023
Fondazione Salvatore Maugeri
2000-2016
Azienda Ospedaliero Universitaria Ospedali Riuniti
2004
The expression of nuclear factor (NF)-κB is an indicator cellular activation and inflammatory mediator production. aim the present study was to characterise localisation p65, major subunit NF-κB, in bronchial mucosa patients with chronic obstructive pulmonary disease (COPD), examine relationship between p65 status. Bronchial biopsies were obtained from 14 smokers COPD, 17 normal lung function 12 nonsmokers function. number positive (+) cells quantified by immunohistochemistry three groups...
Summary There are increased numbers of activated T lymphocytes in the bronchial mucosa stable chronic obstructive pulmonary disease (COPD) patients. helper type 17 (Th17) cells release interleukin (IL)-17 as their effector cytokine under control IL-22 and IL-23. Furthermore, Th17 some inflammatory conditions. To investigate expression (IL)-17A, IL-17F, IL-21, IL-23 retinoic orphan receptor RORC2, a marker cells, biopsies from patients with COPD different severity compared age-matched...
Information on the clinical traits associated with bronchial neutrophilia in asthma is scant, preventing its recognition and adequate treatment. We aimed to assess clinical, functional biological features of neutrophilic identify possible predictors neutrophilia.The inflammatory phenotype 70 mild-to-severe patients was studied cross-sectionally based eosinophilic/neutrophilic counts their lamina propria. Patients were classified as or non-neutrophilic. Neutrophilic (neutrophil count cut-off:...
CC-chemokines are chemotactic factors expressed in a wide range of cell types and tissues. The aim this study was to evaluate the involvement airways inflammation patients affected by chronic bronchitis. evaluated, with an immunoassay, concentrations monocyte protein-1 (MCP-1), macrophage inflammatory protein-1alpha (MIP-1alpha) protein-1beta (MIP-1beta), bronchoalveolar lavage fluid (BALF) 12 smokers bronchitis 14 smoking, 15 nonsmoking six exsmoking healthy subjects. MCP-1 significantly...
Activation of the transcription factor signal transducer and activator (STAT)‐4 is critical for differentiation T‐helper 1 cells/type‐1 cytotoxic T‐cells production interferon (IFN)‐γ. Expression STAT4, phospho‐STAT4, IFN‐γ T‐box expressed in (T‐bet) proteins bronchial biopsies bronchoalveolar lavage (BAL)‐derived lymphocytes, obtained from 12 smokers with mild/moderate chronic obstructive pulmonary disease (COPD) (forced expiratory volume one second (FEV ) 59±16% predicted), 14 normal lung...
<h3>Background:</h3> Increased numbers of activated neutrophils have been reported in the bronchial mucosa patients with stable chronic obstructive pulmonary disease (COPD), particularly severe disease. <h3>Objectives:</h3> To investigate expression neutrophilic chemokines and adhesion molecules biopsies from COPD different severity (GOLD stages I–IV) compared age-matched control subjects, smokers normal lung function never smokers. <h3>Methods:</h3> The CCL5, CXCL1, 5, 6, 7 8, CXCR1, CXCR2,...
Toll-like receptors (TLRs) and nucleotide-binding oligomerisation domain (NOD)-like (NLRs) are two major forms of innate immune sensors but their role in the immunopathology stable chronic obstructive pulmonary disease (COPD) is incompletely studied. Our objective here was to investigate TLR NLR signalling pathways bronchial mucosa COPD.Using immunohistochemistry, expression levels TLR2, TLR4, TLR9, NOD1, NOD2, CD14, myeloid differentiation primary response gene 88 (MyD88),...
Background It is increasingly clear that some heat shock proteins (Hsps) play a role in inflammation. Here, we report results showing participation of Hsp60 the pathogenesis chronic obstructive pulmonary diseases (COPD), as indicated by data from both vivo and vitro analyses. Methods Results Bronchial biopsies patients with stable COPD, smoker controls normal lung function, non-smoker were studied. We quantified immunohistochemistry levels Hsp10, Hsp27, Hsp40, Hsp60, Hsp70, Hsp90, HSF-1,...
BackgroundThe expression and localization of transforming growth factor-β (TGF-β) pathway proteins in different compartments the lower airways patients with stable COPD is unclear. We aimed to determine TGF-β protein COPD.MethodsThe components was measured bronchial mucosa peripheral lungs (n = 44), control smokers normal lung function 24), nonsmoking subjects 11) using immunohistochemical analysis.ResultsTGF-β1, TGF-β3, connective tissue factor were significantly decreased bronchiolar...
Background: Heart Failure (HF), a leading cause of morbidity and mortality, represents relevant trigger for the development frailty in elderly. Inflammation has been reported to play an important role HF pathophysiology. Galectin-3 (Gal-3), whose levels increase with aging, exerts activity processes cardiac inflammation fibrosis. The aim present study was investigate potential serve as biomarker patients. Methods: 128 consecutive patients aged 65 older diagnosis systolic underwent assessment...
Background Studies on the inflammatory process in large airways of patients with mild/moderate COPD have shown a prevalent T lymphocyte and macrophage infiltration bronchial mucosa. However, inflammation more severe disease has not been extensively studied. Objective The aim present study was to characterize mucosa subjects severe, compared mild, COPD, examine relationship between airflow limitation numbers Methods We examined biopsies obtained from nine smokers limitation, 14 normal lung...
Macrophage inflammatory protein (MIP)-1 α and MIP-1 β are two CC chemokines that induce lymphocyte migration. preferentially mediates chemotaxis of CD8 rather than CD4 lymphocytes, whereas the reverse is true for . Both these recognize CCR5 as a cellular receptor in T lymphocytes alveolar macrophages. We measured concentrations bronchoalveolar lavage fluid (BALF) 30 subjects affected by different stages pulmonary sarcoidosis 18 healthy normal subjects. also evaluated expression macrophages...
CC chemokines play an important role in the pathogenetic mechanisms of interstitial lung disease, while a downregulation chemokine receptor (CCR)5 fibrotic stages sarcoidosis has been observed. To evaluate involvement and expression CCR5 idiopathic pulmonary fibrosis (IPF) and, more specifically, usual pneumonia, 35 subjects were studied. ligand (CCL)2, CCL3 CCL4 levels measured bronchoalveolar lavage fluid (BALF) 18 nonsmoker control 17 patients affected by IPF. was evaluated alveolar...
The pathophysiology of chronic heart failure (CHF) involves multiple hystologic and molecular alterations. To determine the effects physical training on circulating endothelial progenitor cells (EPCs), angiogenesis (angiogenin, angiopoietin-1 -2, VEGF, Tie-2, SDF-1α) inflammation (IL-6, CRP), we compared data obtained from 11 CHF pts before after 3 months aerobic exercise training, to those 10 non trained (CHF-C group, age 64 + 2 years, NYHA 2). At end study, EPCs count AP-2 serum levels...
HSP60 has been implicated in chronic inflammatory disease pathogenesis, including obstructive pulmonary (COPD), but the mechanisms by which this chaperonin would act are poorly understood. A number of studies suggest a role for extracellular HSP60, since it can be secreted from cells and bind Toll-like receptors; however, effects stimulation have never extensively studied. We investigated (pro- or anti-inflammatory) human bronchial epithelial (16-HBE) alone comparison with oxidative,...
Chronic inflammatory responses in the lung of patients with stable mild-to-severe forms chronic obstructive pulmonary disease (COPD) play a central role definition, comprehension and monitoring state. A better understanding COPD pathogenesis cannot avoid detailed knowledge these changes, altering functional health during progression. We summarize discuss principal functions cells populating large, small airways parenchyma increasing severity comparison healthy control subjects: T B...
A reciprocal link between inflammation, oxidative/nitrosative stress, and endothelial dysfunction has been postulated in chronic heart failure (CHF). The repair mechanisms involved remain to be determined. Our aim was investigate whether there are detectable signs of ongoing angiogenesis serum CHF patients evaluate the correlation with indexes haemodynamic functional impairment. Enzyme-linked immunosorbent assay tests were used quantify angiogenin, angiopoietin-1, angiopoietin-2, vascular...
Notch signaling is involved in the prevention of cell differentiation and fate various organs, including lungs. We aimed to determine transcriptomic protein expression receptors, their ligands, related transcription factors stable COPD. The localization were measured bronchial biopsies individuals with mild/moderate (MCOPD) (n = 18) or severe/very severe (SCOPD) 16) COPD, control smokers (CSs) 13), nonsmokers (CNSs) 11), lung parenchyma those MCOPD CSs 10), CNSs 10) using...
Abstract Background Asthma with neutrophilic/mixed inflammation is a difficult-to-control clinical phenotype. Currently, vascular and matrix airway remodeling in asthma not well known. We aimed to evaluate the differences vascular/smooth muscle/matrix related eosinophilic (EOS) mixed/neutrophilic (MIXED) bronchial phenotypes relation severity exacerbation frequency. Methods In this cross-sectional study, α-SMA + cells (100µM beneath basement membrane [BM]), BM thickness, remodeling-related...
The protease–antiprotease balance is involved in many biological processes, including blood coagulation, tissue remodeling, inflammation and immune responses. aim of this study to determine the between SERPINs some related proteases lungs stable COPD patients. In cross-sectional study, expression localization human (anti-proteases) were measured lung parenchyma mild-moderate (MCOPD, n = 13) patients, control smokers (CS, 14) nonsmokers (CNS, 12) using transcriptome analysis,...
<h3>Background</h3> Vascular remodelling plays a central role in asthma and chronic obstructive pulmonary disease (COPD). Bradykinin (BK) is vasoactive proinflammatory peptide mediating acute responses asthma. We investigated the of angiogenic factors relation to BK receptors COPD. <h3>Methods</h3> Bronchial biopsies from 33 patients with COPD, 24 old (≥50 years) asthma, 18 control smokers, 11 non-smokers, 15 young (≤40yrs) (≤40 10 non-smokers were immunostained for CD31, vascular...
Background: Bone morphogenic proteins (BMPs) and their antagonists are involved in the tissue development homeostasis of various organs. Objective: To determine transcriptomic protein expression BMPs stable COPD. Methods: We measured localization some relevant bronchial biopsies mild/moderate COPD (MCOPD) (n = 18), severe/very severe (SCOPD) 16), control smokers (CS) 13), non-smokers (CNS) 11), lung parenchyma MCOPD 9), CS CNS 9) using immunohistochemistry transcriptome analysis, vitro after...